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The map label for this gene is phbC [H]

Identifier: 78066561

GI number: 78066561

Start: 2130176

End: 2132041

Strand: Reverse

Name: phbC [H]

Synonym: Bcep18194_A5092

Alternate gene names: 78066561

Gene position: 2132041-2130176 (Counterclockwise)

Preceding gene: 78066562

Following gene: 78066560

Centisome position: 57.71

GC content: 65.38

Gene sequence:

>1866_bases
ATGACAGCATCGAAAAAATCGTCGACGTCCGCTCAGGCGGGCACTTCCACGGGCAGCATTGGTTTCGATCCGGCCGCCCA
GCCGATGCAGCAGATGTTCGAGTCGTGGTTGAACGCGTGGCGCGGTTTTGCAGATCCGGCACGCGCCGCGACTGCGTCGG
CCACCGTGAATCCGTTCGCAACATTCCAGTTTCCGACATCGTTTCCGTTCCAGATGCCGTCGATGCCCGATTTCGGCGCG
ATGGCGTCGCCGTTCGCCGGGTTGAAGCTGCCGGCCGCCGCGATTCCGCCCGAGCGGCTGCAGGCGCTGCAGGCCGACTA
CGCGCGCGATTGCATGGCGTTGATGCAGCAGGCCGCCGCGGCGAAGCTCGAGTCGCCCGAACTGAAGGACCGCCGCTTCA
GCGGCGATGCGTGGAAGGCGTCGCCGGCACATGCGTTCGCGGCAGCCTGGTATCTGCTCAACGCGCGCTATCTGCAGGAA
CTGGCCGACGCGCTGCAGACCGATCCGAAGACGCGCGAGCGCATTCGTTTCACGGTCCAGCAGTGGACGGCCGCGGCCGC
GCCGAGCAACTTCCTCGCACTCAATCCCGACGCACAGAAATCGATCCTCGAGACGCAGGGCGAAAGCCTGCGGCAGGGGA
TGATGAACCTGCTCGGCGACATGCAGCGCGGCAAGATTTCGCAGACCGACGAATCGCAGTTCGTGGTCGGCAAGAACCTC
GGGTGTACTGAAGGTGCGGTCGTCTACGAGAACGACCTGATCCAGCTGATCCAGTACACGCCGAAGACGGACAAGGTGTT
CGAGCGGCCGCTGCTGATCGTGCCGCCTTGCATCAACAAGTTCTACATCCTCGACCTGCAGCCGGAGAATTCGCTCGTCG
CGCATGCGCTGTCGAACGGCCATCAGGTGTTCCTCGTGTCGTGGCGCAACGCGGACGCATCGGTCGCGCACAAGACGTGG
GACGACTACATGAACGAAGGGCTGCTCGCGGCGATCGACGCCGTGCAGCAGGTCAGCGGCCGCGAACAGATCAACACGCT
CGGCTTCTGCGTCGGCGGCACGATGCTCGCGACCGCGCTCGCGGTGCTGGCCGCGCGCGGCGAACATCCGGCTGCGTCGA
TGACGCTGCTCACCGCGATGCTCGACTTCACCGACACCGGCATTCTCGACGTGTTCGTCGACGAGGCGCACGTGCAGATG
CGCGAGCAGACCATCGGCGGCAAGAACGGCACGCAGCCGGGGCTGATGCGCGGCGTCGAATTCGCGAACACGTTCTCGTT
CCTGCGGCCGAACGATCTCGTGTGGAACTACGTCGTCGACAACTACCTGAAGGGCCGCACGCCGGCGCCGTTCGACCTGC
TGTACTGGAACAGCGATTCGACGAGCCTGCCGGGCCCGATGTATGCGTGGTACCTGCGCAATACCTATCTCGAGAACAAG
CTGCGCGAACCTGGCGCGCTGACCGTGTGCGGCGAATCGGTCGACCTGACGCTGATCGACGTGCCGACCTTCATCTACGG
CTCGCGCGAGGATCACATCGTGCCGTGGCAGACGGCCTACGCGTCCACGTCGATCCTGTCGGGCCCGCTGAAGTTCGTGC
TCGGCGCGTCGGGACACATCGCGGGCGTGATCAATCCGCCGGCGAAGAAGAAGCGCAGCTACTGGGTCAACGACAGCGAT
CTGCCCGAATCGGCGGACGACTGGTTCGCCGGTGCCACCGAGCAACCGGGCAGCTGGTGGACGACCTGGGCCGAATGGCT
CGACCAGTACGGCGGCCGCAAGGTGGAGCCGCCCGCCCAGCCCGGTACCGCGCAGTTCCCGGTGATCGAGCCGGCGCCGG
GCCGCTACGTGTTGCAGCGCGATTGA

Upstream 100 bases:

>100_bases
AGACGCCTCTCCAGAGCATGACGGCCCGGCTCGCAGCCGGCATGCCGCGCGCGAGCAGGATTTCACGATAGACGCTCACG
AATCACCGGTAAGGCAGGTA

Downstream 100 bases:

>100_bases
CGAAAACCGGACAGCGGCGCAGGCTGTCCGGTGCACCGCAGTTTTTTTAACAGGGCCGGTAGCGATGCGCCGTGTCGTGC
GGGCCTGGAGGAAAGAGAAA

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTASKKSSTSAQAGTSTGSIGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQMPSMPDFGA
MASPFAGLKLPAAAIPPERLQALQADYARDCMALMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQE
LADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDMQRGKISQTDESQFVVGKNL
GCTEGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTW
DDYMNEGLLAAIDAVQQVSGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQM
REQTIGGKNGTQPGLMRGVEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRNTYLENK
LREPGALTVCGESVDLTLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNDSD
LPESADDWFAGATEQPGSWWTTWAEWLDQYGGRKVEPPAQPGTAQFPVIEPAPGRYVLQRD

Sequences:

>Translated_621_residues
MTASKKSSTSAQAGTSTGSIGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQMPSMPDFGA
MASPFAGLKLPAAAIPPERLQALQADYARDCMALMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQE
LADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDMQRGKISQTDESQFVVGKNL
GCTEGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTW
DDYMNEGLLAAIDAVQQVSGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQM
REQTIGGKNGTQPGLMRGVEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRNTYLENK
LREPGALTVCGESVDLTLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNDSD
LPESADDWFAGATEQPGSWWTTWAEWLDQYGGRKVEPPAQPGTAQFPVIEPAPGRYVLQRD
>Mature_620_residues
TASKKSSTSAQAGTSTGSIGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQMPSMPDFGAM
ASPFAGLKLPAAAIPPERLQALQADYARDCMALMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQEL
ADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDMQRGKISQTDESQFVVGKNLG
CTEGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTWD
DYMNEGLLAAIDAVQQVSGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMR
EQTIGGKNGTQPGLMRGVEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRNTYLENKL
REPGALTVCGESVDLTLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNDSDL
PESADDWFAGATEQPGSWWTTWAEWLDQYGGRKVEPPAQPGTAQFPVIEPAPGRYVLQRD

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 68207; Mature: 68075

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASKKSSTSAQAGTSTGSIGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCE
TFQFPTSFPFQMPSMPDFGAMASPFAGLKLPAAAIPPERLQALQADYARDCMALMQQAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQELADALQTDPKTRERIRFTVQ
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDMQRGKISQTDESQFVVGKNL
HHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEEECCC
GCTEGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNG
CCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCEEEEEECCCCHHHHHHHCCC
HQVFLVSWRNADASVAHKTWDDYMNEGLLAAIDAVQQVSGREQINTLGFCVGGTMLATAL
CEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMREQTIGGKNGTQPGLMRGVE
HHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHH
FANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRNTYLENK
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LREPGALTVCGESVDLTLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHI
CCCCCCEEEECCCCEEEEEECCHHEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCE
AGVINPPAKKKRSYWVNDSDLPESADDWFAGATEQPGSWWTTWAEWLDQYGGRKVEPPAQ
EEEECCCHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCEECCCCCC
PGTAQFPVIEPAPGRYVLQRD
CCCCCCCEECCCCCCEEEECC
>Mature Secondary Structure 
TASKKSSTSAQAGTSTGSIGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCE
TFQFPTSFPFQMPSMPDFGAMASPFAGLKLPAAAIPPERLQALQADYARDCMALMQQAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQELADALQTDPKTRERIRFTVQ
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDMQRGKISQTDESQFVVGKNL
HHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEEECCC
GCTEGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNG
CCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCEEEEEECCCCHHHHHHHCCC
HQVFLVSWRNADASVAHKTWDDYMNEGLLAAIDAVQQVSGREQINTLGFCVGGTMLATAL
CEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMREQTIGGKNGTQPGLMRGVE
HHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHH
FANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRNTYLENK
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LREPGALTVCGESVDLTLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHI
CCCCCCEEEECCCCEEEEEECCHHEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCE
AGVINPPAKKKRSYWVNDSDLPESADDWFAGATEQPGSWWTTWAEWLDQYGGRKVEPPAQ
EEEECCCHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCEECCCCCC
PGTAQFPVIEPAPGRYVLQRD
CCCCCCCEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]