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The map label for this gene is aes [H]

Identifier: 78066667

GI number: 78066667

Start: 2242827

End: 2243786

Strand: Reverse

Name: aes [H]

Synonym: Bcep18194_A5198

Alternate gene names: 78066667

Gene position: 2243786-2242827 (Counterclockwise)

Preceding gene: 78066668

Following gene: 78066666

Centisome position: 60.74

GC content: 67.6

Gene sequence:

>960_bases
ATGCCGCTGAATCCGAAGATCGCCCAGGTGCTCGACATGATCGAGCGTGCGAAACGTCCGTCCTATCACCACCAGACGCC
GCAGCAGGCGCGCGCCGCGTACGAGAAGAGCGCGCCGATCCTCGACGTCGCATCGGCGCCGATGCATTCGGTCGAGGAAT
GCGTCGTGCCGACGCGCGACGGCCGCACGATCGGTGCACGGCTGTACCTGCCGGTTGAGCCGAGCCTCGCGGAGCCGCTG
CCCGCGCTCGTCTACTACCATGGCGGCGGCTTCACGGTCGGCAGCGTCGATACACATGATGCGCTGTGCCGGATGTTCGC
GCGCGATGCGCAATGCGCGGTGCTCTCGGTCGGTTACCGGCTCGCGCCGGAACATCGATTCCCGACCGCGGTGAACGACG
CCGACGATGCGTTGCAATGGCTGCATCGCGAGGCCGCCACGTTCGGCATCGACGCCGCACGGCTGGCCGTCGGCGGCGAC
AGCGCGGGCGGCACGCTCGCGACCGTCTGCGCGGTGCTCGCACGCGACGCAGGCATTCGCCTTGCGCTGCAGATGTTGAT
CTATCCGGGCGTGACGGGCTACCAGGACACCGAATCGCACGCACGGCTCGCGAACGGCTACTTGCTGACGCAGGACACGA
TCCAGTGGTTCTTCACGCAATACGTGCGCGATCGGGCAGACCGCGACGACTGGCGTTTCGCGCCGCTCGACGGCACGCGC
GATGCACCGTCGTTCGCGGGTGTCGCGCCGGCGTGGATCGCGACGGCCGAATACGATCCGCTGAGCGACGAAGGCGCCGC
GTATGCGGAGAAGTTGCGTGCGGCGGGCAACGTGGTCACGCTGGTCTGCTATCCGGGGATGATCCACGAATTCTTCAAGA
TGGGCGGCTACGTGCCCGAGGTACGGGCCGCGCATGCCGATGCGGTCGCGGCGCTGAAGGCCGCATTCGACGACGTTTGA

Upstream 100 bases:

>100_bases
GGGATTTTCGATACGCCCGGCGCATAACGCTCACCTGACGCGCGCATGGCATCATGCACGCTTGTCCGTTCACGGCGCCC
CGCAGGCGCCGTCCTAACCG

Downstream 100 bases:

>100_bases
CGGCGGTTGTTGAGCGGCGGACGCGCCGCATGGGAGCTGCGATGACGGACGGCGTGGTGGAAACGGGCGACTGGGTGGTA
CTGGGCGGCGACGCCGCGCG

Product: esterase/lipase/thioesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPLNPKIAQVLDMIERAKRPSYHHQTPQQARAAYEKSAPILDVASAPMHSVEECVVPTRDGRTIGARLYLPVEPSLAEPL
PALVYYHGGGFTVGSVDTHDALCRMFARDAQCAVLSVGYRLAPEHRFPTAVNDADDALQWLHREAATFGIDAARLAVGGD
SAGGTLATVCAVLARDAGIRLALQMLIYPGVTGYQDTESHARLANGYLLTQDTIQWFFTQYVRDRADRDDWRFAPLDGTR
DAPSFAGVAPAWIATAEYDPLSDEGAAYAEKLRAAGNVVTLVCYPGMIHEFFKMGGYVPEVRAAHADAVAALKAAFDDV

Sequences:

>Translated_319_residues
MPLNPKIAQVLDMIERAKRPSYHHQTPQQARAAYEKSAPILDVASAPMHSVEECVVPTRDGRTIGARLYLPVEPSLAEPL
PALVYYHGGGFTVGSVDTHDALCRMFARDAQCAVLSVGYRLAPEHRFPTAVNDADDALQWLHREAATFGIDAARLAVGGD
SAGGTLATVCAVLARDAGIRLALQMLIYPGVTGYQDTESHARLANGYLLTQDTIQWFFTQYVRDRADRDDWRFAPLDGTR
DAPSFAGVAPAWIATAEYDPLSDEGAAYAEKLRAAGNVVTLVCYPGMIHEFFKMGGYVPEVRAAHADAVAALKAAFDDV
>Mature_318_residues
PLNPKIAQVLDMIERAKRPSYHHQTPQQARAAYEKSAPILDVASAPMHSVEECVVPTRDGRTIGARLYLPVEPSLAEPLP
ALVYYHGGGFTVGSVDTHDALCRMFARDAQCAVLSVGYRLAPEHRFPTAVNDADDALQWLHREAATFGIDAARLAVGGDS
AGGTLATVCAVLARDAGIRLALQMLIYPGVTGYQDTESHARLANGYLLTQDTIQWFFTQYVRDRADRDDWRFAPLDGTRD
APSFAGVAPAWIATAEYDPLSDEGAAYAEKLRAAGNVVTLVCYPGMIHEFFKMGGYVPEVRAAHADAVAALKAAFDDV

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=297, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI157041239, Length=161, Percent_Identity=36.6459627329193, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI206597554, Length=172, Percent_Identity=36.6279069767442, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI61966717, Length=205, Percent_Identity=30.7317073170732, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI68051721, Length=272, Percent_Identity=26.8382352941176, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226423947, Length=280, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI157041237, Length=128, Percent_Identity=34.375, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI21328446, Length=121, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1786682, Length=255, Percent_Identity=34.5098039215686, Blast_Score=134, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71996133, Length=166, Percent_Identity=35.5421686746988, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=269, Percent_Identity=30.8550185873606, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=149, Percent_Identity=34.2281879194631, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=122, Percent_Identity=39.344262295082, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656084, Length=122, Percent_Identity=35.2459016393443, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24656076, Length=122, Percent_Identity=35.2459016393443, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20130169, Length=122, Percent_Identity=35.2459016393443, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34591; Mature: 34460

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNPKIAQVLDMIERAKRPSYHHQTPQQARAAYEKSAPILDVASAPMHSVEECVVPTRD
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCCC
GRTIGARLYLPVEPSLAEPLPALVYYHGGGFTVGSVDTHDALCRMFARDAQCAVLSVGYR
CCEEEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEECCCE
LAPEHRFPTAVNDADDALQWLHREAATFGIDAARLAVGGDSAGGTLATVCAVLARDAGIR
ECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCHHHHHHHHHHHCCCHH
LALQMLIYPGVTGYQDTESHARLANGYLLTQDTIQWFFTQYVRDRADRDDWRFAPLDGTR
HHHHHHHHCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DAPSFAGVAPAWIATAEYDPLSDEGAAYAEKLRAAGNVVTLVCYPGMIHEFFKMGGYVPE
CCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCH
VRAAHADAVAALKAAFDDV
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLNPKIAQVLDMIERAKRPSYHHQTPQQARAAYEKSAPILDVASAPMHSVEECVVPTRD
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCCC
GRTIGARLYLPVEPSLAEPLPALVYYHGGGFTVGSVDTHDALCRMFARDAQCAVLSVGYR
CCEEEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEECCCE
LAPEHRFPTAVNDADDALQWLHREAATFGIDAARLAVGGDSAGGTLATVCAVLARDAGIR
ECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCHHHHHHHHHHHCCCHH
LALQMLIYPGVTGYQDTESHARLANGYLLTQDTIQWFFTQYVRDRADRDDWRFAPLDGTR
HHHHHHHHCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DAPSFAGVAPAWIATAEYDPLSDEGAAYAEKLRAAGNVVTLVCYPGMIHEFFKMGGYVPE
CCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCH
VRAAHADAVAALKAAFDDV
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA