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The map label for this gene is hisF

Identifier: 78044843

GI number: 78044843

Start: 965723

End: 966502

Strand: Direct

Name: hisF

Synonym: CHY_1090

Alternate gene names: 78044843

Gene position: 965723-966502 (Clockwise)

Preceding gene: 78043937

Following gene: 78044810

Centisome position: 40.21

GC content: 48.85

Gene sequence:

>780_bases
ATGTTAACGGTGCGGATAATTCCCTGCCTGGATGTGAATAAAGGGCGGGTGGTAAAAGGGGTAAATTTCTTAAACCTTGT
GGATGCCGGAGACCCGGTTGAGCTTGCCGCTTTTTACGACCGGGAAGGTGCCGATGAGGTGGTATTTTTGGATATTACTG
CTTCCTTTGAGGGGCGACAAACCATGGTGGAAGTGGCCAAGCGGACGGCTACAACCCTTTCCATCCCCTTTACCATTGGC
GGAGGTATAAGAAATATTGATGATATCCGAGCCCTTTTGGCTTCCGGGGCGGATAAAGTATCGATTAATTCGGCGGCGGT
AAAAAATCCCGAACTTGTCCGGGAAGCGGCTTTAAAGTTTGGCAGCCAGTGTATTGTGGTGGCTATCGATGCCAAAAGAA
AAAAAGATGGTGGCTTTGAAGTTTATATCCACGGTGGGCGTACTCCCACCGGAATAGATGCGGTAGAATGGGCTAAGGAA
GTGGAGAGACTCGGGGCCGGAGAAATCTTACTTACCAGTATGGATAGAGATGGAACGAAAGATGGCTATGACCTCGAACT
TACCCGGGAAATTGCCGATGCGGTGAAAATTCCGGTAATTGCCTCGGGGGGTGTGGGAGAGCTTTCCCATTTTTACGAAG
GAGTGGTTTTGGGGCACGCTTCGGCTCTTTTGGCAGCTTCGGTGTTTCACTTTGGTAAATTTACCATTAAAGAAGTAAAG
AGGTACTTAAAAGAGCGGGGGATACCGGTCCGATTGGGGGATACCGAAAATGGAACTTAA

Upstream 100 bases:

>100_bases
TCGGGGAAAACAAAATCTACGGGGTTATTGCCGGAAAAGCAATATTAGAAGGTAAAATTGATTTAAAAGAAGGGATTAGG
ATACTGGAAGGGGAGTCTTA

Downstream 100 bases:

>100_bases
GTTTGACGAAAAAGGCTTGATTCCGGCGGTGGTACAGGATATTGATACCAGAGAAGTTTTAATGGTAGCTTACATGAATG
AAGAAAGCGTAAAAAAATCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLTVRIIPCLDVNKGRVVKGVNFLNLVDAGDPVELAAFYDREGADEVVFLDITASFEGRQTMVEVAKRTATTLSIPFTIG
GGIRNIDDIRALLASGADKVSINSAAVKNPELVREAALKFGSQCIVVAIDAKRKKDGGFEVYIHGGRTPTGIDAVEWAKE
VERLGAGEILLTSMDRDGTKDGYDLELTREIADAVKIPVIASGGVGELSHFYEGVVLGHASALLAASVFHFGKFTIKEVK
RYLKERGIPVRLGDTENGT

Sequences:

>Translated_259_residues
MLTVRIIPCLDVNKGRVVKGVNFLNLVDAGDPVELAAFYDREGADEVVFLDITASFEGRQTMVEVAKRTATTLSIPFTIG
GGIRNIDDIRALLASGADKVSINSAAVKNPELVREAALKFGSQCIVVAIDAKRKKDGGFEVYIHGGRTPTGIDAVEWAKE
VERLGAGEILLTSMDRDGTKDGYDLELTREIADAVKIPVIASGGVGELSHFYEGVVLGHASALLAASVFHFGKFTIKEVK
RYLKERGIPVRLGDTENGT
>Mature_259_residues
MLTVRIIPCLDVNKGRVVKGVNFLNLVDAGDPVELAAFYDREGADEVVFLDITASFEGRQTMVEVAKRTATTLSIPFTIG
GGIRNIDDIRALLASGADKVSINSAAVKNPELVREAALKFGSQCIVVAIDAKRKKDGGFEVYIHGGRTPTGIDAVEWAKE
VERLGAGEILLTSMDRDGTKDGYDLELTREIADAVKIPVIASGGVGELSHFYEGVVLGHASALLAASVFHFGKFTIKEVK
RYLKERGIPVRLGDTENGT

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.136186770428, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082028, Length=250, Percent_Identity=28, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=37.1428571428571, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CARHZ (Q3AD48)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359936.1
- ProteinModelPortal:   Q3AD48
- SMR:   Q3AD48
- STRING:   Q3AD48
- GeneID:   3727968
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1090
- NMPDR:   fig|246194.3.peg.1137
- TIGR:   CHY_1090
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   CHYD246194:CHY_1090-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27833; Mature: 27833

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVRIIPCLDVNKGRVVKGVNFLNLVDAGDPVELAAFYDREGADEVVFLDITASFEGRQ
CEEEEEEEEEECCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCCCHH
TMVEVAKRTATTLSIPFTIGGGIRNIDDIRALLASGADKVSINSAAVKNPELVREAALKF
HHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHC
GSQCIVVAIDAKRKKDGGFEVYIHGGRTPTGIDAVEWAKEVERLGAGEILLTSMDRDGTK
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGYDLELTREIADAVKIPVIASGGVGELSHFYEGVVLGHASALLAASVFHFGKFTIKEVK
CCCCEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RYLKERGIPVRLGDTENGT
HHHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MLTVRIIPCLDVNKGRVVKGVNFLNLVDAGDPVELAAFYDREGADEVVFLDITASFEGRQ
CEEEEEEEEEECCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCCCHH
TMVEVAKRTATTLSIPFTIGGGIRNIDDIRALLASGADKVSINSAAVKNPELVREAALKF
HHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHC
GSQCIVVAIDAKRKKDGGFEVYIHGGRTPTGIDAVEWAKEVERLGAGEILLTSMDRDGTK
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGYDLELTREIADAVKIPVIASGGVGELSHFYEGVVLGHASALLAASVFHFGKFTIKEVK
CCCCEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RYLKERGIPVRLGDTENGT
HHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA