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The map label for this gene is selU [H]

Identifier: 78044383

GI number: 78044383

Start: 1036110

End: 1037150

Strand: Direct

Name: selU [H]

Synonym: CHY_1162

Alternate gene names: 78044383

Gene position: 1036110-1037150 (Clockwise)

Preceding gene: 78043722

Following gene: 78044924

Centisome position: 43.14

GC content: 36.5

Gene sequence:

>1041_bases
ATGAAAAGCGTAACTTTTTCCGACGTAAAAAATTTAAAAAATCCGGTGTTTGTTGATGTCCGTTCTCCTAAAGAGTTTAT
TGAGGATCACATTCCCGGCGCCCTGAATTTACCGCTTTTTAGTGATTGGGAAAGGGAAGTTATAGGTAAAATATATAAAT
TTCAAGGCGTAAGCCAGGCAAAATTAGCAGGATTAAATTTTTTATCTCCAAAGCTTCCCCGCATGGTGAATGAAATCCTT
AAATATAAAGAACAGGGAGATGTCGTAATTTATTGCTGGCGGGGAGGTTTAAGAAGTTTTGTTTTGGCAGAAATTCTGAG
GATGGTTGATATCTATGTTTACCGTTTAGAAGGGGGCTATAAAAGTTACCGCAGAGAAGTAGTTAAATTCTTTGAAACAG
TTGAAAATTTGCATCCTATTGTTCTCTACGGAATGACCGGGGTTGGGAAAACAAAAATTTTAGAAAACTTACAACGGCAA
GGATTACCGGTTTTAAACTTAGAAAAACTTGCCAATCACCGGGGTTCGGTTTTTGGACATTTGGGGCTTGAGCCCCAGCC
CTCCCAAAAATTTTTTGAATCTAAATTATACGAAGTTTTAAAGGATTTGCAGGGAATCCCCTTTTTAACGGAAGGAGAAA
GCCAAAAGATCGGAAAACTTTTTATTCCCAGAGGATTGTTTAAAAAGATGCAGAAAGCACCGGTGGTATTGTTAGAATTG
CCGGAAGAAAAGCGTATTGAAAACCTCATGTTTGAATATAAAGCGGAAAAAATTGATATTGAATTGTTTATAAAAGCTTT
GCAAAGTATTACACCCTATTTGGGCAGAGCAAAGGTGGAAAAGCTTATAGAAGATTTAAGAATGCGAAGATTTTATGATT
TTACTAAGACTCTGCTTCTTGACTATTATGACCCTCTTTATAAAAAATCCACGGCCTATTTACAAAATATAAAAAAGGTT
ATTTCCGGAAAAGATATTGATGAACTAACCGCAAAAGTAGGGGAATTTTGGAGGGAAGTGGCAGGAGGGAAACAAAAATG
A

Upstream 100 bases:

>100_bases
ATAATTCACCGGGCGGATGCCCGGTTTTTATTATTGACCGGAAAAATTGCCGGTGTTATACTTAATCGAGACTCTTTCAT
TAGCTTACGGGTGATACTCC

Downstream 100 bases:

>100_bases
TTGGGGAAAAATTAAGAAAACGCCGGGAACAATTAGGTTTAACCATAAAACAAGTGGAAGAAGAGATTAAGATCCGTAGT
AAGTATCTACAAGCTTTGGA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKSVTFSDVKNLKNPVFVDVRSPKEFIEDHIPGALNLPLFSDWEREVIGKIYKFQGVSQAKLAGLNFLSPKLPRMVNEIL
KYKEQGDVVIYCWRGGLRSFVLAEILRMVDIYVYRLEGGYKSYRREVVKFFETVENLHPIVLYGMTGVGKTKILENLQRQ
GLPVLNLEKLANHRGSVFGHLGLEPQPSQKFFESKLYEVLKDLQGIPFLTEGESQKIGKLFIPRGLFKKMQKAPVVLLEL
PEEKRIENLMFEYKAEKIDIELFIKALQSITPYLGRAKVEKLIEDLRMRRFYDFTKTLLLDYYDPLYKKSTAYLQNIKKV
ISGKDIDELTAKVGEFWREVAGGKQK

Sequences:

>Translated_346_residues
MKSVTFSDVKNLKNPVFVDVRSPKEFIEDHIPGALNLPLFSDWEREVIGKIYKFQGVSQAKLAGLNFLSPKLPRMVNEIL
KYKEQGDVVIYCWRGGLRSFVLAEILRMVDIYVYRLEGGYKSYRREVVKFFETVENLHPIVLYGMTGVGKTKILENLQRQ
GLPVLNLEKLANHRGSVFGHLGLEPQPSQKFFESKLYEVLKDLQGIPFLTEGESQKIGKLFIPRGLFKKMQKAPVVLLEL
PEEKRIENLMFEYKAEKIDIELFIKALQSITPYLGRAKVEKLIEDLRMRRFYDFTKTLLLDYYDPLYKKSTAYLQNIKKV
ISGKDIDELTAKVGEFWREVAGGKQK
>Mature_346_residues
MKSVTFSDVKNLKNPVFVDVRSPKEFIEDHIPGALNLPLFSDWEREVIGKIYKFQGVSQAKLAGLNFLSPKLPRMVNEIL
KYKEQGDVVIYCWRGGLRSFVLAEILRMVDIYVYRLEGGYKSYRREVVKFFETVENLHPIVLYGMTGVGKTKILENLQRQ
GLPVLNLEKLANHRGSVFGHLGLEPQPSQKFFESKLYEVLKDLQGIPFLTEGESQKIGKLFIPRGLFKKMQKAPVVLLEL
PEEKRIENLMFEYKAEKIDIELFIKALQSITPYLGRAKVEKLIEDLRMRRFYDFTKTLLLDYYDPLYKKSTAYLQNIKKV
ISGKDIDELTAKVGEFWREVAGGKQK

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=326, Percent_Identity=32.2085889570552, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40085; Mature: 40085

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVTFSDVKNLKNPVFVDVRSPKEFIEDHIPGALNLPLFSDWEREVIGKIYKFQGVSQA
CCCCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
KLAGLNFLSPKLPRMVNEILKYKEQGDVVIYCWRGGLRSFVLAEILRMVDIYVYRLEGGY
HHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCH
KSYRREVVKFFETVENLHPIVLYGMTGVGKTKILENLQRQGLPVLNLEKLANHRGSVFGH
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEECHHHHHCCCCCEEEE
LGLEPQPSQKFFESKLYEVLKDLQGIPFLTEGESQKIGKLFIPRGLFKKMQKAPVVLLEL
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCHHHHHHHHHCCEEEEEC
PEEKRIENLMFEYKAEKIDIELFIKALQSITPYLGRAKVEKLIEDLRMRRFYDFTKTLLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYYDPLYKKSTAYLQNIKKVISGKDIDELTAKVGEFWREVAGGKQK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSVTFSDVKNLKNPVFVDVRSPKEFIEDHIPGALNLPLFSDWEREVIGKIYKFQGVSQA
CCCCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
KLAGLNFLSPKLPRMVNEILKYKEQGDVVIYCWRGGLRSFVLAEILRMVDIYVYRLEGGY
HHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCH
KSYRREVVKFFETVENLHPIVLYGMTGVGKTKILENLQRQGLPVLNLEKLANHRGSVFGH
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEECHHHHHCCCCCEEEE
LGLEPQPSQKFFESKLYEVLKDLQGIPFLTEGESQKIGKLFIPRGLFKKMQKAPVVLLEL
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCHHHHHHHHHCCEEEEEC
PEEKRIENLMFEYKAEKIDIELFIKALQSITPYLGRAKVEKLIEDLRMRRFYDFTKTLLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYYDPLYKKSTAYLQNIKKVISGKDIDELTAKVGEFWREVAGGKQK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA