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The map label for this gene is pnp [H]

Identifier: 78048389

GI number: 78048389

Start: 3222209

End: 3224323

Strand: Reverse

Name: pnp [H]

Synonym: XCV2833

Alternate gene names: 78048389

Gene position: 3224323-3222209 (Counterclockwise)

Preceding gene: 78048390

Following gene: 78048385

Centisome position: 62.26

GC content: 62.93

Gene sequence:

>2115_bases
GTGGCAAAAATCACCAAAACCTTCCAGTACGGCAAGCACACCGTCACTCTTGAGACGGGCGAAATCGCTCGCCAGGCAGG
CGGCGCCGTGATCGTCAAGTTCGACGACACCGTACTGCTGGTCACCGCCGTCGCCGCCAAGAGCGCGCGCGAAGGGCAGG
ACTTCTTTCCTCTGACGGTCGATTATCAGGAGAAGTTCTACGCTGGCGGCCGCATCCCGGGCGGCTTCTTCAAGCGCGAA
GGACGCGCGACCGAGAAGGAAACGTTGATCTCGCGCCTGATCGATCGTCCGATCCGTCCGCTGTTCCCGGAGGACTACAA
GAACGAAGTGCAGATCATCGCCACCGTGATGTCGATGAACCCGGACATCGACGGCGACATCGCCGCGCTGATCGGCGCCT
CGGCTGCCCTGTCGCTGGCCGGCACCCCCTTCAAGGGCCCGATCGGCGCTGCCAAGGTCGGCTACAAGAACGGTGAATAC
ATCCTCAACCCGACCGTGACCGAGCTGAAGGATTCGCAGCTGGAGCTGGTCGTGGCCGGTACCGCCAACGCGGTGCTGAT
GGTCGAGTCCGAAGCCGAGCTGCTGTCCGAAGAAGTGATGCTGGGCGCAGTGACGTTCGGTCATCGCGAGATGCAGAAGG
TCATCAACGTCATCAACGAGCTGACCGTCGAAGCCGGCACCAAGCCGTCCGACTGGGTGGCTCCGGCAAAGAACGAGGGC
ATGATCGCCGCACTGAAGGAAGCTGTGGGCGATCAGCTCGCGTCGGCATTCCAGGTGCGCGACAAGCTGCAGCGCCGCGA
TGCGATTTCGGCGATCAAGAAGGACGTGCTGGGCGCACTGGCGCCGCGCGCCACCATCGAAGGCTGGGCTGCCGGCGATC
TGGCCAAGGAATTCGGCGAGCTGGAATACCAGACCATGCGTGGTTCGGTGTTGAGCAGCAAGGTGCGTATCGACGGCCGT
GCACTGGACACCGTGCGTCCGATCAGCGCCAAGGCTGGCGTGTTGCCGCGGACCCATGGCTCGGCGCTGTTTACCCGCGG
CGAGACGCAGGCGATCGTGATCACCACGCTGGGCACCGCGCGCGACGGTCAGGTCATCGACGCAGTGTCGGGCGAGTACA
AGGAAAACTTCCTGTTCCATTACAACTTCCCTCCGTACTCGGTGGGCGAGTGCGGCCGTTTCGGTGCGCCGAAGCGCCGC
GAAATCGGCCACGGCCGCCTGGCCAAGCGCGGCGTGCTGGCTGTGATGCCGACCCTGGAAGAATTCCCGTACACCATTCG
CGTGGTCTCGGAAATCACCGAGTCCAACGGTTCGTCCTCGATGGCATCGGTCTGCGGCAGCTCGCTGGCGCTGATGGATG
CCGGCGTGCCGATCAAGGCGCCGGTCGCGGGTATCGCAATGGGTCTGGTCAAGGAAGGCAACGACTTCGTCGTGCTCTCC
GACATCCTGGGTGACGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGTACTGCCGAAGGCGTGTCCGCGCTGCA
GATGGACATCAAGATCGAAGGCATCACCGAAGAGATCATGAAGCAGGCCTTGCAGCAGGCCAAGGCTGGCCGCCTGCACA
TTCTGGGCGAGATGGCCCATGCGCTGACCACCCCGCGTCAGGAATTGAGCGACTACGCGCCGCGCCTGCTGACCATCAAG
ATCCACCCGGACAAGATCCGCGAAGTGATCGGCAAGGGTGGCTCGACCATCCAGGCCATCACCAAGGAAACCGGTACGCA
GATCGACATCCAGGACGATGGCACGATCATCATCGCCTCCGTCAATGCCATTGCCGCGCAGGCTGCGAAGTCGCGCATCG
AGCAGATCACCTCGGACGTGGAGCCGGGCCGCATCTACGAAGGCAAAGTCGCCAAGATCATGGACTTCGGTGCGTTCGTC
ACCATCCTGCCGGGCAAGGACGGCCTGGTGCACGTGTCGCAGATTTCCAGCGAGCGTGTGGAGAAGGTCGGCGACAAGTT
GAAGGAAGGCGATCTGGTCCGCGTCAAGGTGCTGGAAGTGGACAAGCAGGGCCGCATCCGTCTGTCGATCAAGGCAGTGG
AAGAAGGCGAAGGCGTGCAGGCGTCGGCCGAGTAA

Upstream 100 bases:

>100_bases
GCAATACGCACTCTTGGGCCGGAGCCTCCCGGCCGCCCGTTCTCGGCATGGGTCGACGACGGTCCAACGCAGACAGCAAT
ACCTAAGGGAAAAAACCTCC

Downstream 100 bases:

>100_bases
TCGCAGCGTGCTGATGCAGCATGCGTGATGCATGACGAAAGAGCGGGCTTCGGCCCGCTTTTTTGTTGTGCACTTGATCC
CTTCTCGCTGCGGGAGAAGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKFDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEY
ILNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNEG
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSSKVRIDGR
ALDTVRPISAKAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE

Sequences:

>Translated_704_residues
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKFDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEY
ILNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNEG
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSSKVRIDGR
ALDTVRPISAKAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
>Mature_703_residues
AKITKTFQYGKHTVTLETGEIARQAGGAVIVKFDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKREG
RATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYI
LNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNEGM
IAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSSKVRIDGRA
LDTVRPISAKAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRRE
IGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLSD
ILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIKI
HPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVT
ILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=36.376404494382, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=64.4604316546763, Blast_Score=892, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=82, Percent_Identity=46.3414634146341, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=706, Percent_Identity=33.0028328611898, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=81, Percent_Identity=46.9135802469136, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=653, Percent_Identity=36.6003062787136, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75511; Mature: 75379

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKFDDTVLLVTAVAAKSAREGQDFFPLTV
CCCCCCHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHHHHHCCCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYILNPTVTELKDSQLELVVAG
CCCCCCHHHHHHCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNEG
CCCEEEEEECHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LEYQTMRGSVLSSKVRIDGRALDTVRPISAKAGVLPRTHGSALFTRGETQAIVITTLGTA
CHHHHHHCHHHHCEEEECCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPTLE
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
HCCCCEEEHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAH
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKITKTFQYGKHTVTLETGEIARQAGGAVIVKFDDTVLLVTAVAAKSAREGQDFFPLTV
CCCCCHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHHHHHCCCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYILNPTVTELKDSQLELVVAG
CCCCCCHHHHHHCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNEG
CCCEEEEEECHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LEYQTMRGSVLSSKVRIDGRALDTVRPISAKAGVLPRTHGSALFTRGETQAIVITTLGTA
CHHHHHHCHHHHCEEEECCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPTLE
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
HCCCCEEEHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAH
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]