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The map label for this gene is waaA [H]

Identifier: 78065474

GI number: 78065474

Start: 928304

End: 929650

Strand: Direct

Name: waaA [H]

Synonym: Bcep18194_A4002

Alternate gene names: 78065474

Gene position: 928304-929650 (Clockwise)

Preceding gene: 78065473

Following gene: 78065491

Centisome position: 25.13

GC content: 72.09

Gene sequence:

>1347_bases
ATGCTGAGGGTGATCTATCGCGCGCTGTGGTGGCTCGTCGCACCGATCGCCGTCATCCGGCTTTACGTGCGCTCGCGCAA
GGAGCGTGGCTATCGCGAGCACATCGGCGAACGTTTCGGCCACGTGGCCGGCCGCTCGCGCGACGACCGCGCGCCGCTGA
TCTGGGTGCATGCGGTATCCGTCGGCGAGACCCGCGCGGCGCAGCCGCTGATCGACGCGCTGATGCATGCCCGCCCCGAT
GCGCGCATCCTGCTCACGCACATGACGCCGAGCGGCCGCGCGACCGGCGAACAGATCTTCGGCGATCGCGTGCTGCGCTG
CTATCTGCCGTACGACATGCCCGGCGCGGTACGACGCTTCCTGCGCGCGTGGCGGCCGACGCTCGGCCTCGTGATGGAAA
CCGAGGTGTGGCCCACGCTGATCGACGAGTGCCGCCGCGCGGACGTGCCGCTGGTGCTGACCAATGCGCGCATGTCCGCG
CGTTCGTTCCGGCGTGCATCGAAGTTCGGCGCGGCGACGCACGACGTGTTCGGCGGCTTCTCGCGCGTGCTCGCGCAGAG
CCCGGCCGATGCGGAGCGGCTGTCGTCGCTCGGCGCGCGCAACGTGACCGTGCTCGGCAACCTGAAGTTCGACATGACGA
CGCCGCCGGAACTCGCGGCGCGTGGCCATGCGTGGCGCGAGGCGATCGGCGCGCGGCCCGTGTGGGTGGCCGCGAGCACG
CGCGAGAACGAGGAGGCGCTGGTGCTCGACGCATTCACGGCGATGCGCACGCCCGGCGCGCTGCTGGTGCTCGTACCGCG
TCATCCGCAGCGCTTCGCCGAGGTCGAGGCGCTCGTCGAGCGCAGCGGGCTCAAGTGCGTGCGACGCTCGGTGTGGGCGG
CCGACAAGGCCGCGCTCGCGGCCGGACGGCCGGCTGCCGAGCCGCTGCCGGACGACGTATCGGTGTTGCTCGGCGATTCG
ATGGGCGAGCTCGGCGCGTATTACGCGGCGGCCGACATCGCGTTCATCGGCGGCAGCCTGTTGCCGCTCGGCGGCCAGAA
CCTGATCGAGGCGTGCGCGGTTGGCGTGCCGGTGCTGATCGGCAAGCACGTGTTCAACTTCACGCAGGCGACCGCCGATG
CGGTCGCGGCCGGCGCGGCGCTGCAGGTCGAGGATCCGCTCGATCTCGCGCACGTGCTCGACGCGCTGTTCGCCGACAAG
GCGCGGCGCATCGCGATGGGCGCGGCCGGCGCCGCATTCGCGTCGCGTCACCGCGGCGCGACCGCACGCACGGTCGACGT
GCTCGCGGCACTGCTGCCGCCCGCCGAGCGTGGCGTGCGCACGCTGCAGGACGCTTCCGACGACTAG

Upstream 100 bases:

>100_bases
GTGCGCCGCGAATGCGCGCCGCTCGGCATTCTCGAACGCCTGAAGGGCAGGGCGCTGGTTTGAGCGCGCCTCTTCTCCGT
TGCAACATGAGGGTCGCCGC

Downstream 100 bases:

>100_bases
GCGAAGGCGGCGCAATGCCGCCATGAGCACGCGTGGCGGCACCGTATGGCGCCGCCACGCCGCTCAGACCGTCAGCAACC
CCTTCTTCTCGATGAACGCG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MLRVIYRALWWLVAPIAVIRLYVRSRKERGYREHIGERFGHVAGRSRDDRAPLIWVHAVSVGETRAAQPLIDALMHARPD
ARILLTHMTPSGRATGEQIFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSFRRASKFGAATHDVFGGFSRVLAQSPADAERLSSLGARNVTVLGNLKFDMTTPPELAARGHAWREAIGARPVWVAAST
RENEEALVLDAFTAMRTPGALLVLVPRHPQRFAEVEALVERSGLKCVRRSVWAADKAALAAGRPAAEPLPDDVSVLLGDS
MGELGAYYAAADIAFIGGSLLPLGGQNLIEACAVGVPVLIGKHVFNFTQATADAVAAGAALQVEDPLDLAHVLDALFADK
ARRIAMGAAGAAFASRHRGATARTVDVLAALLPPAERGVRTLQDASDD

Sequences:

>Translated_448_residues
MLRVIYRALWWLVAPIAVIRLYVRSRKERGYREHIGERFGHVAGRSRDDRAPLIWVHAVSVGETRAAQPLIDALMHARPD
ARILLTHMTPSGRATGEQIFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSFRRASKFGAATHDVFGGFSRVLAQSPADAERLSSLGARNVTVLGNLKFDMTTPPELAARGHAWREAIGARPVWVAAST
RENEEALVLDAFTAMRTPGALLVLVPRHPQRFAEVEALVERSGLKCVRRSVWAADKAALAAGRPAAEPLPDDVSVLLGDS
MGELGAYYAAADIAFIGGSLLPLGGQNLIEACAVGVPVLIGKHVFNFTQATADAVAAGAALQVEDPLDLAHVLDALFADK
ARRIAMGAAGAAFASRHRGATARTVDVLAALLPPAERGVRTLQDASDD
>Mature_448_residues
MLRVIYRALWWLVAPIAVIRLYVRSRKERGYREHIGERFGHVAGRSRDDRAPLIWVHAVSVGETRAAQPLIDALMHARPD
ARILLTHMTPSGRATGEQIFGDRVLRCYLPYDMPGAVRRFLRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSFRRASKFGAATHDVFGGFSRVLAQSPADAERLSSLGARNVTVLGNLKFDMTTPPELAARGHAWREAIGARPVWVAAST
RENEEALVLDAFTAMRTPGALLVLVPRHPQRFAEVEALVERSGLKCVRRSVWAADKAALAAGRPAAEPLPDDVSVLLGDS
MGELGAYYAAADIAFIGGSLLPLGGQNLIEACAVGVPVLIGKHVFNFTQATADAVAAGAALQVEDPLDLAHVLDALFADK
ARRIAMGAAGAAFASRHRGATARTVDVLAALLPPAERGVRTLQDASDD

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=443, Percent_Identity=40.8577878103837, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 48511; Mature: 48511

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVIYRALWWLVAPIAVIRLYVRSRKERGYREHIGERFGHVAGRSRDDRAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
VGETRAAQPLIDALMHARPDARILLTHMTPSGRATGEQIFGDRVLRCYLPYDMPGAVRRF
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHH
LRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSFRRASKFGAATHDVFGGF
HHHHCCHHCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
SRVLAQSPADAERLSSLGARNVTVLGNLKFDMTTPPELAARGHAWREAIGARPVWVAAST
HHHHHCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHCCCCEEEEECC
RENEEALVLDAFTAMRTPGALLVLVPRHPQRFAEVEALVERSGLKCVRRSVWAADKAALA
CCCCCEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRPAAEPLPDDVSVLLGDSMGELGAYYAAADIAFIGGSLLPLGGQNLIEACAVGVPVLI
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHH
GKHVFNFTQATADAVAAGAALQVEDPLDLAHVLDALFADKARRIAMGAAGAAFASRHRGA
HHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
TARTVDVLAALLPPAERGVRTLQDASDD
HHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRVIYRALWWLVAPIAVIRLYVRSRKERGYREHIGERFGHVAGRSRDDRAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
VGETRAAQPLIDALMHARPDARILLTHMTPSGRATGEQIFGDRVLRCYLPYDMPGAVRRF
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHH
LRAWRPTLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSFRRASKFGAATHDVFGGF
HHHHCCHHCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
SRVLAQSPADAERLSSLGARNVTVLGNLKFDMTTPPELAARGHAWREAIGARPVWVAAST
HHHHHCCCCCHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHCCCCEEEEECC
RENEEALVLDAFTAMRTPGALLVLVPRHPQRFAEVEALVERSGLKCVRRSVWAADKAALA
CCCCCEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRPAAEPLPDDVSVLLGDSMGELGAYYAAADIAFIGGSLLPLGGQNLIEACAVGVPVLI
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHH
GKHVFNFTQATADAVAAGAALQVEDPLDLAHVLDALFADKARRIAMGAAGAAFASRHRGA
HHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
TARTVDVLAALLPPAERGVRTLQDASDD
HHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]