Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 78065230

GI number: 78065230

Start: 646688

End: 647974

Strand: Direct

Name: gdhA [H]

Synonym: Bcep18194_A3754

Alternate gene names: 78065230

Gene position: 646688-647974 (Clockwise)

Preceding gene: 78065229

Following gene: 78065231

Centisome position: 17.51

GC content: 64.41

Gene sequence:

>1287_bases
ATGTCTTCGCAATTGCAGTCCATCCCGTCCTACCTTCACGCCGACGATCTCGGCCCGTGGGGCAACTACCTTCAGCAAGT
CGATCGCGTCGCGCCGTACCTCGGCTCGCTGTCGCGCTGGCTCGAAACGCTGAAGCGCCCGAAGCGCATCCTGATCGTCG
ACTGCCCCATCGAGCTCGATAACGGTACCGTCGCGCACTTCGAAGGCTATCGCGTGCAGCACAACGTGTCGCGCGGCCCG
GGCAAGGGCGGTGTGCGTTACCACCAGGACGTGACGCTGTCGGAAGTGATGGCACTGTCCGCATGGATGTCGGTCAAGAA
CGCGGCCGTGAACGTGCCGTACGGCGGCGCGAAGGGCGGTATCCGCGTCGACCCGCGCAAGCTGTCGCGTGGTGAGCTCG
AGCGCGTGACGCGCCGCTACACCAGCGAAATCGGCATCATCATCGGCCCGAACACCGACATTCCGGCGCCGGACGTCAAC
ACCAACGAACAGGTGATGGCGTGGATGATGGACACGTACTCGATGAACCAGGGCCAGACGTCGACCGGCGTCGTGACCGG
CAAGCCGATCGCACTCGGCGGTTCGCTCGGCCGCAAGGAAGCAACGGGCCGCGGCGTGTTCGTCGTCGGCAGCGAAGCGG
CGAAGAAGAAGGGCCTCGAGATCGAAGGCGCACGAATCGCGGTGCAGGGCTTCGGCAACGTCGGCGGCATCGCGGCGAAG
CTGTTCCAGGAAGCGGGTGCGAAGGTGATCGCGGTGCAGGATCACACGGGCACGATCTACCAGCCGGCCGGCCTCGATTC
GAACAAGCTGCTCGACCACGTCGCACGCACGGGCGGTGTCGCGGGCTTCGAAGGCGCGGAACCGATGCCGAATGACGAGT
TCTGGACCGTCGAAACCGACATCCTGATCCCGGCGGCACTGGAAAACCAGATCACCGAGAAGAACGCAGGGAAGATCCGC
ACGAAGATCATCGTCGAAGGCGCGAACGGCCCGACGACGACGGCGGCGGACGACATCCTGACCGCGAACGGCGTGCTGGT
GATCCCGGACGTGATCGCGAACGCAGGTGGCGTGACCGTGTCGTATTTCGAATGGGTGCAGGATTTCTCGAGCTTCTTCT
GGACGGAAGACGAGATCAACCACCGTCTCGAACGCGTGATGCGCGAAGCGTTCTCCGGCGTGTGGGCGGTCGCGGAAGAG
CACAAGGTGTCGGTGCGTACGGCAGCGTTCATCGTTGCGTGCAAGCGCATCCTGATGGCGCGCGAAATGCGCGGCCTGTA
CCCCTGA

Upstream 100 bases:

>100_bases
GCGATCATGCAAGAAACGCATAATCGAATAAGCAAACGGCATTGGATAAAAAAAACGGGTTTTTCGACAATGTGCGCACC
TGTTGACCGTTGGAGCATCC

Downstream 100 bases:

>100_bases
TTCGCGGTTGCCGATTTCAGATCGGTTGACTACGATCAATCCATGACGCAATCCATCCGGTTGCGTGCATGAACCCTCGC
GGGCGGTGCCGAATCCGGTA

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDCPIELDNGTVAHFEGYRVQHNVSRGP
GKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVN
TNEQVMAWMMDTYSMNQGQTSTGVVTGKPIALGGSLGRKEATGRGVFVVGSEAAKKKGLEIEGARIAVQGFGNVGGIAAK
LFQEAGAKVIAVQDHTGTIYQPAGLDSNKLLDHVARTGGVAGFEGAEPMPNDEFWTVETDILIPAALENQITEKNAGKIR
TKIIVEGANGPTTTAADDILTANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFSGVWAVAEE
HKVSVRTAAFIVACKRILMAREMRGLYP

Sequences:

>Translated_428_residues
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDCPIELDNGTVAHFEGYRVQHNVSRGP
GKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVN
TNEQVMAWMMDTYSMNQGQTSTGVVTGKPIALGGSLGRKEATGRGVFVVGSEAAKKKGLEIEGARIAVQGFGNVGGIAAK
LFQEAGAKVIAVQDHTGTIYQPAGLDSNKLLDHVARTGGVAGFEGAEPMPNDEFWTVETDILIPAALENQITEKNAGKIR
TKIIVEGANGPTTTAADDILTANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFSGVWAVAEE
HKVSVRTAAFIVACKRILMAREMRGLYP
>Mature_427_residues
SSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDCPIELDNGTVAHFEGYRVQHNVSRGPG
KGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNT
NEQVMAWMMDTYSMNQGQTSTGVVTGKPIALGGSLGRKEATGRGVFVVGSEAAKKKGLEIEGARIAVQGFGNVGGIAAKL
FQEAGAKVIAVQDHTGTIYQPAGLDSNKLLDHVARTGGVAGFEGAEPMPNDEFWTVETDILIPAALENQITEKNAGKIRT
KIIVEGANGPTTTAADDILTANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINHRLERVMREAFSGVWAVAEEH
KVSVRTAAFIVACKRILMAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=370, Percent_Identity=43.2432432432432, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI4885281, Length=370, Percent_Identity=41.6216216216216, Blast_Score=279, Evalue=4e-75,
Organism=Escherichia coli, GI1788059, Length=366, Percent_Identity=33.879781420765, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=392, Percent_Identity=40.5612244897959, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319256, Length=402, Percent_Identity=29.6019900497512, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=387, Percent_Identity=30.2325581395349, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=42.5414364640884, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45549226, Length=354, Percent_Identity=43.2203389830509, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24649283, Length=358, Percent_Identity=43.5754189944134, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649460, Length=301, Percent_Identity=43.5215946843854, Blast_Score=234, Evalue=6e-62,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46378; Mature: 46247

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDCPIELD
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECC
NGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGG
CCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCC
IRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTYSMNQGQT
EEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
STGVVTGKPIALGGSLGRKEATGRGVFVVGSEAAKKKGLEIEGARIAVQGFGNVGGIAAK
CCCEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCEECCCEEEEEECCCCHHHHHH
LFQEAGAKVIAVQDHTGTIYQPAGLDSNKLLDHVARTGGVAGFEGAEPMPNDEFWTVETD
HHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
ILIPAALENQITEKNAGKIRTKIIVEGANGPTTTAADDILTANGVLVIPDVIANAGGVTV
EEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHEECCCEEEECHHHCCCCCCCH
SYFEWVQDFSSFFWTEDEINHRLERVMREAFSGVWAVAEEHKVSVRTAAFIVACKRILMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHHHHHH
REMRGLYP
HHHHCCCC
>Mature Secondary Structure 
SSQLQSIPSYLHADDLGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILIVDCPIELD
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECC
NGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGG
CCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCC
IRVDPRKLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTYSMNQGQT
EEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
STGVVTGKPIALGGSLGRKEATGRGVFVVGSEAAKKKGLEIEGARIAVQGFGNVGGIAAK
CCCEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCEECCCEEEEEECCCCHHHHHH
LFQEAGAKVIAVQDHTGTIYQPAGLDSNKLLDHVARTGGVAGFEGAEPMPNDEFWTVETD
HHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
ILIPAALENQITEKNAGKIRTKIIVEGANGPTTTAADDILTANGVLVIPDVIANAGGVTV
EEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHEECCCEEEECHHHCCCCCCCH
SYFEWVQDFSSFFWTEDEINHRLERVMREAFSGVWAVAEEHKVSVRTAAFIVACKRILMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHHHHHH
REMRGLYP
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]