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The map label for this gene is egl4 [H]

Identifier: 78048260

GI number: 78048260

Start: 3063572

End: 3065332

Strand: Reverse

Name: egl4 [H]

Synonym: XCV2704

Alternate gene names: 78048260

Gene position: 3065332-3063572 (Counterclockwise)

Preceding gene: 78048261

Following gene: 78048258

Centisome position: 59.19

GC content: 65.25

Gene sequence:

>1761_bases
ATGACTATCTTCAAAACTCTGCTCACCAGCCTGATGATCGTTTCGCCCGTGCTCGCCTGCGCTGCAGAGACAACCGGCAG
GCAGGAGGCCGTGGCGGCCAGCGACGCCATCCACGTCAACCAGCTGGGCTATCTGCCGGGCTCGGCAAAAATGGCGATCG
TCGGGCTGCCGCCGCAGGGCACCGCGCAGCGTTCGGACCGCTTCACCGTCGAAGATGCACAGGGACGCAGCGTGTTGCAG
GGAACCCTGCAGCCGGCCGCGATGTGGTCGCCGGCCGGACAGCAGGCGCGGGTGGCGGATTTTTCCGGGCTGCGTGCGGT
CGGAACCTATCGGTTGAAGGTCGACGGGCTGCCGGCATCGGAGACCTTCGCCATCAACGCGCATGCCTATCAGCCCCTGG
TCGATGCAGCGTTGAAGGCGTTCTACTTCAACCGTGCCAGCACCGCGCTGCCGGCACGCTACGCCGGGCGCTATGCGCGC
GCGGCCGGGCACCCGGATACGCAGGTGCGCATCCATCCGTCCGCAGCGTCTGCAACGCGTCCGACCGATAGCGTGATCAG
CGCGCCCAAGGGCTGGTACGACGCGGGCGACTACAACAAGTACATCGTCAATTCCGGCATCAGCACCTACACCTTGCTGG
CGGCCTACGAGCAGTATCCGGCGTTCTTCAGGTCCAAGCCGCTGACCATCCCCGACGATGCGCCCGGCGTGCCCGGCATC
CTGCAGGAGGCCTGGTGGAATCTGGACTGGATGCTCGCCATGCAGGACCCGGCCGATGGCGGCGTGTACCACAAGCTCAC
CGACAAGCAGTTCGATGGCTTGGTGATGCCGGATCAGGCCACGCAGCAACGCTACGTGGTGATGAAGACCAATGCCGCGA
CCTTGGACTTCGCTGCGGTGATGGCCGTCGCCAGTCGCGTATATGCGCCATTTGAAAAGCAGTATCCGGGGCTGTCGGCG
CGCATGTTGAAAGCCTCGCGGGCTGCGTGGGCGTGGGCGAAGCAGCATCCGGACGTGATCTACCAACAGCCCAGCGACGT
ACGCACCGGCGGTTACGACGATGCACACCTGGATGACGAATTCGCCTGGGCGGCGGCCGAGTTGTACATCGCCACCGGCG
AGGATGGGTTCTACGACGCGATGATCGCGCGCAACGTGCCGGCCACCGTGCCGTCGTGGGGCAATGTCGGTGGGCTGGCC
TGGATGTCGTTGGCCGCGCATCGCGACCGACTCACGCCGCATGCCGATCGCGCGCGGATCGCACGGGAGATCACCGGACT
GGCACAACAGCTGGCCGACACCTGGCAATCATCGGCATGGCGGCTGGCGATGAACGACAGCGATTTCGTGTGGGGCAGCA
ATGCAGGGGTGCTCAACCGCGCGATGATGCTGCTGCAGGGCTACCGGTTGACGCAGCAGCGGCAATTTCTGGATGCGGCG
CAATCGCAGCTGGACTACATCCTGGGCCGCAATCCGCTGGGACTGTCGTTCGTGACCGGGATAGGCAAACGCACCCCGAT
GCATATCCATCACCGCCCCTCCGAGGCCGATGGGATCGAAGCGCCGGTGCCGGGTTGGCTGGTCGGCGGCCCGCAGCCGG
GGCAGCAGGATGCCAAGGAGTGCAAGGTGCCGTACCCCTCCAAACGGCCGGCGTTGTCGTACCTGGACAACTTCTGCAGC
TACTCCACCAACGAGGTGGCGATCAACTGGAATGCGCCGCTGGTGTATGTGAGCGCGGCGATCGAGGCGTCGACGCGCTA
A

Upstream 100 bases:

>100_bases
ATCCGCGCAAGGGGCGCATTGCTGCAGCGATGCGGATTGACGCATCGGCTATGCCGGCATTGGACTTGTCGCTGCAAGAT
AATGCGGCGACCATCGTGGC

Downstream 100 bases:

>100_bases
AGCGTCGCTATCAAGCCGATGCCGCGGCTGTTCCTTTTCCCGTCGGGAGAAGGTGGCGCGCAGCGCCGGATGAGGGTACG
GCGTCGGCAGGAATACTGAT

Product: cellulase precursor

Products: NA

Alternate protein names: EGD; Cellulase D; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTIFKTLLTSLMIVSPVLACAAETTGRQEAVAASDAIHVNQLGYLPGSAKMAIVGLPPQGTAQRSDRFTVEDAQGRSVLQ
GTLQPAAMWSPAGQQARVADFSGLRAVGTYRLKVDGLPASETFAINAHAYQPLVDAALKAFYFNRASTALPARYAGRYAR
AAGHPDTQVRIHPSAASATRPTDSVISAPKGWYDAGDYNKYIVNSGISTYTLLAAYEQYPAFFRSKPLTIPDDAPGVPGI
LQEAWWNLDWMLAMQDPADGGVYHKLTDKQFDGLVMPDQATQQRYVVMKTNAATLDFAAVMAVASRVYAPFEKQYPGLSA
RMLKASRAAWAWAKQHPDVIYQQPSDVRTGGYDDAHLDDEFAWAAAELYIATGEDGFYDAMIARNVPATVPSWGNVGGLA
WMSLAAHRDRLTPHADRARIAREITGLAQQLADTWQSSAWRLAMNDSDFVWGSNAGVLNRAMMLLQGYRLTQQRQFLDAA
QSQLDYILGRNPLGLSFVTGIGKRTPMHIHHRPSEADGIEAPVPGWLVGGPQPGQQDAKECKVPYPSKRPALSYLDNFCS
YSTNEVAINWNAPLVYVSAAIEASTR

Sequences:

>Translated_586_residues
MTIFKTLLTSLMIVSPVLACAAETTGRQEAVAASDAIHVNQLGYLPGSAKMAIVGLPPQGTAQRSDRFTVEDAQGRSVLQ
GTLQPAAMWSPAGQQARVADFSGLRAVGTYRLKVDGLPASETFAINAHAYQPLVDAALKAFYFNRASTALPARYAGRYAR
AAGHPDTQVRIHPSAASATRPTDSVISAPKGWYDAGDYNKYIVNSGISTYTLLAAYEQYPAFFRSKPLTIPDDAPGVPGI
LQEAWWNLDWMLAMQDPADGGVYHKLTDKQFDGLVMPDQATQQRYVVMKTNAATLDFAAVMAVASRVYAPFEKQYPGLSA
RMLKASRAAWAWAKQHPDVIYQQPSDVRTGGYDDAHLDDEFAWAAAELYIATGEDGFYDAMIARNVPATVPSWGNVGGLA
WMSLAAHRDRLTPHADRARIAREITGLAQQLADTWQSSAWRLAMNDSDFVWGSNAGVLNRAMMLLQGYRLTQQRQFLDAA
QSQLDYILGRNPLGLSFVTGIGKRTPMHIHHRPSEADGIEAPVPGWLVGGPQPGQQDAKECKVPYPSKRPALSYLDNFCS
YSTNEVAINWNAPLVYVSAAIEASTR
>Mature_585_residues
TIFKTLLTSLMIVSPVLACAAETTGRQEAVAASDAIHVNQLGYLPGSAKMAIVGLPPQGTAQRSDRFTVEDAQGRSVLQG
TLQPAAMWSPAGQQARVADFSGLRAVGTYRLKVDGLPASETFAINAHAYQPLVDAALKAFYFNRASTALPARYAGRYARA
AGHPDTQVRIHPSAASATRPTDSVISAPKGWYDAGDYNKYIVNSGISTYTLLAAYEQYPAFFRSKPLTIPDDAPGVPGIL
QEAWWNLDWMLAMQDPADGGVYHKLTDKQFDGLVMPDQATQQRYVVMKTNAATLDFAAVMAVASRVYAPFEKQYPGLSAR
MLKASRAAWAWAKQHPDVIYQQPSDVRTGGYDDAHLDDEFAWAAAELYIATGEDGFYDAMIARNVPATVPSWGNVGGLAW
MSLAAHRDRLTPHADRARIAREITGLAQQLADTWQSSAWRLAMNDSDFVWGSNAGVLNRAMMLLQGYRLTQQRQFLDAAQ
SQLDYILGRNPLGLSFVTGIGKRTPMHIHHRPSEADGIEAPVPGWLVGGPQPGQQDAKECKVPYPSKRPALSYLDNFCSY
STNEVAINWNAPLVYVSAAIEASTR

Specific function: This enzyme catalyzes the endohydrolysis of 1,4-beta- glucosidic linkages in cellulose, lichenin and cereal beta-D- glucans [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 9 (cellulase E) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR016134
- InterPro:   IPR002105
- InterPro:   IPR018242
- InterPro:   IPR001701
- InterPro:   IPR018221
- InterPro:   IPR004197
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF02927 CelD_N; PF00404 Dockerin_1; PF00759 Glyco_hydro_9 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 63880; Mature: 63748

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00592 GLYCOSYL_HYDROL_F9_1 ; PS00698 GLYCOSYL_HYDROL_F9_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFKTLLTSLMIVSPVLACAAETTGRQEAVAASDAIHVNQLGYLPGSAKMAIVGLPPQG
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHCCCCCCCCCEEEEECCCCC
TAQRSDRFTVEDAQGRSVLQGTLQPAAMWSPAGQQARVADFSGLRAVGTYRLKVDGLPAS
CCCCCCCEEEECCCCCHHHHHCCCCHHHCCCCCCCCEECCCCCCEEEEEEEEEECCCCCC
ETFAINAHAYQPLVDAALKAFYFNRASTALPARYAGRYARAAGHPDTQVRIHPSAASATR
CEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PTDSVISAPKGWYDAGDYNKYIVNSGISTYTLLAAYEQYPAFFRSKPLTIPDDAPGVPGI
CCHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LQEAWWNLDWMLAMQDPADGGVYHKLTDKQFDGLVMPDQATQQRYVVMKTNAATLDFAAV
HHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCEECCCCCCCCEEEEEECCCCHHHHHHH
MAVASRVYAPFEKQYPGLSARMLKASRAAWAWAKQHPDVIYQQPSDVRTGGYDDAHLDDE
HHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCH
FAWAAAELYIATGEDGFYDAMIARNVPATVPSWGNVGGLAWMSLAAHRDRLTPHADRARI
HHEEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
AREITGLAQQLADTWQSSAWRLAMNDSDFVWGSNAGVLNRAMMLLQGYRLTQQRQFLDAA
HHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
QSQLDYILGRNPLGLSFVTGIGKRTPMHIHHRPSEADGIEAPVPGWLVGGPQPGQQDAKE
HHHHHHHHCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEECCCCCCCHHHHHH
CKVPYPSKRPALSYLDNFCSYSTNEVAINWNAPLVYVSAAIEASTR
CCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TIFKTLLTSLMIVSPVLACAAETTGRQEAVAASDAIHVNQLGYLPGSAKMAIVGLPPQG
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHCCCCCCCCCEEEEECCCCC
TAQRSDRFTVEDAQGRSVLQGTLQPAAMWSPAGQQARVADFSGLRAVGTYRLKVDGLPAS
CCCCCCCEEEECCCCCHHHHHCCCCHHHCCCCCCCCEECCCCCCEEEEEEEEEECCCCCC
ETFAINAHAYQPLVDAALKAFYFNRASTALPARYAGRYARAAGHPDTQVRIHPSAASATR
CEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PTDSVISAPKGWYDAGDYNKYIVNSGISTYTLLAAYEQYPAFFRSKPLTIPDDAPGVPGI
CCHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LQEAWWNLDWMLAMQDPADGGVYHKLTDKQFDGLVMPDQATQQRYVVMKTNAATLDFAAV
HHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCEECCCCCCCCEEEEEECCCCHHHHHHH
MAVASRVYAPFEKQYPGLSARMLKASRAAWAWAKQHPDVIYQQPSDVRTGGYDDAHLDDE
HHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCH
FAWAAAELYIATGEDGFYDAMIARNVPATVPSWGNVGGLAWMSLAAHRDRLTPHADRARI
HHEEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
AREITGLAQQLADTWQSSAWRLAMNDSDFVWGSNAGVLNRAMMLLQGYRLTQQRQFLDAA
HHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
QSQLDYILGRNPLGLSFVTGIGKRTPMHIHHRPSEADGIEAPVPGWLVGGPQPGQQDAKE
HHHHHHHHCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEECCCCCCCHHHHHH
CKVPYPSKRPALSYLDNFCSYSTNEVAINWNAPLVYVSAAIEASTR
CCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3024110 [H]