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The map label for this gene is amtB [C]

Identifier: 78065781

GI number: 78065781

Start: 1256821

End: 1258014

Strand: Reverse

Name: amtB [C]

Synonym: Bcep18194_A4310

Alternate gene names: 78065781

Gene position: 1258014-1256821 (Counterclockwise)

Preceding gene: 78065782

Following gene: 78065780

Centisome position: 34.05

GC content: 67.84

Gene sequence:

>1194_bases
ATGGATGGTCTGAAATCCGGCGTCGACACACTGTTCCTGTTGATCGGCGCCGTCATGGTGCTCGCGATGCACGCGGGCTT
TGCATTTCTCGAACTCGGCACGGTCCGCAAGAAGAACCAGGTCAACGCGCTCGTGAAGATCCTGGTCGACTTCTCGGTGT
CGACACTCGCGTACTTCTTCATCGGGTACACGATCGCGTACGGCGTCGAGTTCTTCGACGACATCGGCACGCTGTCGCAG
CACAGCGGCTACGCGCTCGTGCGCTTCTTCTTCCTGCTGACCTTCGCGGCGGCGATTCCCGCGATCGTGTCCGGCGGCAT
CGCCGAGCGCGCGAAGTTCAATCCGCAGCTCGTCGCGACGCTGATCATCGTCGGCTTCATCTATCCGTTCTTCGAAGGAA
TCGCCTGGAACAACCGCTTCGGCGTGCAGGCCTGGCTCACGCACGCGTTCGGTGCGCCGTTCCATGATTTCGCGGGCTCC
GTCGTCGTGCACGCGTTCGGCGGCTGGGTCGCGCTGCCGGCCGTGCTGCTGCTCGGCGCGCGCCACGGCCGCTATGCGAA
GGACGGCCGGATCGCCGCGCACCCGCCGTCGAACATCCCGTTCCTCGCGCTCGGCGCGTGGGTACTCGCGGTCGGCTGGT
TCGGCTTCAACGTGATGAGTGCGCAGACGCTCGACAAGATCAGCGGCCTCGTCGCCGTGAACTCGCTGATGGCGATGGTC
GGCGGCACGCTCGCCGCGTGGATGGCCGGCCGCAACGACCCGGGCTTCACGTACAACGGGCCGCTCGCGGGCCTCGTCGC
GGTGTGCGCGGGCTCCGACGTGATGCACCCGATCGGCGCACTGGTCACGGGTGCCGCGGCCGGCGCACTGTTCGTCGCGA
TGTTCACCTGCGTGCAGAACAAGTGGCGCATCGACGACGTGCTCGGCGTGTGGCCGCTGCACGGCATGTGCGGCGCGCTC
GGCGGCCTTGCTGCCGGCGTGTTCGGCCTGCCGGCGCTCGGCGGTCTCGGCGGCGTGTCGTTCATGTCGCAGCTCGTCGG
CACGCTCGGCGGCATCGCGATCGCGACCGCGGGCGGCACGCTCGTCTACGGCGCGCTGAAAGCGACCGTCGGGCTGCGCC
TCGATCGCGAAGCCGAGTTCGACGGCGCGGACCTGTCGATCCACCGCATCTCGGCAACGCCCGAACGCGATTGA

Upstream 100 bases:

>100_bases
CGCACCACACGGTGTCGAAGCACCAACTCCACGCAGCCGTTCACCAAGTTGGCTCGATACTTGCGTTCCTTGCCCGTTTT
TGGCGCAAGGAAGCCACGGC

Downstream 100 bases:

>100_bases
GCGCCTGACGAACGCCCGACGTATCATTACGTTTTCAATTCAAAAAACTTTCATCTAGCGTCCTTACACTTCCGTCCAGT
TTTCAATTCGCCTTCGCCGA

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNNRFGVQAWLTHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFMSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD

Sequences:

>Translated_397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNNRFGVQAWLTHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFMSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD
>Mature_397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNNRFGVQAWLTHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFMSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=30.8641975308642, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=29.5612009237875, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=28.8329519450801, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=26.8518518518519, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=27.7904328018223, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=24.1545893719807, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=23.6714975845411, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324187, Length=407, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=28.7081339712919, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41488; Mature: 41488

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]