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The map label for this gene is holB [H]

Identifier: 78066661

GI number: 78066661

Start: 2237603

End: 2238631

Strand: Reverse

Name: holB [H]

Synonym: Bcep18194_A5192

Alternate gene names: 78066661

Gene position: 2238631-2237603 (Counterclockwise)

Preceding gene: 78066662

Following gene: 78066660

Centisome position: 60.6

GC content: 70.94

Gene sequence:

>1029_bases
ATGATCTATCCGTGGCAGACCGACGACTGGAACCGCCTGCAGCAACTGCGTGCGCAATGGCCGCACGCGCTGTTGCTGCA
CGGTCAGGCCGGGATCGGCAAGCTGCAGTTCGCACAGCATCTCGCGCAGGGCTTCCTGTGCGAATCGCCGCAGCCGAACG
GCGAGCCGTGCGGCACCTGCGCGGCCTGCACCTGGTTCTCGCAGGGCAACCATCCGGACTACCGGATCGTGCTGCCGGAA
GCGCTGGCGGGCGAGGCGCCGGGCGCCGCGGACGACGCGAAGGCGGCCGATGCGGACGAAGGCGGCAAGAAGACGCGGGC
GCCGAGCAAGGAGATCAAGATCGAGCAGGTGCGCGCACTACTCGACTTCTGCGGGGTCGGCTCGCATCGCGGCGGCGCGC
GCGTCGTCGTGCTGTATCCGGCCGAGGCACTCAACGTCGCCGCGTCGAACGCGCTGCTGAAAACGCTGGAGGAGCCGCCG
TCCGGCGTCGTGTTCCTGCTCGTGTCGGCGCGCATCGACCGCCTGCTGCCGACCATCATCAGCCGCTGCCGGCAGTGGCC
GATGACCGTGCCGGCGCCCGACGCGGCCGCGGCCTGGCTCGCCGCACAGGGCGTCGATCATGCGCCTGCACTGCTCGCCG
AGGTCGGTGGTGCGCCGCTCGCTGCGCTGGCGCTCGCGAGCGACGAGAACCGCCCGCTGCGCGACTACACGCTCGGGCAG
CTCGCGGCCGGTGCGGCCTGCGACCCGTTCGCGTGCGGCGAGACGCTGCAGAAGCTGCCGGTGCCGCTGGTGCTCGGCTG
GCTGCAGCGCTGGCTGTACGACCTGCTTGCCCAGCGGATGGCGGGCGCGCCGCGCTACTTCCCGGCGCACGCGGCGGCGC
TCGCGCGCTGTGCGGAAGCGGTCGACGCGAACGCGTTCGCCCGCTTCATGAAGGCCGTGACGCGGCAGCGGACGGTCGAG
AACCATCCGCTCAACGCGCGGCTCGTATTCGAGGAATTGTTTCTCGGATACCGGGAAATGTTCGCGTGA

Upstream 100 bases:

>100_bases
CACCGCTTCGCGATCGTCGATGCGACGCAGACGATTCCCGAGATTCGCCATCAGCTCGAACGCGTGCTCGCCGCGCTGTA
ATTCGCAAGGAACGCACCAC

Downstream 100 bases:

>100_bases
TCCGGCATTGTTTCGTGTGGGTTGCGAAACGATGACCGGCGCACGCCGCGACGCGACCGTGGCCGTCCGGCCCGCGAACG
CCGCCCGTTGCCGCATGCCG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLAQGFLCESPQPNGEPCGTCAACTWFSQGNHPDYRIVLPE
ALAGEAPGAADDAKAADADEGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGARVVVLYPAEALNVAASNALLKTLEEPP
SGVVFLLVSARIDRLLPTIISRCRQWPMTVPAPDAAAAWLAAQGVDHAPALLAEVGGAPLAALALASDENRPLRDYTLGQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAEAVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA

Sequences:

>Translated_342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLAQGFLCESPQPNGEPCGTCAACTWFSQGNHPDYRIVLPE
ALAGEAPGAADDAKAADADEGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGARVVVLYPAEALNVAASNALLKTLEEPP
SGVVFLLVSARIDRLLPTIISRCRQWPMTVPAPDAAAAWLAAQGVDHAPALLAEVGGAPLAALALASDENRPLRDYTLGQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAEAVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA
>Mature_342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLAQGFLCESPQPNGEPCGTCAACTWFSQGNHPDYRIVLPE
ALAGEAPGAADDAKAADADEGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGARVVVLYPAEALNVAASNALLKTLEEPP
SGVVFLLVSARIDRLLPTIISRCRQWPMTVPAPDAAAAWLAAQGVDHAPALLAEVGGAPLAALALASDENRPLRDYTLGQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAEAVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=289, Percent_Identity=31.8339100346021, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36927; Mature: 36927

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLAQGFLCESPQPNGEPCGTC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFSQGNHPDYRIVLPEALAGEAPGAADDAKAADADEGGKKTRAPSKEIKIEQVRAL
HHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDFCGVGSHRGGARVVVLYPAEALNVAASNALLKTLEEPPSGVVFLLVSARIDRLLPTII
HHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMTVPAPDAAAAWLAAQGVDHAPALLAEVGGAPLAALALASDENRPLRDYTLGQ
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHEECCCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAEA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDANAFARFMKAVTRQRTVENHPLNARLVFEELFLGYREMFA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLAQGFLCESPQPNGEPCGTC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFSQGNHPDYRIVLPEALAGEAPGAADDAKAADADEGGKKTRAPSKEIKIEQVRAL
HHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDFCGVGSHRGGARVVVLYPAEALNVAASNALLKTLEEPPSGVVFLLVSARIDRLLPTII
HHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMTVPAPDAAAAWLAAQGVDHAPALLAEVGGAPLAALALASDENRPLRDYTLGQ
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHEECCCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAEA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDANAFARFMKAVTRQRTVENHPLNARLVFEELFLGYREMFA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]