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The map label for this gene is hemC

Identifier: 78044433

GI number: 78044433

Start: 1074465

End: 1075400

Strand: Direct

Name: hemC

Synonym: CHY_1208

Alternate gene names: 78044433

Gene position: 1074465-1075400 (Clockwise)

Preceding gene: 78044730

Following gene: 78043231

Centisome position: 44.74

GC content: 42.31

Gene sequence:

>936_bases
ATGAAAAAAGAAGTTATTGTGGGGAGTCGAGATAGTGCTTTAGCTTTGTGGCAAACAAATTGGGTGGTGGAAAGACTAAG
CAAAATTCACCCAGAAATAAATTTTAAAATTGTGACCATGAAAACAAAAGGGGATAAAATCCTGGACGTAGCTCTGGCCA
AAATTGGGGACAAAGGGCTTTTCACTAAAGAATTAGAACATGCCTTGTTAAATAAAACCATAGATATGGCTGTCCATAGC
ATGAAAGACTTACCAACAGTTTTGCCCGAAGGCTTGAAAATTGGTGCGTTTTGTGAAAGGGAGTACCCGGGAGATGTTTT
TATTTCCCCCAAGGGTTTTAAGTTTTTGGAACTACCTCAGGGGGCGAGAATTGGTACCAGCAGTTTGCGGCGGATAGCCC
AAATTTTAGCCGTAAGGCCGGATTTACAAGCCATACCGCTGCGCGGAAACCTACCTACACGTTTTCGTAAAATGGAGGAA
ATGGACTTAGATGGCATTATCTTGGCTTATGCCGGTGTGAAAAGATTGGGTTATGAGGATAAAATTACCGAGATGTTAAG
TTTTGACCTCTGTTTACCGGCGGTTGGTCAAGGTTCGATAGGTGTGGAAATCCGTGCCGATGATACTTTTATCGAAGAGC
TTTTAAAACCAATCGATCATTTTGCAACAAACCGAGCTATTAGAGCAGAAAGGGCCTTCTTAAAGCGCTTGGAAGGAGGA
TGTCAAATCCCCATTGGCGCCTATTCTGAAGTTAAAGAAAACCGACTTCATTTGAAAGGCGTTGTTGCCAGTTTAGATGG
GAAGTTAGTTATTAAAGGTGAAAAAGAGGGAAGTATTGAGGAACCGGAGAAAGTGGGGATTAGTTTAGCGGAGGAACTTT
TAACGAAAGGCGCCCAAAAAATCTTAGAGGAAATCAGGCGGGATGCCAATGAGTAA

Upstream 100 bases:

>100_bases
CATTTGTACACCGAAATTTTGCAAAACTTATTTGATTTACAAGTTGAAGGGGAACGCCCGAAAAATTTCACCCATCCGAG
AGAAGAAATGGAGGAAAGTG

Downstream 100 bases:

>100_bases
TTCGCTTACCTGCCGGGTTTTGGTAGGGGAAAAAGTTGATTACTTTTTAAATTCGGAATTGTTTTTTCGGGGATTACGGG
ACGCAAAAAATATTTATTTT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKKEVIVGSRDSALALWQTNWVVERLSKIHPEINFKIVTMKTKGDKILDVALAKIGDKGLFTKELEHALLNKTIDMAVHS
MKDLPTVLPEGLKIGAFCEREYPGDVFISPKGFKFLELPQGARIGTSSLRRIAQILAVRPDLQAIPLRGNLPTRFRKMEE
MDLDGIILAYAGVKRLGYEDKITEMLSFDLCLPAVGQGSIGVEIRADDTFIEELLKPIDHFATNRAIRAERAFLKRLEGG
CQIPIGAYSEVKENRLHLKGVVASLDGKLVIKGEKEGSIEEPEKVGISLAEELLTKGAQKILEEIRRDANE

Sequences:

>Translated_311_residues
MKKEVIVGSRDSALALWQTNWVVERLSKIHPEINFKIVTMKTKGDKILDVALAKIGDKGLFTKELEHALLNKTIDMAVHS
MKDLPTVLPEGLKIGAFCEREYPGDVFISPKGFKFLELPQGARIGTSSLRRIAQILAVRPDLQAIPLRGNLPTRFRKMEE
MDLDGIILAYAGVKRLGYEDKITEMLSFDLCLPAVGQGSIGVEIRADDTFIEELLKPIDHFATNRAIRAERAFLKRLEGG
CQIPIGAYSEVKENRLHLKGVVASLDGKLVIKGEKEGSIEEPEKVGISLAEELLTKGAQKILEEIRRDANE
>Mature_311_residues
MKKEVIVGSRDSALALWQTNWVVERLSKIHPEINFKIVTMKTKGDKILDVALAKIGDKGLFTKELEHALLNKTIDMAVHS
MKDLPTVLPEGLKIGAFCEREYPGDVFISPKGFKFLELPQGARIGTSSLRRIAQILAVRPDLQAIPLRGNLPTRFRKMEE
MDLDGIILAYAGVKRLGYEDKITEMLSFDLCLPAVGQGSIGVEIRADDTFIEELLKPIDHFATNRAIRAERAFLKRLEGG
CQIPIGAYSEVKENRLHLKGVVASLDGKLVIKGEKEGSIEEPEKVGISLAEELLTKGAQKILEEIRRDANE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=334, Percent_Identity=43.7125748502994, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI66933009, Length=334, Percent_Identity=43.7125748502994, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI48994974, Length=299, Percent_Identity=48.8294314381271, Blast_Score=288, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6319996, Length=317, Percent_Identity=38.801261829653, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20130425, Length=324, Percent_Identity=41.9753086419753, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CARHZ (Q3ACT3)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_360051.1
- HSSP:   P06983
- ProteinModelPortal:   Q3ACT3
- SMR:   Q3ACT3
- STRING:   Q3ACT3
- GeneID:   3727551
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1208
- NMPDR:   fig|246194.3.peg.1257
- TIGR:   CHY_1208
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   CHYD246194:CHY_1208-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34595; Mature: 34595

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEVIVGSRDSALALWQTNWVVERLSKIHPEINFKIVTMKTKGDKILDVALAKIGDKGL
CCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCC
FTKELEHALLNKTIDMAVHSMKDLPTVLPEGLKIGAFCEREYPGDVFISPKGFKFLELPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEECCCCCCCEEECCCCCEEEECCC
GARIGTSSLRRIAQILAVRPDLQAIPLRGNLPTRFRKMEEMDLDGIILAYAGVKRLGYED
CCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHCCCHH
KITEMLSFDLCLPAVGQGSIGVEIRADDTFIEELLKPIDHFATNRAIRAERAFLKRLEGG
HHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
CQIPIGAYSEVKENRLHLKGVVASLDGKLVIKGEKEGSIEEPEKVGISLAEELLTKGAQK
CCCCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
ILEEIRRDANE
HHHHHHHHCCC
>Mature Secondary Structure
MKKEVIVGSRDSALALWQTNWVVERLSKIHPEINFKIVTMKTKGDKILDVALAKIGDKGL
CCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCC
FTKELEHALLNKTIDMAVHSMKDLPTVLPEGLKIGAFCEREYPGDVFISPKGFKFLELPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEECCCCCCCEEECCCCCEEEECCC
GARIGTSSLRRIAQILAVRPDLQAIPLRGNLPTRFRKMEEMDLDGIILAYAGVKRLGYED
CCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHCCCHH
KITEMLSFDLCLPAVGQGSIGVEIRADDTFIEELLKPIDHFATNRAIRAERAFLKRLEGG
HHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
CQIPIGAYSEVKENRLHLKGVVASLDGKLVIKGEKEGSIEEPEKVGISLAEELLTKGAQK
CCCCCCCHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
ILEEIRRDANE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA