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The map label for this gene is purA1

Identifier: 78066571

GI number: 78066571

Start: 2144181

End: 2145527

Strand: Reverse

Name: purA1

Synonym: Bcep18194_A5102

Alternate gene names: 78066571

Gene position: 2145527-2144181 (Counterclockwise)

Preceding gene: 78066572

Following gene: 78066570

Centisome position: 58.08

GC content: 64.88

Gene sequence:

>1347_bases
ATGTCTGCCAGCGCAGTGAACGTGACTCCCGGGCGCAATGTCGTCGTCGTGGGAACGCAATGGGGTGATGAAGGCAAGGG
CAAGATCGTCGACTGGCTGACGGACCACGCTCAGGGCGTCGTGCGTTTCCAGGGCGGTCACAACGCCGGCCACACCCTCA
TCATCGGCGGCAAGAAAACGATCTTGCGCCTCATTCCGTCGGGCATCATGCGTGAAGGCGTCGCCTGCTACATCGGCAAC
GGCGTCGTCCTGTCCCCCGAAGCACTGTTCAAGGAAATCGGCGAGCTGGAAGAAGCCGGCGTGAACGTGCGCGACCGCCT
GTTCATTTCCGAAGCGACGACGCTGATCCTGCCGTATCACATCGCGATCGACCAGGCGCGCGAAGCGCGCAAGGGCGCGG
GCAAGATCGGCACGACGGGCCGCGGCATCGGCCCCGCGTACGAAGACAAGGTCGGCCGCCGCGCACTGCGCGTGCAGGAC
CTGTTCGACGCGAAGACCTTCGCCGACCGCCTGCGCGAAAACCTCGATTTCCACAACTTCGTGCTGACCCAGTACCTGGG
TGGCGCGGCCGTCGATTTCCAGGCCACGCTCGACACGATGCTCGGCTATGCCGACCGCCTGAAGCCGATGGTGGCCGACG
TGTCGCGCCGCCTGTACGACGCGAACAATGCGGGCCAGAACCTGCTGTTCGAAGGCGCACAAGGCACGCTGCTCGACATC
GATCACGGCACCTATCCGTTCGTCACGTCGAGCAACTGCGTCGCAGGTGCGGCGTCGGCTGGCGCGGGCGTGGGTCCGCA
GAAGCTCAACTACATCCTCGGCATCACGAAGGCATATTGCACGCGCGTCGGCTCGGGCCCGTTCCCGAGCGAACTGTACG
ATGCGGACAATCCGCAGCGTCAGGACCAGGTCGGCGTCACGCTCGCGAACGTCGGCAAGGAATTCGGTTCGGTCACCGGC
CGTCCGCGTCGCACGGGCTGGCTCGACGCAGCCGCGCTGCGCCGCTCGATCCAGATCAACGGCGTGTCGGGCCTGTGCAT
GACGAAGCTCGACGTGCTCGACGGCCTCGACGAAGTCAAGCTGTGCGTCGGCTACAAGATCGACGGCAAGGATGCGGACA
TCCTGCCGCGCGGTGCCGCTGATGTGGCCCGTTGCGAACCTGTATACGAAACGTTTGCCGGCTGGAAGGAAAGCACGGTC
GGCATCAAGACGTGGGAAGCACTGCCGGCGAACGCGCAGGCTTACCTGACCCGCGTCCAGGAAGTGGCTGGCGTGCCGGT
CGACATGGTGTCGACGGGTCCGGACCGCGACGAAACGATTCTGCTCCGCCATCCGTTCAAGGTGTAA

Upstream 100 bases:

>100_bases
GGACGTGCTTCGGCATGCATATCGTTGAAGCGAAACGGAAAAATTCGGAATCGCCCGCGAACATAGGTAGAATACGTTTT
TAACCAGCTAACGAATCAAC

Downstream 100 bases:

>100_bases
TTTCCAGATGACGCAGCAAATCACGATGACGGCGGCAGACTTGATGACCGATCCGCGCAACGACGACAAGAACCTGTGGG
TAGGCTGGGACGAGTATCAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 1; AdSS 1; IMP--aspartate ligase 1

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGN
GVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQD
LFDAKTFADRLRENLDFHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQVGVTLANVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKIDGKDADILPRGAADVARCEPVYETFAGWKESTV
GIKTWEALPANAQAYLTRVQEVAGVPVDMVSTGPDRDETILLRHPFKV

Sequences:

>Translated_448_residues
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGN
GVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQD
LFDAKTFADRLRENLDFHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQVGVTLANVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKIDGKDADILPRGAADVARCEPVYETFAGWKESTV
GIKTWEALPANAQAYLTRVQEVAGVPVDMVSTGPDRDETILLRHPFKV
>Mature_447_residues
SASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGNG
VVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDL
FDAKTFADRLRENLDFHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDID
HGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQVGVTLANVGKEFGSVTGR
PRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKIDGKDADILPRGAADVARCEPVYETFAGWKESTVG
IKTWEALPANAQAYLTRVQEVAGVPVDMVSTGPDRDETILLRHPFKV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=440, Percent_Identity=41.8181818181818, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI34577063, Length=443, Percent_Identity=39.0519187358916, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=40.4938271604938, Blast_Score=289, Evalue=3e-78,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=59.954233409611, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=39.9082568807339, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=39.9082568807339, Blast_Score=308, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=40.5034324942792, Blast_Score=319, Evalue=6e-88,
Organism=Drosophila melanogaster, GI21356033, Length=456, Percent_Identity=39.0350877192982, Blast_Score=314, Evalue=7e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA1_BURS3 (Q39FS0)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_369340.1
- ProteinModelPortal:   Q39FS0
- SMR:   Q39FS0
- STRING:   Q39FS0
- GeneID:   3750310
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A5102
- NMPDR:   fig|269483.3.peg.6177
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- BioCyc:   BSP36773:BCEP18194_A5102-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48133; Mature: 48002

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 23-23 ACT_SITE 51-51 BINDING 139-139 BINDING 153-153 BINDING 234-234 BINDING 249-249 BINDING 321-321 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMREGVACYIGNGVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYH
HHHHHHHHHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEH
IAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDLFDAKTFADRLRENLDFHNF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
QDQVGVTLANVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKIDGKDADILPRGAADVARCEPVYETFAGWKESTVGIKTWEALPANAQAYLTRVQ
EEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
EVAGVPVDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMREGVACYIGNGVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYH
HHHHHHHHHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEH
IAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDLFDAKTFADRLRENLDFHNF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
QDQVGVTLANVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKIDGKDADILPRGAADVARCEPVYETFAGWKESTVGIKTWEALPANAQAYLTRVQ
EEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
EVAGVPVDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA