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The map label for this gene is mhpA [H]

Identifier: 78065384

GI number: 78065384

Start: 822618

End: 824270

Strand: Direct

Name: mhpA [H]

Synonym: Bcep18194_A3910

Alternate gene names: 78065384

Gene position: 822618-824270 (Clockwise)

Preceding gene: 78065382

Following gene: 78065385

Centisome position: 22.27

GC content: 69.87

Gene sequence:

>1653_bases
ATGAGCATCGATTACCAGACGCTGAAGTTCGAATACCGTCCGCGCGCGCAGGACACCGCCGTTCACCCGGTGATCGTCGT
CGGCGCGGGCCCGGTGGGCCTTGCGGCGGCGATCGACCTGGCGCAGCAGGGCGTACCCGTCGTGCTGCTCGACGACGACG
ACACGCTGTCGACCGGCTCGCGCGCGATCTGCTTCGCGAAGCGCACGCTCGAGATCTTCGACCGGCTCGGCTGCGGCGAG
CGCTTCGTCGACAAGGGCGTGAGCTGGCACGTCGGCAAGGTGTTCCTGCAGGACGAGCAGCTGTACGCGTTCGACCTGCT
GCCCGAGGAAGGGCATGCGCGCCCCGCGTTCATCAACCTGCAGCAGTACTACGTCGAAGGCTACCTGGCCGACCGCGCGT
TCGAGCTGCCGAACATCGACATCCGCTGGAAGCACAAGGTGACGGGCATCGACCAGTCAGCCGAACACGCAGTGCTGACG
ATCGAGACGCCGGAAGGCGTCACGACGCTGCGCGCGCAGTACGTGATCGCGGCCGACGGCTCGCGCAGCCCGATGCGCAC
GATGATGGGCCTCGAAAGCCACGGCCGCACGTTCAAGGATCGCTTCCTGATCGCCGACGTGAAGATGAAGGCGGAGTTTC
CGACCGAGCGCTGGTTCTGGTTCGACCCGCCGTTCCACCCGAATCAGTCGGTGCTGCTGCATCGCCAGCCCGACAACGTG
TGGCGCATCGATTTCCAGCTCGGCTGGGATGCCGACCCCGTCGCCGAGAAGCAGCCGGAGCGCGTGATCCCGCGCGTGCG
CGCGCTGCTCGGGCCGGACGTCGAGTTCGAACTCGAATGGGTGAGCGTCTATACGTTCCGCTGCCAGCGGATGGAGTCGT
TCCGGCACGGCCGCGTGCTGTTCGCGGGCGACTCCGCGCACGGCGTGTCGCCGTTCGGCGCGCGTGGCGCGAACAGCGGC
GTGCAGGACGCCGACAACCTCGCATGGAAGCTGAAGCTGGTGCTCGACGGCGGTTCCGCCGACAGCCTGCTCGACACCTA
TGCGAGTGAGCGCGAGTTCGCGGCCGACGAGAACATCCGCAACTCGACGCGCTCGACCGACTTCATCACGCCGAAGAGCC
CGGTGTCGCGCGTGTTCCGCGACGCGACGCTGAAGCTCGCGCGCGACTGCGAGTTCGCGCGCAAGCTCGTGAACAGCGGC
CGCCTGTCGGTGCCGGCGGTGCTGGCCGATTCGCCGCTGAACACGCCGGACCGCAACGGCGACGCGTTCGCGGGCGCGAT
GCGGCCGGGCGCCGCGGCGGCCGATGCGCCGGTGCGTGCGCAGGGCGCGTCGGGCTGGCTGCTGCAACATTTGAGCAGCG
GCTTCACCGGCGTGCTGTTCGGGTTGCCGGGCGATGCGGCCGCGCTTGCGCAGGCGCTCGACGGCCTCGCGCTGCCGGTG
CGGCCCGTGCTCGTCGTGCCGGCCGGGCACGCGCAGCCGGCCGCGGGTGTCGTCGTCGTGGAGGACGTCGACGGCTTCGC
CGCGCAACGCTACGACGCGAAGCCCGGCACGTTCTACCTGCTGCGCCCCGACCAGCATGTCTGCGCGCGGACGCGCACGC
TCGACCGTCAGGCGCTGGCCGACGCACTGGCGCGCGCGACCTGCGCACGCTGA

Upstream 100 bases:

>100_bases
ACGTGGCGGTTGACAGGAATTGCACGCGGGAGAGCGGCGCACCGGCAGGTGACGGGCGGGATGACGGGCGCCCGGCAGGG
TTGAGGAGCGGATGGCGACC

Downstream 100 bases:

>100_bases
TAACGATAAAGACACCGGAGACTCCGTCATGCCCCCACTCGACACCCGCCCGCGTCTGGCTGACCCTGACGCGTTCTACG
AAGCGCTGATCGACATGCAT

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSIDYQTLKFEYRPRAQDTAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLGCGE
RFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGIDQSAEHAVLT
IETPEGVTTLRAQYVIAADGSRSPMRTMMGLESHGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHPNQSVLLHRQPDNV
WRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMESFRHGRVLFAGDSAHGVSPFGARGANSG
VQDADNLAWKLKLVLDGGSADSLLDTYASEREFAADENIRNSTRSTDFITPKSPVSRVFRDATLKLARDCEFARKLVNSG
RLSVPAVLADSPLNTPDRNGDAFAGAMRPGAAAADAPVRAQGASGWLLQHLSSGFTGVLFGLPGDAAALAQALDGLALPV
RPVLVVPAGHAQPAAGVVVVEDVDGFAAQRYDAKPGTFYLLRPDQHVCARTRTLDRQALADALARATCAR

Sequences:

>Translated_550_residues
MSIDYQTLKFEYRPRAQDTAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLGCGE
RFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGIDQSAEHAVLT
IETPEGVTTLRAQYVIAADGSRSPMRTMMGLESHGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHPNQSVLLHRQPDNV
WRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMESFRHGRVLFAGDSAHGVSPFGARGANSG
VQDADNLAWKLKLVLDGGSADSLLDTYASEREFAADENIRNSTRSTDFITPKSPVSRVFRDATLKLARDCEFARKLVNSG
RLSVPAVLADSPLNTPDRNGDAFAGAMRPGAAAADAPVRAQGASGWLLQHLSSGFTGVLFGLPGDAAALAQALDGLALPV
RPVLVVPAGHAQPAAGVVVVEDVDGFAAQRYDAKPGTFYLLRPDQHVCARTRTLDRQALADALARATCAR
>Mature_549_residues
SIDYQTLKFEYRPRAQDTAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLGCGER
FVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGIDQSAEHAVLTI
ETPEGVTTLRAQYVIAADGSRSPMRTMMGLESHGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHPNQSVLLHRQPDNVW
RIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMESFRHGRVLFAGDSAHGVSPFGARGANSGV
QDADNLAWKLKLVLDGGSADSLLDTYASEREFAADENIRNSTRSTDFITPKSPVSRVFRDATLKLARDCEFARKLVNSGR
LSVPAVLADSPLNTPDRNGDAFAGAMRPGAAAADAPVRAQGASGWLLQHLSSGFTGVLFGLPGDAAALAQALDGLALPVR
PVLVVPAGHAQPAAGVVVVEDVDGFAAQRYDAKPGTFYLLRPDQHVCARTRTLDRQALADALARATCAR

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=367, Percent_Identity=29.4277929155313, Blast_Score=132, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60404; Mature: 60273

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDYQTLKFEYRPRAQDTAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGS
CCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
RAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINL
EEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCEEEEH
QQYYVEGYLADRAFELPNIDIRWKHKVTGIDQSAEHAVLTIETPEGVTTLRAQYVIAADG
HHHHHHHHHHCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEEECC
SRSPMRTMMGLESHGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHPNQSVLLHRQPDNV
CCCHHHHHHHHHHCCCCCHHEEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCE
WRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMESFRHGRVL
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHCCCEEE
FAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGGSADSLLDTYASEREFAADENIR
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCC
NSTRSTDFITPKSPVSRVFRDATLKLARDCEFARKLVNSGRLSVPAVLADSPLNTPDRNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEECCCCCCCCCCC
DAFAGAMRPGAAAADAPVRAQGASGWLLQHLSSGFTGVLFGLPGDAAALAQALDGLALPV
CEEECCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCC
RPVLVVPAGHAQPAAGVVVVEDVDGFAAQRYDAKPGTFYLLRPDQHVCARTRTLDRQALA
CEEEEEECCCCCCCCCEEEEECCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
DALARATCAR
HHHHHHHCCC
>Mature Secondary Structure 
SIDYQTLKFEYRPRAQDTAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGS
CCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
RAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINL
EEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCEEEEH
QQYYVEGYLADRAFELPNIDIRWKHKVTGIDQSAEHAVLTIETPEGVTTLRAQYVIAADG
HHHHHHHHHHCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEEECC
SRSPMRTMMGLESHGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHPNQSVLLHRQPDNV
CCCHHHHHHHHHHCCCCCHHEEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCE
WRIDFQLGWDADPVAEKQPERVIPRVRALLGPDVEFELEWVSVYTFRCQRMESFRHGRVL
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHCCCEEE
FAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGGSADSLLDTYASEREFAADENIR
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCC
NSTRSTDFITPKSPVSRVFRDATLKLARDCEFARKLVNSGRLSVPAVLADSPLNTPDRNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEECCCCCCCCCCC
DAFAGAMRPGAAAADAPVRAQGASGWLLQHLSSGFTGVLFGLPGDAAALAQALDGLALPV
CEEECCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCC
RPVLVVPAGHAQPAAGVVVVEDVDGFAAQRYDAKPGTFYLLRPDQHVCARTRTLDRQALA
CEEEEEECCCCCCCCCEEEEECCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
DALARATCAR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA