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The map label for this gene is bcr [H]

Identifier: 78065539

GI number: 78065539

Start: 992268

End: 993485

Strand: Direct

Name: bcr [H]

Synonym: Bcep18194_A4067

Alternate gene names: 78065539

Gene position: 992268-993485 (Clockwise)

Preceding gene: 78065534

Following gene: 78065543

Centisome position: 26.86

GC content: 69.95

Gene sequence:

>1218_bases
ATGTCTCACGTCATCCGACGCCGGCCCGATGCCCGGCTGATCCTGTTGCTCGGCGCGCTCGCCGCCTGCGGGCCGATCGC
TACCGACATGTACCTGCCGAGCCTGCCGTCGATCGCCGACGGCTTCTCCGTCAGCCCCGGCGCCGCGCAGCGCACGCTGA
CGAGCTTCATGGCCGGCTTCTCGGTCGGCATGCTGCTGTACGGCCCGCTGTCCGACACGTGGGGCCGACGCCCCGTGCTG
CTCGGCGGCATCGCGCTGTTCACGCTCGCGAGCATCGGCTGCTTCGTGTCGACTTCGATCGACATGCTGATCATCGTGCG
CTTCCTGCAGGCACTTGGCGCCGGCGCGGCGTCGGTGCTGTCGCGAGCGATCGCGCGCGACGCGCACGAGCCGACCGACG
CCGCGAAGGTGCTGTCGATGGTCGCGATCGTCACCGCGGTGGGGCCGTTGCTTGCGCCGCTGATCGGCGGGCAGATCCTG
CGCTTCTCCGGTTGGCGCGGCGTGTTCGTCGTGCTGGCCGTGTTCGGCGCGGTGTGCGCGGCGACCGCGTACCTGCGCGT
GCCCGAAACCTGGCCGAAGGAGCGGCGCAAGAGCGCGGCCGTGCTCGCGTCGTTCGCGTCGTACGGGCGCATCCTGTCCG
ACCCCGTTGCCTGGGGCCACATGCTGTGCGGCGGGATGGCATTTGCGTCGATGTTCTCGTACATCACCGCCACGCCATTC
GTGTACATCGAGTATTTCCACGTGTCGCCGCAACACTACGGGCTGCTGTTCGGCCTGAACGTCGTCGGCATCATGATCGG
CAACTTCGCGAACACGCGGCTCGTCGGCCGCATCGGTTCGCTGCGCATCATCGCGGCCGCGTCGCTCGTGAGCTGCATCG
CGTCGCTCGCGGTCGCGCTCGTCGCGCTGACGGGGTGGGGCGGGCTGTGGTCGATCGTCGTGTGCCTGTTCTTCGTGGTC
GGCGTGGTCGGGATCCTGTCCGCGAACTGCACGACCGACCTCATGCATCGCTATCCGCACAACGCGGGCGCGTCGGCGGC
CGTGTTCGGCGCGATGCAGCTGGCGCTCGGCGCGCTCGCGAGCGTCGCGATCGGCGCACTCGCGGACGGCACGCCGTTCG
CGATGGGCGTGACGATCGGCATCTCGGGCGTGCTGTGTTTCGCCGGCCGCTACCTCGTGCTGCGCTGGCACGGGCGGCCC
GTCAAGGCGGGCGCCTGA

Upstream 100 bases:

>100_bases
GCGCGTACAATCATGGGCCTGGGCGCACCGTTCGCGGCGCGGCCTCCCGTCTTCACCGCCACGCGCGCCCCGATGCGCAC
GGGCTCCATTCCGGATACCC

Downstream 100 bases:

>100_bases
ACCGCGGCCTGACGGCAAAATGAAAAACGCCACCGCGCGCGCTGCGCCGGTGGCGTTTTTGTTTCGAACGCGGGAAGCGG
CTGCCGCCGCACCGCGTCAA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSHVIRRRPDARLILLLGALAACGPIATDMYLPSLPSIADGFSVSPGAAQRTLTSFMAGFSVGMLLYGPLSDTWGRRPVL
LGGIALFTLASIGCFVSTSIDMLIIVRFLQALGAGAASVLSRAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFSGWRGVFVVLAVFGAVCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANTRLVGRIGSLRIIAAASLVSCIASLAVALVALTGWGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGISGVLCFAGRYLVLRWHGRP
VKAGA

Sequences:

>Translated_405_residues
MSHVIRRRPDARLILLLGALAACGPIATDMYLPSLPSIADGFSVSPGAAQRTLTSFMAGFSVGMLLYGPLSDTWGRRPVL
LGGIALFTLASIGCFVSTSIDMLIIVRFLQALGAGAASVLSRAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFSGWRGVFVVLAVFGAVCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANTRLVGRIGSLRIIAAASLVSCIASLAVALVALTGWGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGISGVLCFAGRYLVLRWHGRP
VKAGA
>Mature_404_residues
SHVIRRRPDARLILLLGALAACGPIATDMYLPSLPSIADGFSVSPGAAQRTLTSFMAGFSVGMLLYGPLSDTWGRRPVLL
GGIALFTLASIGCFVSTSIDMLIIVRFLQALGAGAASVLSRAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILR
FSGWRGVFVVLAVFGAVCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPFV
YIEYFHVSPQHYGLLFGLNVVGIMIGNFANTRLVGRIGSLRIIAAASLVSCIASLAVALVALTGWGGLWSIVVCLFFVVG
VVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGALADGTPFAMGVTIGISGVLCFAGRYLVLRWHGRPV
KAGA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=397, Percent_Identity=34.2569269521411, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI48994889, Length=396, Percent_Identity=28.030303030303, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082312, Length=365, Percent_Identity=29.041095890411, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=202, Percent_Identity=33.1683168316832, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787065, Length=257, Percent_Identity=27.2373540856031, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790794, Length=223, Percent_Identity=26.0089686098655, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790195, Length=163, Percent_Identity=28.2208588957055, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=178, Percent_Identity=24.1573033707865, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=165, Percent_Identity=28.4848484848485, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42222; Mature: 42090

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVIRRRPDARLILLLGALAACGPIATDMYLPSLPSIADGFSVSPGAAQRTLTSFMAGF
CCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SVGMLLYGPLSDTWGRRPVLLGGIALFTLASIGCFVSTSIDMLIIVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SRAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILRFSGWRGVFVVLAVFGAVCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
ATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANTRLVGRIGSLRIIAAASLVSCIASLAVAL
EEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VALTGWGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGALADGTPFAMGVTIGISGVLCFAGRYLVLRWHGRPVKAGA
HHHHHHHCCCCCHHEEHHHHHHHHHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SHVIRRRPDARLILLLGALAACGPIATDMYLPSLPSIADGFSVSPGAAQRTLTSFMAGF
CCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SVGMLLYGPLSDTWGRRPVLLGGIALFTLASIGCFVSTSIDMLIIVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SRAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILRFSGWRGVFVVLAVFGAVCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
ATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFANTRLVGRIGSLRIIAAASLVSCIASLAVAL
EEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VALTGWGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGALADGTPFAMGVTIGISGVLCFAGRYLVLRWHGRPVKAGA
HHHHHHHCCCCCHHEEHHHHHHHHHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]