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The map label for this gene is pldA [C]

Identifier: 78047076

GI number: 78047076

Start: 1716108

End: 1717310

Strand: Reverse

Name: pldA [C]

Synonym: XCV1520

Alternate gene names: 78047076

Gene position: 1717310-1716108 (Counterclockwise)

Preceding gene: 78047077

Following gene: 78047072

Centisome position: 33.16

GC content: 62.93

Gene sequence:

>1203_bases
ATGCAGACGCGACCGTTAAGCTGCGCATCTCTGCGCCTGCCTGGCGCCTTTCCCTCGGCCAGACTGCCCATGTCCAAGCA
CCACACCCGCCCCCTGATGCTGATCGCACTGGCCGCCGCGCCATTGGCCCATGCGCAGGAAATCGCACCGCAGCCGGCCT
CGCCGCAAGCCTGCACCTCGGTGACGTCCGATGCCGCTCGCCTGGCCTGCTACGACAAGGCACTGGGCTACACGCCGCAG
CAAACTGCGGAGGCAGATGCCGCCGCGCAATTGGCCAAGCAAAAGGAGACCAAGCCCGACGATACGCGCACCATCAGCCG
GGTCGGCGATTTCTTCCGCGCCGACGGCCAGGACCAGCCGAAAGAAGAAGCCGTGGCCAATGCCGGCCGTGGCTCCTTGC
TGGACCGACGCTGGGAACTGGCCAAGGACTCCAAGCTGGGCACCTTCCAGCTGCGCGGTTACAAGCCGGTGTACCTGTTG
CCGGCATTCTGGACCAGCGACACCAACCGCACCCCGCAGTCGCCCAATCCGGCCAACTCGGTCAACACGCCGCAGCAGCT
GGATAGCACCGAGCTCAAGTTCCAGCTCAGCTTCAAGACCAAGGTGGTGGAAGACCTGTTCGGCGACAACGGCGATATCT
GGATGGGCTACACCCAGAGCTCGCGCTGGCAGGCCTATAACGCCGATGCGTCGCGCCCGTTCCGCGAGACCAATTACGAG
CCCGAAGCGATGCTGGTCTTTCGCAACAACTACAGCATCTTCGGCTGGAAGGGCCGCATGAGCGGCATCAGCCTCAATCA
CATGTCCAACGGGCGCGCCGACCCGCTGTCGCGCTCGTGGAATCGGGTGATCCTGAACTTCGGACTGGATCGGGAGAACT
GGGCGCTGACACTGCGCCCGTGGTTCCGTATCAAGGAAGACCGCGCCGACGACAACAATCCTGATATCGAGGATTACATG
GGCCGCGGCGATGCGACGCTGGTGTACAACAAGGATGGTCACGAATTCGCGCTGATCGCACGACATTCGCTGCGTGGCGG
CGACCGCTCGCACGGTTCGGTGCAGCTGGACTACGGCTTCCCCATCACCAACCTGTTGCGCGGCCATGTGCAGGTATTCG
ATGGCTACGGCGAAAGCATGATCGACTACAACCACAAGGCAACCTACATCGGTGTGGGCGTGTCGTTGCTGGAGTGGTTC
TAA

Upstream 100 bases:

>100_bases
CCACATTCCATGCGCATACCCGCTCCACGGCAACACGTCGCAGGCACAGACTGAGCGACCGAATTCGATCGAATGTTATG
TCGCCGTGTACACACGGCGT

Downstream 100 bases:

>100_bases
GCGCAGCTCACAAAACCACTGCGCTCACCGCCGGCCGCATGCGGCCGGTGCGCGGAATGGCATGTACGACTCGTACACTG
CCATTCCTGTGCGCCATCCT

Product: outer membrane phospholipase A

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQTRPLSCASLRLPGAFPSARLPMSKHHTRPLMLIALAAAPLAHAQEIAPQPASPQACTSVTSDAARLACYDKALGYTPQ
QTAEADAAAQLAKQKETKPDDTRTISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLL
PAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNADASRPFRETNYE
PEAMLVFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPDIEDYM
GRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF

Sequences:

>Translated_400_residues
MQTRPLSCASLRLPGAFPSARLPMSKHHTRPLMLIALAAAPLAHAQEIAPQPASPQACTSVTSDAARLACYDKALGYTPQ
QTAEADAAAQLAKQKETKPDDTRTISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLL
PAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNADASRPFRETNYE
PEAMLVFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPDIEDYM
GRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF
>Mature_400_residues
MQTRPLSCASLRLPGAFPSARLPMSKHHTRPLMLIALAAAPLAHAQEIAPQPASPQACTSVTSDAARLACYDKALGYTPQ
QTAEADAAAQLAKQKETKPDDTRTISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLL
PAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNADASRPFRETNYE
PEAMLVFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPDIEDYM
GRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=280, Percent_Identity=35.7142857142857, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 44857; Mature: 44857

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRPLSCASLRLPGAFPSARLPMSKHHTRPLMLIALAAAPLAHAQEIAPQPASPQACTS
CCCCCCCCEEEECCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHH
VTSDAARLACYDKALGYTPQQTAEADAAAQLAKQKETKPDDTRTISRVGDFFRADGQDQP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
KEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLLPAFWTSDTNRTPQSPNPANS
HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
VNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNADASRPFRETNYE
CCCCHHCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHCCCC
PEAMLVFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRP
CCEEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCEEEEEECCCCCCCEEEEEC
WFRIKEDRADDNNPDIEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGF
CEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEECC
PITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF
CHHHHHHHHHEEECCCCCHHHCCCCCEEEEECCHHHHHCC
>Mature Secondary Structure
MQTRPLSCASLRLPGAFPSARLPMSKHHTRPLMLIALAAAPLAHAQEIAPQPASPQACTS
CCCCCCCCEEEECCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHH
VTSDAARLACYDKALGYTPQQTAEADAAAQLAKQKETKPDDTRTISRVGDFFRADGQDQP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
KEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLLPAFWTSDTNRTPQSPNPANS
HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
VNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNADASRPFRETNYE
CCCCHHCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHCCCC
PEAMLVFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRP
CCEEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCEEEEEECCCCCCCEEEEEC
WFRIKEDRADDNNPDIEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGF
CEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEECC
PITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF
CHHHHHHHHHEEECCCCCHHHCCCCCEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]