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The map label for this gene is rlmN [H]

Identifier: 78066584

GI number: 78066584

Start: 2158503

End: 2159642

Strand: Reverse

Name: rlmN [H]

Synonym: Bcep18194_A5115

Alternate gene names: 78066584

Gene position: 2159642-2158503 (Counterclockwise)

Preceding gene: 78066585

Following gene: 78066583

Centisome position: 58.46

GC content: 63.86

Gene sequence:

>1140_bases
ATGACGAGCGAAACTACCGTCAATCTTCTCGACTTCGATGCCGAGGGTCTCGTCGCGTACTGCGGCAGCCTCGGCGAGAA
GCCGTTCCGCGCCAAGCAGTTGCAGCGCTGGATCCATCAGTACAACGCTGGCGACTTCGACGGCATGACCGATCTCGCGA
AGTCCTTGAGAGAGAAACTCAAGGGCCGCGCGTCGATCGGCATGCCCGAGATCGCCAGCGATCACGTTTCCACCGACGGC
ACGCGCAAGTGGCTGATCGACGTCGGAAACGGCAATGCGGTCGAAACCGTGTTCATCCCGGAGGAGACGCGCGGCACGCT
GTGCGTGTCGTCGCAGGCCGGATGTGCGGTCAACTGCCGCTTCTGTTCGACGGGCAAGCAGGGCTTTTCCCGCAATCTGT
CGACAGCCGAAATCATCGGCCAGCTACGGATGGCCGAATTTGCATTGCGAGCGTCACTGGGCCGCGCGCCCGGCCCGAAC
GGCAAGGCCGAACGGGTCGTCACCAACGTGGTGATGATGGGCATGGGCGAACCGCTGCTGAATTACAGCGCGGTCGTGCC
GGCGATGCGGCTGATGCTCGACGACAACGCGTACGGCCTGTCGCGCCGCCGCGTGACGCTGTCGACGTCCGGCGTGGTGC
CGATGATGGACCGCCTCGGTGCCGAGCTGCCGGTCGCGCTGGCCGTTTCGCTGCACGCGCCGAACGATCCGCTGCGCGAC
GAGCTGGTGCCGCTCAACAAGAAGTATCCGCTGCGCGAGCTGATGGCGGCTTGCCAGCGTTATCTGAAAGTCGCGCCGCG
CGACTTCATTACTTTCGAATACTGCATGCTGGACGGCGTCAACGACACCGAAGCGCATGCGCGCGAACTGCTGGCCGTCA
CGCGCGACGTGCCGTGCAAGTTCAATCTGATCCCGTTCAATCCGTTTCCGGAATCGGGCCTCATCCGCTCGAAGCCGGAG
CAGATCAAGCGTTTTGCACAGGTCCTCATCGACGCGGGTGTCGTCACGACCGTGCGCAAGACGCGCGGCGACGACATCGA
TGCCGCGTGCGGCCAGTTGGCCGGCGCAGTGAAGGACCGCACGCGCCTCGCGGAACGTACGGGCGCAGCAGGAAAAATCA
TCGAAGTTCGGGCGATTTGA

Upstream 100 bases:

>100_bases
GAAGTCGCGTTCTTCTTCCCGGAAATGAACGTTTACTCGCGCTAAGCCCTGGCTGGCAGCAAGTAGGCAGGATTCAGCGC
ACGCGGCAAGGCAGCACATC

Downstream 100 bases:

>100_bases
CGGGGACGCCTAGCCGGGGATGCTGGGGACCTTTAAGCGGTACAACACGCGAATAGCGATCGGTCGCGGCGCCTCGGCGT
CGCGCATGGTATGGACAGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTSETTVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIGMPEIASDHVSTDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDPLRD
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAI

Sequences:

>Translated_379_residues
MTSETTVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIGMPEIASDHVSTDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDPLRD
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAI
>Mature_378_residues
TSETTVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKLKGRASIGMPEIASDHVSTDGT
RKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNG
KAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDPLRDE
LVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPEQ
IKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAAGKIIEVRAI

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=380, Percent_Identity=53.9473684210526, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004383 [H]

Pfam domain/function: NA

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41364; Mature: 41233

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSETTVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKL
CCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASIGMPEIASDHVSTDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDPLRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCHH
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAAGKIIEVRAI
HHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
TSETTVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGDFDGMTDLAKSLREKL
CCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASIGMPEIASDHVSTDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDPLRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCHH
ELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQVLIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAAGKIIEVRAI
HHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA