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The map label for this gene is argT [H]

Identifier: 78065413

GI number: 78065413

Start: 859898

End: 860677

Strand: Direct

Name: argT [H]

Synonym: Bcep18194_A3939

Alternate gene names: 78065413

Gene position: 859898-860677 (Clockwise)

Preceding gene: 78065407

Following gene: 78065414

Centisome position: 23.28

GC content: 65.38

Gene sequence:

>780_bases
ATGAACTGGAAGCTCTCCCTTTGCGCCGCTGCGGCGCTTGCCTGCGCGGCCGTCACGGCCCATGCGGAACAGACCACGTT
GCGCTTCGGGATCGAAGCCGCCTATCCGCCGTTCGAGAGCAAGACGCCGGCCGGCCAGTTGCAGGGTTTCGACGTCGACA
TCGGCAATGCGGTGTGCGCGAAGCTGAACATGAAATGCGTGTGGGTCGAGAATTCGTTCGACGGCCTGATCCCGGCGCTG
CAGGCGCGCAAGTTCGACGCGATCAACTCGGCGATGAACATCACGTCGAAGCGCCGGCAGAGCATCGACTTCACGCCGGC
GATCTACGTGGTGCCGATCGTGATGATCGCCAAGCACGGCTCGCCGCTGCGGCCCGATGTCGCGAGCCTGCGCGGCAAGC
ATGTCGGCGTGCTGCAGGGCTCGTCGCAGGAGGATTTCCTGAAGGCACACTGGGCCACTGCGGGCGTGGCCGTCGTGTCG
TACCAGGATCAGGACCAGATCTACGCCGATCTCGTCGCGGGGCGTCTCGACGCGGCCGTGCAGGAAGCGCAGACCGCGCA
GGACGGTTTCCTCGACAAGCCGGCCGGCCGCGACTACCAGATCGTCGGCGAGCCGCTGAAGGATCCGGCGACGCTCGGCG
AAGGCACGGGCTTCGGGATGCGCAAGAACGACAAGGCGCTGCAGGCGAAGATCGTCGGCGCGCTCGATGCGCTGAAGAAG
GACGGCACACTCAGCGCGCTGTCGCAGAAGTACTTCAAGCGCGACATCGTTTCGAAGTAG

Upstream 100 bases:

>100_bases
CGCCGCGTGGCGGCCGCCTGCTTCGAATGGCGATCCGGCGCCCGGCCGGGGCCGTCGTGTACCGATCTTCATCGCCTCAA
TCCGGAACGGAAGGACCGTC

Downstream 100 bases:

>100_bases
TCACCGCCGTGCGCCGGCCGCCGCGCCGGCGCGTGTCGCATCGAATCTCGACGCAGTACGGAGAACCATGGATTTCGACG
TCATCGTTCTGGGGGCCGGC

Product: ABC amino acid transporter, periplasmic ligand binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNWKLSLCAAAALACAAVTAHAEQTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENSFDGLIPAL
QARKFDAINSAMNITSKRRQSIDFTPAIYVVPIVMIAKHGSPLRPDVASLRGKHVGVLQGSSQEDFLKAHWATAGVAVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKNDKALQAKIVGALDALKK
DGTLSALSQKYFKRDIVSK

Sequences:

>Translated_259_residues
MNWKLSLCAAAALACAAVTAHAEQTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENSFDGLIPAL
QARKFDAINSAMNITSKRRQSIDFTPAIYVVPIVMIAKHGSPLRPDVASLRGKHVGVLQGSSQEDFLKAHWATAGVAVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKNDKALQAKIVGALDALKK
DGTLSALSQKYFKRDIVSK
>Mature_259_residues
MNWKLSLCAAAALACAAVTAHAEQTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCAKLNMKCVWVENSFDGLIPAL
QARKFDAINSAMNITSKRRQSIDFTPAIYVVPIVMIAKHGSPLRPDVASLRGKHVGVLQGSSQEDFLKAHWATAGVAVVS
YQDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKNDKALQAKIVGALDALKK
DGTLSALSQKYFKRDIVSK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=252, Percent_Identity=45.6349206349206, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=45.8874458874459, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=32.9268292682927, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787088, Length=246, Percent_Identity=32.9268292682927, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=28.6259541984733, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27796; Mature: 27796

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKLSLCAAAALACAAVTAHAEQTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENSFDGLIPALQARKFDAINSAMNITSKRRQSIDFTPAIYVVPIVMIAKHG
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
SPLRPDVASLRGKHVGVLQGSSQEDFLKAHWATAGVAVVSYQDQDQIYADLVAGRLDAAV
CCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHH
QEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKNDKALQAKIVGALDALKK
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DGTLSALSQKYFKRDIVSK
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWKLSLCAAAALACAAVTAHAEQTTLRFGIEAAYPPFESKTPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENSFDGLIPALQARKFDAINSAMNITSKRRQSIDFTPAIYVVPIVMIAKHG
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
SPLRPDVASLRGKHVGVLQGSSQEDFLKAHWATAGVAVVSYQDQDQIYADLVAGRLDAAV
CCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHH
QEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKNDKALQAKIVGALDALKK
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DGTLSALSQKYFKRDIVSK
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]