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The map label for this gene is ycaN [H]

Identifier: 78065829

GI number: 78065829

Start: 1312557

End: 1313531

Strand: Reverse

Name: ycaN [H]

Synonym: Bcep18194_A4358

Alternate gene names: 78065829

Gene position: 1313531-1312557 (Counterclockwise)

Preceding gene: 78065835

Following gene: 78065827

Centisome position: 35.56

GC content: 66.87

Gene sequence:

>975_bases
ATGAACTCTGCTCATCAATTGAAAACCGGCGATCTCTTCTCGCTCAACGTGTTCCTCGCCGCGGCCACGCACCTCAGCTT
TCGCGCGGCATCGATCGAGCTCGACGTCACGCCGTCGGCAATCAGTCATTCGATCAAGAGCCTCGAGCAGCGCCTGGGCG
TTCGCCTGTTCAATCGCACCACGCGCAGCGTGTCGTTGACGGACGCCGGCGAGCGGCTCGTCGACAAGCTCCGCCCGGCG
ATCTCGTCGGTTGCGGAGGCGATGCTCGCGGTCGACGGGATGCGCGACACGCCCAGCGGCACCGTGCGCATCAACGCGAG
CGAAGGGGCGATCCGCCTCGTGCTGAAGCCGGTGCTCGCGCGCTTCCTGCGCGACTACCCGCAGGTCCACCTCGACATCG
TGACGGACGGCAGGCTCGGCGACATCGTCGCCGGTGGATTCGACGCGGGGATCCGGCTGGCCGAAGCCGTGCCGCAGGAC
ATGATCGCGGTGCGCGTGTCCGATCCGGTGCGGTTCGCCGCCGTCGCGTCGCCCGCTTATTTCGCCGCGCATGGCCGCCC
CGACGTACCGCAGGATCTGGAGCGGCACGCCTGCATCCGCTTTCGCTTCGATAGCGGTGCGATCTACCGCTGGGAGTTCG
AGCGCCGCGGTGTCGTCGAAACGGTGAACGTCAGCGGCCCGCTCACGCTGACCGACCAGCCGCTGATGGTCGATGCCGCG
ATCGACGGCATCGGAATTGCGTTCGTGCCCGATCATCTCGTTCACGATGCGCTGGCCGATGGGCGTCTCGAACGCGTGCT
GGCCGACTGGTGTCCGGTCATGCCGGGGCTGTGCCTGTATTACCCCGGTCATCGCCATGTTTCCGGAGGGCTGCGGGCGC
TGATCGACAGGGTTCGGATTCCGTCGCCCGATGCCAATCGAGCGCCTTCGCAACCGGCACGGCGCCGCAAAGGCAGGAAA
CCCGCGAGCGATTGA

Upstream 100 bases:

>100_bases
TCGCCTTCATCTTCATTGCCTGCTCCTTGTTCAACCGTACGCAGGGCCTAGTCTAGAAAAGGCGCATTATCATGACAATC
TCGCCAATCTTTACGTGAAG

Downstream 100 bases:

>100_bases
TCGGGTCGATGATGGCGGTGCCGGCACACAGCCCACCGCGCTGCTTGCATCGCATGCCGACGATCGGCTGCAACGATCAG
CACGCACACCTAACGATGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNSAHQLKTGDLFSLNVFLAAATHLSFRAASIELDVTPSAISHSIKSLEQRLGVRLFNRTTRSVSLTDAGERLVDKLRPA
ISSVAEAMLAVDGMRDTPSGTVRINASEGAIRLVLKPVLARFLRDYPQVHLDIVTDGRLGDIVAGGFDAGIRLAEAVPQD
MIAVRVSDPVRFAAVASPAYFAAHGRPDVPQDLERHACIRFRFDSGAIYRWEFERRGVVETVNVSGPLTLTDQPLMVDAA
IDGIGIAFVPDHLVHDALADGRLERVLADWCPVMPGLCLYYPGHRHVSGGLRALIDRVRIPSPDANRAPSQPARRRKGRK
PASD

Sequences:

>Translated_324_residues
MNSAHQLKTGDLFSLNVFLAAATHLSFRAASIELDVTPSAISHSIKSLEQRLGVRLFNRTTRSVSLTDAGERLVDKLRPA
ISSVAEAMLAVDGMRDTPSGTVRINASEGAIRLVLKPVLARFLRDYPQVHLDIVTDGRLGDIVAGGFDAGIRLAEAVPQD
MIAVRVSDPVRFAAVASPAYFAAHGRPDVPQDLERHACIRFRFDSGAIYRWEFERRGVVETVNVSGPLTLTDQPLMVDAA
IDGIGIAFVPDHLVHDALADGRLERVLADWCPVMPGLCLYYPGHRHVSGGLRALIDRVRIPSPDANRAPSQPARRRKGRK
PASD
>Mature_324_residues
MNSAHQLKTGDLFSLNVFLAAATHLSFRAASIELDVTPSAISHSIKSLEQRLGVRLFNRTTRSVSLTDAGERLVDKLRPA
ISSVAEAMLAVDGMRDTPSGTVRINASEGAIRLVLKPVLARFLRDYPQVHLDIVTDGRLGDIVAGGFDAGIRLAEAVPQD
MIAVRVSDPVRFAAVASPAYFAAHGRPDVPQDLERHACIRFRFDSGAIYRWEFERRGVVETVNVSGPLTLTDQPLMVDAA
IDGIGIAFVPDHLVHDALADGRLERVLADWCPVMPGLCLYYPGHRHVSGGLRALIDRVRIPSPDANRAPSQPARRRKGRK
PASD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=44.5229681978799, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=36.734693877551, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=30.8771929824561, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=31.6602316602317, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=283, Percent_Identity=30.7420494699647, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789639, Length=258, Percent_Identity=29.4573643410853, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789440, Length=256, Percent_Identity=27.34375, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=27.9693486590038, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=26.8041237113402, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787879, Length=166, Percent_Identity=33.7349397590361, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=154, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693105, Length=146, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=27.4900398406374, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=35.8333333333333, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790011, Length=269, Percent_Identity=26.0223048327138, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35286; Mature: 35286

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAHQLKTGDLFSLNVFLAAATHLSFRAASIELDVTPSAISHSIKSLEQRLGVRLFNRT
CCCCCCCCCCCEEEHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHCHHHHCCC
TRSVSLTDAGERLVDKLRPAISSVAEAMLAVDGMRDTPSGTVRINASEGAIRLVLKPVLA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHH
RFLRDYPQVHLDIVTDGRLGDIVAGGFDAGIRLAEAVPQDMIAVRVSDPVRFAAVASPAY
HHHHCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCE
FAAHGRPDVPQDLERHACIRFRFDSGAIYRWEFERRGVVETVNVSGPLTLTDQPLMVDAA
EEECCCCCCHHHHHCCEEEEEEECCCCEEEEEECCCCCEEEEECCCCEEECCCCEEEEEE
IDGIGIAFVPDHLVHDALADGRLERVLADWCPVMPGLCLYYPGHRHVSGGLRALIDRVRI
CCCCEEEECCHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCC
PSPDANRAPSQPARRRKGRKPASD
CCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MNSAHQLKTGDLFSLNVFLAAATHLSFRAASIELDVTPSAISHSIKSLEQRLGVRLFNRT
CCCCCCCCCCCEEEHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHCHHHHCCC
TRSVSLTDAGERLVDKLRPAISSVAEAMLAVDGMRDTPSGTVRINASEGAIRLVLKPVLA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHH
RFLRDYPQVHLDIVTDGRLGDIVAGGFDAGIRLAEAVPQDMIAVRVSDPVRFAAVASPAY
HHHHCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCE
FAAHGRPDVPQDLERHACIRFRFDSGAIYRWEFERRGVVETVNVSGPLTLTDQPLMVDAA
EEECCCCCCHHHHHCCEEEEEEECCCCEEEEEECCCCCEEEEECCCCEEECCCCEEEEEE
IDGIGIAFVPDHLVHDALADGRLERVLADWCPVMPGLCLYYPGHRHVSGGLRALIDRVRI
CCCCEEEECCHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCC
PSPDANRAPSQPARRRKGRKPASD
CCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]