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The map label for this gene is trmB

Identifier: 78065494

GI number: 78065494

Start: 946551

End: 947318

Strand: Reverse

Name: trmB

Synonym: Bcep18194_A4022

Alternate gene names: 78065494

Gene position: 947318-946551 (Counterclockwise)

Preceding gene: 78065498

Following gene: 78065490

Centisome position: 25.64

GC content: 68.62

Gene sequence:

>768_bases
ATGATGCACGACGATCCGAACGAAGCCGGCCTGCCGCCGCACGACGACGCCATTCCCGACGAAGCCGCCGAAGGCGCCGA
CGCAGTCAATCCGCTGCACCACCGCCGCATCCGCAGCTTCGTGACGCGCGCCGGCCGTGTGTCGACTGGCCAGCGCCGCG
CGATGGACGAACTCGGCCCGCGCTTCGTGGTGCCGTATGCGCCGGAGCTGCCCGACTGGAACGCGGTGTTCGGCCGCAGT
GCACCGCGCATCCTCGAGATCGGCTTCGGGATGGGCGCATCGACCGCGGAAATCGCCGCGAACCGCCCGGGTGACGACTT
CCTCGGCGTCGAAGTGCACGAACCGGGCGTCGGCGCGCTGCTGAAGCTGATCGGCGAGCAGGACCTGTCGAACATCCGCA
TCATCCAGCACGACGCGGTCGAAGTGCTCGAGCACATGCTCGCACCGGAAAGCCTCGACGGCGTGCACATCTTCTTCCCC
GATCCGTGGCACAAGGCGCGCCACCACAAGCGCCGGCTGATCCAGCCGCCGCTCGTCGCGCATCTCGTATCGCGCCTGAA
GCCCGGTGCGTACATTCACTGCGCGACCGACTGGCAGAACTATGCGGAGCAGATGCTCGAAGTGCTCGGCGCGGAGCCGA
CGCTCGAAAACACGGCCGCCGACTACGCACCGCGCCCCGACTACCGCCCGGTGACGAAGTTCGAACGGCGCGGGCTGCGG
CTCGGCCACGGCGTGTGGGACCTGGTGTTCCGTAAGCGCGCAGCCTGA

Upstream 100 bases:

>100_bases
CCTCGTCGAAGGCGGCTTTTTTGTACCATGTGCCGGTTCCCCCGGCCCACTGCTGCGCGCAACGGGCCCTCGCCCGCAAC
CGAGCCTTTCTCTGTCACCG

Downstream 100 bases:

>100_bases
GTTCTCCTGCTGCGGCAGCGCACGTCGCTCCGCTCCGGACAAGAAAAAGCCCGACCGGCCTATGCCGATCGGGCTTTTTT
CATGCGAGGCGCGCCCGCCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MMHDDPNEAGLPPHDDAIPDEAAEGADAVNPLHHRRIRSFVTRAGRVSTGQRRAMDELGPRFVVPYAPELPDWNAVFGRS
APRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDAVEVLEHMLAPESLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLVSRLKPGAYIHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAA

Sequences:

>Translated_255_residues
MMHDDPNEAGLPPHDDAIPDEAAEGADAVNPLHHRRIRSFVTRAGRVSTGQRRAMDELGPRFVVPYAPELPDWNAVFGRS
APRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDAVEVLEHMLAPESLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLVSRLKPGAYIHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAA
>Mature_255_residues
MMHDDPNEAGLPPHDDAIPDEAAEGADAVNPLHHRRIRSFVTRAGRVSTGQRRAMDELGPRFVVPYAPELPDWNAVFGRS
APRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDAVEVLEHMLAPESLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLVSRLKPGAYIHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=220, Percent_Identity=54.5454545454545, Blast_Score=242, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 28440; Mature: 28440

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHDDPNEAGLPPHDDAIPDEAAEGADAVNPLHHRRIRSFVTRAGRVSTGQRRAMDELGP
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
RFVVPYAPELPDWNAVFGRSAPRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGAL
EEEECCCCCCCCCHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHH
LKLIGEQDLSNIRIIQHDAVEVLEHMLAPESLDGVHIFFPDPWHKARHHKRRLIQPPLVA
HHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHH
HLVSRLKPGAYIHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
HHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCC
LGHGVWDLVFRKRAA
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MMHDDPNEAGLPPHDDAIPDEAAEGADAVNPLHHRRIRSFVTRAGRVSTGQRRAMDELGP
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
RFVVPYAPELPDWNAVFGRSAPRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGAL
EEEECCCCCCCCCHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHH
LKLIGEQDLSNIRIIQHDAVEVLEHMLAPESLDGVHIFFPDPWHKARHHKRRLIQPPLVA
HHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHH
HLVSRLKPGAYIHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
HHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCC
LGHGVWDLVFRKRAA
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA