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The map label for this gene is nitA [H]

Identifier: 78065266

GI number: 78065266

Start: 686007

End: 686930

Strand: Direct

Name: nitA [H]

Synonym: Bcep18194_A3790

Alternate gene names: 78065266

Gene position: 686007-686930 (Clockwise)

Preceding gene: 78065264

Following gene: 78065269

Centisome position: 18.57

GC content: 71.43

Gene sequence:

>924_bases
ATGTCCATGTCAGTCATCGCCGCGCTGCAGATCGGCGCATCGCCCGCCGGCACGCGCGCCACGCTCGACACGATCCTCGG
CTACGAAACCGCGATCCGCGACAGCGGCGCGTCGCTCGTCGTGCTGCCCGAGGCCGTGCTCGGCGGCTATCCGAAAGGCG
AGATCTTCGGCACGCGGCTCGGCTACCGGCTGCCCGAAGGCCGCGACGCGTATGCGCGCTACGCCGCGCAGGCGATCGAC
GTGCCGGGGCCGGAAACCGACGAGCTGGCCGCGCTGTCGCAGCGCACCGGTGCGAGCCTCGTGGTCGGCGTGATCGAGCG
CGGCGGCAGCACGCTGTACTGCACGGCGCTGTTCTTCGATCCGCGCGACGGGCTCGTCGCGAAGCACCGCAAGCTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGGCAGGGCGACGGCTCGACGCTGCCCGTCGTCGATACGGCGGCGGGCCGCGCG
GGCGCCGCGATCTGCTGGGAAAACCACATGCCGCTGCTGCGCTGCGCGATGTATGCGAAGGGCGTGCAGATCTGGTGCGC
GCCGACCGTCGACGAGCGCGACGTCTGGCAGAGCTCGATGCGGCATATCGCGCACGAAGGGCGCTGCTTCGTCGTCAGCG
CGTGCCAGGTGCAGCCGTCGCCGCGCGCGCTCGGCATCGACGTGCCGGGCTGGGATCCGGAGCGGCCGCTGATCCGCGGC
GGCAGCGTGATCGTCGGGCCGCTCGGCGACCTGCTGACGGAGCCGCTGATCGGCGAAGCCGGGCTCGTGACCGCGCGTAT
TGATACCGACGAACTCGTGCGGGCACGCTACGACTTCGACGTGGTCGGGCATTACGCGCGCCCCGACGTGTTCTCGCTGC
ACGTCGACGAGCGGGCGAAGCGGACGGTGGTGTTCGGCGAGTAG

Upstream 100 bases:

>100_bases
GCGATATCATCTGCATTCATGACACTTATCGTTCTATCGATTTGATCGATGACATCATATCGATAAAGATGGCGTTACCC
CATTCACCGGAGACGCCTCC

Downstream 100 bases:

>100_bases
GGAACGATGGGCCGGGCGCCGCACGGCGGCCCGGAACATGAACGGCGGCGGCGCGCAACGCGCACGCCGTCGTGCCGCGT
CAGCGCGTCAGCGCATCGGT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSMSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARPDVFSLHVDERAKRTVVFGE

Sequences:

>Translated_307_residues
MSMSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARPDVFSLHVDERAKRTVVFGE
>Mature_306_residues
SMSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAIDV
PGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAG
AAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRGG
SVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARPDVFSLHVDERAKRTVVFGE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=309, Percent_Identity=47.5728155339806, Blast_Score=287, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6322027, Length=199, Percent_Identity=65.3266331658292, Blast_Score=269, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322026, Length=112, Percent_Identity=45.5357142857143, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32941; Mature: 32810

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARPDVFSLHVDERAK
CCEEEECCHHHHCCCCCCCCCEEEEEECHHHHHHHHCCHHEECCCCCCCEEEEEECCCCC
RTVVFGE
EEEEECC
>Mature Secondary Structure 
SMSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARPDVFSLHVDERAK
CCEEEECCHHHHCCCCCCCCCEEEEEECHHHHHHHHCCHHEECCCCCCCEEEEEECCCCC
RTVVFGE
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]