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The map label for this gene is alkK [H]

Identifier: 78066677

GI number: 78066677

Start: 2255955

End: 2257598

Strand: Reverse

Name: alkK [H]

Synonym: Bcep18194_A5208

Alternate gene names: 78066677

Gene position: 2257598-2255955 (Counterclockwise)

Preceding gene: 78066683

Following gene: 78066676

Centisome position: 61.11

GC content: 67.34

Gene sequence:

>1644_bases
ATGGGTAAGCCGTTGCTGGGCCAGATGATGGGCATGCCGTTGCTGGTGTCCTCGCTGATTTCTCATGCCGCGCGGCACGC
CGGCGATACGGAGATCGTGTCGAAGCGCGTGGAAGGCGATCTGCATCGCTACACGTACCGCGATTGCGAACGCCGCGCGA
AGCAGCTGGCGCAGGCGCTCACGCGGCTCGGCGTCGAATCCGGCGATCGCGTCGGTACGCTTGCGTGGAACGGCTACCGG
CATCTGGAAGCGTATTACGGGATCGGCGGGATGGGCGCCGTGTGCCACACGATCAACCCGCGCCTGTTCCCCGAGCAGAT
CGCGTACATCGTCAATCACGCGGAAGACCGCTACGTCTTCTTCGACATCAATTTCGCACCGCTCGTCGAGGCCATCGCGC
CGCAATGTCCGCATGTGAAGGGCTGGGTCGCGATGACGGACGCGGCGCACCTGCCGTCCGGCACGACGCCGTTCCTCTGC
TACGAAACGCTCGTCGAAGCGGAGGATGGCCACTACGACTGGCCGCGCCTCGACGAGCAGCAGGCGTCGGGGCTCTGCTA
CACGTCGGGCACGACCGGCAACCCGAAGGGCGTGCTGTATTCGCACCGCTCGACCGTGCTGCACGCGTACGGCGCCGCGC
TGCCCGACGCGATGAACCTGTCCGCGATGGACGCCGTGCTGCCCGTCGTGCCGATGTTCCACGTCAACGCGTGGGGGCTG
CCGTACGCGGTACCGCTCACGGGCGGCAAGCTCGTGCTGCCCGGCAAGGATCTCGACGGGAAATCGCTGTACGAGTTAAT
GGAAGCCGAGCGCGTGACGTTCTCTGCGGGCGTGCCGACCGTGTGGCTCGGCCTGCTCAACTACATGCGCGAAGCCGGCG
TGCGCTTCTCGACGCTGAACCGCACGGTGATCGGCGGCTCCGCGTGCCCGCCCGCGATGCTGCGCACCTTCGAGGACGAA
TACGGCGTGCGCGTGATCCACGCATGGGGGATGACCGAGCTGTCGCCGCTCGGCACGCTCGCGAAGCTCAACTGGGCGCA
ATCGCAGCGGCCGCTCGACGCGCAGCGCAAGCTGCTCGAGAAGCAGGGGCGCGTGATCTGCGGCGTCGACATGCGCATTG
TCGGCGACGACGGCCACGAGCTGCCGTGGGACGGCGTCGCGTTCGGCGAACTGCAGGTGCGCGGCCCGTGGGTGATCGAC
CACTACTTTCGCGGCGAGGCGTCGCCGCTGTCGGACGGCTGGTTCCCGACCGGCGACGTCGCGACGATCGACCCCGACGG
CTTCCTGCAGATCACCGACCGCAGCAAGGACGTGATCAAGTCGGGCGGCGAGTGGATCAGCTCGATCGACATCGAGAACG
TCGCGATCGCGCACCCGGGCGTGGCCGAGGCCGCCTGCATCGCCTGTGCACACCCGAAATGGACCGAGCGCCCGCTGCTC
GTCGTGGTGCCGCGCGAAGGGGCGAGCCTGAGCCGCGACGCGCTGCTCGCGTTCTACGAAGGCAAGGTCGCGAAATGGTG
GATTCCCGACGACGTCGTATTCGTCGAATCGCTGCCGCACACCGCGACCGGCAAGCTGCAGAAGCTGAAGCTGCGCGAGA
CGTTCCGCGATTACGTGTTGCCGACGGCGGTCTGCGAGCCGTAA

Upstream 100 bases:

>100_bases
GCCCGCATGGTGGCGCGCGCACCGTGCGCCACGCCCGTCCGGGGCCGCTGACGGGCGCGCCGCAGAGGCACCGATTCGCA
TCCTCACGGAGAACAAGACG

Downstream 100 bases:

>100_bases
CGCCGGGCCCGGGCCCGAAGCGGCTCGTGAAGCGGTCGCCCGATCCGTCCCCGGATCGGGCGACCGGCATCGGGAGCAAA
ATTCAAATTGAACGACCGTG

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MGKPLLGQMMGMPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQALTRLGVESGDRVGTLAWNGYR
HLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVEAIAPQCPHVKGWVAMTDAAHLPSGTTPFLC
YETLVEAEDGHYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
PYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTLNRTVIGGSACPPAMLRTFEDE
YGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRKLLEKQGRVICGVDMRIVGDDGHELPWDGVAFGELQVRGPWVID
HYFRGEASPLSDGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHPGVAEAACIACAHPKWTERPLL
VVVPREGASLSRDALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYVLPTAVCEP

Sequences:

>Translated_547_residues
MGKPLLGQMMGMPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQALTRLGVESGDRVGTLAWNGYR
HLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVEAIAPQCPHVKGWVAMTDAAHLPSGTTPFLC
YETLVEAEDGHYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
PYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTLNRTVIGGSACPPAMLRTFEDE
YGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRKLLEKQGRVICGVDMRIVGDDGHELPWDGVAFGELQVRGPWVID
HYFRGEASPLSDGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHPGVAEAACIACAHPKWTERPLL
VVVPREGASLSRDALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYVLPTAVCEP
>Mature_546_residues
GKPLLGQMMGMPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQALTRLGVESGDRVGTLAWNGYRH
LEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVEAIAPQCPHVKGWVAMTDAAHLPSGTTPFLCY
ETLVEAEDGHYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGLP
YAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTLNRTVIGGSACPPAMLRTFEDEY
GVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRKLLEKQGRVICGVDMRIVGDDGHELPWDGVAFGELQVRGPWVIDH
YFRGEASPLSDGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHPGVAEAACIACAHPKWTERPLLV
VVPREGASLSRDALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYVLPTAVCEP

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=527, Percent_Identity=27.134724857685, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI187761345, Length=526, Percent_Identity=27.5665399239544, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI187761343, Length=526, Percent_Identity=27.5665399239544, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38505220, Length=451, Percent_Identity=24.6119733924612, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI28416953, Length=569, Percent_Identity=24.6045694200351, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.7898832684825, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI58082049, Length=548, Percent_Identity=22.2627737226277, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=22.156862745098, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=548, Percent_Identity=21.7153284671533, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI157311622, Length=548, Percent_Identity=21.7153284671533, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI42544132, Length=517, Percent_Identity=22.2437137330754, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=25.381679389313, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=27.3603082851638, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788107, Length=529, Percent_Identity=26.0869565217391, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786810, Length=359, Percent_Identity=28.6908077994429, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789201, Length=377, Percent_Identity=26.2599469496021, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=520, Percent_Identity=26.3461538461538, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=565, Percent_Identity=24.7787610619469, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=565, Percent_Identity=24.7787610619469, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=565, Percent_Identity=24.7787610619469, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=25.78125, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=544, Percent_Identity=22.6102941176471, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=25.0470809792844, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=542, Percent_Identity=23.9852398523985, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=22.1359223300971, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=23.7451737451737, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=509, Percent_Identity=23.1827111984283, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=380, Percent_Identity=22.8947368421053, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24581924, Length=568, Percent_Identity=26.7605633802817, Blast_Score=163, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18859661, Length=513, Percent_Identity=25.5360623781676, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI19922652, Length=502, Percent_Identity=24.7011952191235, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17933690, Length=502, Percent_Identity=23.5059760956175, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=22.5806451612903, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356441, Length=509, Percent_Identity=22.5933202357564, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921316, Length=478, Percent_Identity=25.1046025104602, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648260, Length=516, Percent_Identity=25, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21358303, Length=476, Percent_Identity=23.109243697479, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=480, Percent_Identity=22.2916666666667, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=21.6962524654832, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648255, Length=501, Percent_Identity=21.3572854291417, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=20.6, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI62472339, Length=403, Percent_Identity=22.0843672456576, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24667959, Length=405, Percent_Identity=22.7160493827161, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667955, Length=405, Percent_Identity=22.7160493827161, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356947, Length=503, Percent_Identity=22.0675944333996, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161076582, Length=509, Percent_Identity=21.0216110019646, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 60145; Mature: 60014

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00139 THIOL_PROTEASE_CYS ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPLLGQMMGMPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQAL
CCCCHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TRLGVESGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVF
HHCCCCCCCCEEEEEECCEEHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCEEE
FDINFAPLVEAIAPQCPHVKGWVAMTDAAHLPSGTTPFLCYETLVEAEDGHYDWPRLDEQ
EECCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHH
QASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
HHCCEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCC
PYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTLN
CEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCEEEECC
RTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRKLLE
EEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHH
KQGRVICGVDMRIVGDDGHELPWDGVAFGELQVRGPWVIDHYFRGEASPLSDGWFPTGDV
HCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEEEEHHHCCCCCCCCCCCCCCCCE
ATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHPGVAEAACIACAHPKWTERPLL
EEECCCCCEEEECCCHHHHHCCCCEEHHCCCCCEEECCCCCHHHHHEEECCCCCCCCCEE
VVVPREGASLSRDALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYVL
EEECCCCCCCCHHHEEEEECCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCC
PTAVCEP
CHHCCCC
>Mature Secondary Structure 
GKPLLGQMMGMPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQAL
CCCHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TRLGVESGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVF
HHCCCCCCCCEEEEEECCEEHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCEEE
FDINFAPLVEAIAPQCPHVKGWVAMTDAAHLPSGTTPFLCYETLVEAEDGHYDWPRLDEQ
EECCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHH
QASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
HHCCEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEECCCCC
PYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTLN
CEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCEEEECC
RTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRKLLE
EEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHCCCCHHHHHHHHH
KQGRVICGVDMRIVGDDGHELPWDGVAFGELQVRGPWVIDHYFRGEASPLSDGWFPTGDV
HCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEEEEHHHCCCCCCCCCCCCCCCCE
ATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHPGVAEAACIACAHPKWTERPLL
EEECCCCCEEEECCCHHHHHCCCCEEHHCCCCCEEECCCCCHHHHHEEECCCCCCCCCEE
VVVPREGASLSRDALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYVL
EEECCCCCCCCHHHEEEEECCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCC
PTAVCEP
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]