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The map label for this gene is rimO

Identifier: 78066551

GI number: 78066551

Start: 2120434

End: 2121795

Strand: Reverse

Name: rimO

Synonym: Bcep18194_A5082

Alternate gene names: 78066551

Gene position: 2121795-2120434 (Counterclockwise)

Preceding gene: 78066555

Following gene: 78066550

Centisome position: 57.44

GC content: 64.54

Gene sequence:

>1362_bases
ATGTCCCAATCCCCTAAAGTAGGGTTCGTATCCCTCGGGTGCCCGAAGGCGCTCGTCGACTCCGAACAGATCATCACGCA
ACTGCGCGCCGAAGGTTATGAAATCTCCGGCACCTACGACGGTGCCGACCTCGTCGTCGTGAACACCTGCGGTTTCATCG
ACGAAGCCGTGCAGGAAAGCCTCGACGCGATCGGCGAAGCGCTGACCGAGAACGGCAAGGTGATCGTCACCGGCTGTCTC
GGCGCCAAGTCGAGCGCCAGCGGCTCGAACCTGATCGAGGAAGTCCACCCGAAGGTGCTCGCCGTCACCGGTCCGCACGC
GGTGGGCGAAGTGATGCAGGCCGTGCATTCGCACCTGCCGAAGCCGCACGATCCGTTCACCGATCTCGTGCCTGCCGCCG
GCATCAAGCTCACGCCGCGCCACTACGCATACCTGAAGATTTCCGAAGGCTGCAACCACCGCTGCACGTTCTGCATCATC
CCGTCGATGCGCGGCGACCTCGTGTCGCGTCCGGTCGCCGAAGTGATGCTGGAAGCGGAGAACCTGTTCAAGTCGGGCGT
GAAGGAACTGCTCGTGATCTCGCAGGACACGAGCGCATACGGCGTCGACGTGAAGTACCGCACGGGCTTCTGGAACGGCA
AGCCGATCAAGACGCGCATGACCGACCTGGTCGCCGCGCTCGGCGAACTCGCCGCGCAGTACGGCGCATGGGTGCGCCTG
CACTACGTGTACCCGTATCCCAGCGTCGACGAAGTGATTCCGCTGATGGCCGAAGGCCCGTTCAAGGGCCACGTGCTGCC
GTACCTCGACGTGCCGTTCCAGCACGCGCACCCGGAAGTGCTGAAGCGCATGAAGCGTCCGGCGAATGCCGAGAAGGTGC
TCGAGCGCGTGCAGAAGTGGCGCGAGATCTGCCCGGACCTGACGATCCGCAGCACGTTCATCGCGGGCTTCCCCGGCGAG
ACGGAAGAGCAGTTCGAAACGCTGCTCGACTTCATCCGCGAAGCGGAACTCGATCGCGTCGGCTGCTTCGCGTATTCGCC
GGTCGAAGGCGCCACCGCGAACGAACTCGACGGCGCGCTGCCGGATGACGTTCGCGAGGAACGCCGCGCACGCTTCATGG
AAGTCGCCGAGGAAGTGTCGGCGAACCGCATCCAGCGCAAGGTCGGCAAGACCCTCAAGGTGCTGATCGACGAAGTCAGC
GCGGAAGGCGGGATCGGCCGTACGGCGGCCGATGCGCCGGAGATCGACGGCGTCGTCTATGTCGAGCCGGCGGCGAAGGC
ATCGAAGCGCTACAAGGTCGGCGATTTCGTGTCCGTGAAGATCACGGGCGCCGACGGCCACGATCTGTGGGGCGAGGTGT
AA

Upstream 100 bases:

>100_bases
CGATCAGGCGCGCCACGGCCGCTTTCCCCCGTTTGTTTTGGCTCGTCGCCCCTAAATCGCTGTAAACTCACGCTTTTGCT
GCCACGCCCCCACAATCCAG

Downstream 100 bases:

>100_bases
GCGATGACCGCGGCATTTCCGGAGATCCTCGCGCTCGGCGAGGCGATGATCGAATTCAACCAGTCGCAGCCGGGCCGGCC
TGAATTCCTGCAGGGCTTCG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCL
GAKSSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFTDLVPAAGIKLTPRHYAYLKISEGCNHRCTFCII
PSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAALGELAAQYGAWVRL
HYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVQKWREICPDLTIRSTFIAGFPGE
TEEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDDVREERRARFMEVAEEVSANRIQRKVGKTLKVLIDEVS
AEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_453_residues
MSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCL
GAKSSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFTDLVPAAGIKLTPRHYAYLKISEGCNHRCTFCII
PSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAALGELAAQYGAWVRL
HYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVQKWREICPDLTIRSTFIAGFPGE
TEEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDDVREERRARFMEVAEEVSANRIQRKVGKTLKVLIDEVS
AEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
>Mature_452_residues
SQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCLG
AKSSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFTDLVPAAGIKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAALGELAAQYGAWVRLH
YVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVQKWREICPDLTIRSTFIAGFPGET
EEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGALPDDVREERRARFMEVAEEVSANRIQRKVGKTLKVLIDEVSA
EGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=25.8196721311475, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872784, Length=493, Percent_Identity=25.5578093306288, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI93277076, Length=414, Percent_Identity=27.0531400966184, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=64.1592920353982, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI1786882, Length=464, Percent_Identity=25.8620689655172, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=489, Percent_Identity=24.9488752556237, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=26.8211920529801, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=28.659793814433, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922432, Length=461, Percent_Identity=26.4642082429501, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49448; Mature: 49317

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQES
CCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCHHHHHHHHH
LDAIGEALTENGKVIVTGCLGAKSSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLP
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCC
KPHDPFTDLVPAAGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVAEVMLEAE
CCCCCHHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHH
NLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAALGELAAQYGAWVRL
HHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
HYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVQKW
EEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REICPDLTIRSTFIAGFPGETEEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGAL
HHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCCCCCHHHCCCCC
PDDVREERRARFMEVAEEVSANRIQRKVGKTLKVLIDEVSAEGGIGRTAADAPEIDGVVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
ECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQES
CCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCHHHHHHHHH
LDAIGEALTENGKVIVTGCLGAKSSASGSNLIEEVHPKVLAVTGPHAVGEVMQAVHSHLP
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCC
KPHDPFTDLVPAAGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVAEVMLEAE
CCCCCHHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHH
NLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAALGELAAQYGAWVRL
HHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
HYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVLERVQKW
EEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REICPDLTIRSTFIAGFPGETEEQFETLLDFIREAELDRVGCFAYSPVEGATANELDGAL
HHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCCCCCHHHCCCCC
PDDVREERRARFMEVAEEVSANRIQRKVGKTLKVLIDEVSAEGGIGRTAADAPEIDGVVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VEPAAKASKRYKVGDFVSVKITGADGHDLWGEV
ECCCHHCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA