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The map label for this gene is vipB [H]

Identifier: 78044276

GI number: 78044276

Start: 934570

End: 935511

Strand: Direct

Name: vipB [H]

Synonym: CHY_1057

Alternate gene names: 78044276

Gene position: 934570-935511 (Clockwise)

Preceding gene: 78043529

Following gene: 78043838

Centisome position: 38.92

GC content: 44.8

Gene sequence:

>942_bases
TTGGCAAAATTCCTGGTTACCGGTGGAGCAGGTTTTATTGGCTCGCACATAGTGGAAAGGTTGGTACGAGACGGAGCTGA
GGTCGTTGTCCTGGATGATTTATCAAGCGGTAAAGAGGAAAATCTCTCGGAAGTTTTAGATAAAATTACCTTTATTAAAG
GAGATGTCCGGGATTTAGATTTGATAAAAGGAATTACTAAAGATGTGGATTATATTTTGCACGAAGCGGCGATGGCGTCG
GTGCCGGCTTCTATCGATGACCCTTTAAAATGCCATGAAGTAAACGTTACCGGGACGATTAATGTGCTTTTATCTGCTAA
AGAAAACGGAGTAAAAAGAGTGGTTTACGCGGCATCTTCGGCAGTATACGGGAATAATGAAACTTTACCCAAGAAAGAAG
ATATGTACCCGGAGCCGCTTTCTCCCTATGCGGTTTCGAAGTATGCCGGAGAGTTATATCTTCAAGTTTTTGCCCGGATT
TACGGTATTGAAGCGGTGGGACTTCGGTACTTTAATGTTTTTGGGCCAAAGCAGGATCCCAATTCCCAGTACGCAGCGGT
GATACCTAAGTTTATCGATGCATTGTTAAAAGGTATGCCGCCGACAATTTACGGGGATGGAATGCAGACCAGGGATTTTA
TTTTTATCGATGATGTGGTGGAGGCAAATATGCTGGCTTTAACGGCCCGGGGGGCTTCAGGGAAGGTATTTAATATTGCC
TGCGGAGAGCGGATTAGTTTAAATAGGCTTTACAAAGTGATAAAAGAGATTATCGGGGTGGATATTGAGCCGGTATATGC
TGAGGCAAGGGTTGGAGATGTCCGGGACTCCCTGGCGGATATATCTTTGGCCCGGAATATTTTGGGGTTTGAGCCAAAGG
TGAGTTTGGAGGAGGGCCTTAAAAAGACGGTTGAGTGGCATAAACGGAATTTGGAGGTTTGA

Upstream 100 bases:

>100_bases
CTGTTTTAAAATTCATTTTAAACCTCCGTGTAGTGAAAAAATGTACCAAATTTAGTATAATGTTTGTAGTTTAAAACTGT
AAAGAGAAAGGGGATTAACT

Downstream 100 bases:

>100_bases
AAGTAGAGGGTGGATGCTGGAGAATTAATATGAAGAAGAAAATAAAACTTTTACACCTCATTACCTTAAGTGAGGCGGGA
GGAGCCCAGAAGGTTCTCTA

Product: hypothetical protein

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAKFLVTGGAGFIGSHIVERLVRDGAEVVVLDDLSSGKEENLSEVLDKITFIKGDVRDLDLIKGITKDVDYILHEAAMAS
VPASIDDPLKCHEVNVTGTINVLLSAKENGVKRVVYAASSAVYGNNETLPKKEDMYPEPLSPYAVSKYAGELYLQVFARI
YGIEAVGLRYFNVFGPKQDPNSQYAAVIPKFIDALLKGMPPTIYGDGMQTRDFIFIDDVVEANMLALTARGASGKVFNIA
CGERISLNRLYKVIKEIIGVDIEPVYAEARVGDVRDSLADISLARNILGFEPKVSLEEGLKKTVEWHKRNLEV

Sequences:

>Translated_313_residues
MAKFLVTGGAGFIGSHIVERLVRDGAEVVVLDDLSSGKEENLSEVLDKITFIKGDVRDLDLIKGITKDVDYILHEAAMAS
VPASIDDPLKCHEVNVTGTINVLLSAKENGVKRVVYAASSAVYGNNETLPKKEDMYPEPLSPYAVSKYAGELYLQVFARI
YGIEAVGLRYFNVFGPKQDPNSQYAAVIPKFIDALLKGMPPTIYGDGMQTRDFIFIDDVVEANMLALTARGASGKVFNIA
CGERISLNRLYKVIKEIIGVDIEPVYAEARVGDVRDSLADISLARNILGFEPKVSLEEGLKKTVEWHKRNLEV
>Mature_312_residues
AKFLVTGGAGFIGSHIVERLVRDGAEVVVLDDLSSGKEENLSEVLDKITFIKGDVRDLDLIKGITKDVDYILHEAAMASV
PASIDDPLKCHEVNVTGTINVLLSAKENGVKRVVYAASSAVYGNNETLPKKEDMYPEPLSPYAVSKYAGELYLQVFARIY
GIEAVGLRYFNVFGPKQDPNSQYAAVIPKFIDALLKGMPPTIYGDGMQTRDFIFIDDVVEANMLALTARGASGKVFNIAC
GERISLNRLYKVIKEIIGVDIEPVYAEARVGDVRDSLADISLARNILGFEPKVSLEEGLKKTVEWHKRNLEV

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=33.6507936507937, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=34.3653250773994, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI56237023, Length=338, Percent_Identity=28.698224852071, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI56118217, Length=338, Percent_Identity=28.698224852071, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI189083684, Length=338, Percent_Identity=28.698224852071, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI193211614, Length=341, Percent_Identity=24.3401759530792, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8393516, Length=341, Percent_Identity=24.3401759530792, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI116268111, Length=278, Percent_Identity=26.2589928057554, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=27.9761904761905, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=29.8245614035088, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790049, Length=252, Percent_Identity=30.1587301587302, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788589, Length=348, Percent_Identity=25.2873563218391, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788365, Length=228, Percent_Identity=24.1228070175439, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=23.342939481268, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=313, Percent_Identity=32.2683706070287, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=28.9230769230769, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=28.1065088757396, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=340, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=27.3846153846154, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17570557, Length=190, Percent_Identity=30, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=341, Percent_Identity=26.6862170087977, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321437, Length=352, Percent_Identity=23.5795454545455, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356223, Length=314, Percent_Identity=32.1656050955414, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI19923002, Length=340, Percent_Identity=31.7647058823529, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 34359; Mature: 34228

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFLVTGGAGFIGSHIVERLVRDGAEVVVLDDLSSGKEENLSEVLDKITFIKGDVRDLD
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
LIKGITKDVDYILHEAAMASVPASIDDPLKCHEVNVTGTINVLLSAKENGVKRVVYAASS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEEEHHH
AVYGNNETLPKKEDMYPEPLSPYAVSKYAGELYLQVFARIYGIEAVGLRYFNVFGPKQDP
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEECCCCCCC
NSQYAAVIPKFIDALLKGMPPTIYGDGMQTRDFIFIDDVVEANMLALTARGASGKVFNIA
CCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEHHHHCCCEEEEEECCCCCCEEEEE
CGERISLNRLYKVIKEIIGVDIEPVYAEARVGDVRDSLADISLARNILGFEPKVSLEEGL
CCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KKTVEWHKRNLEV
HHHHHHHHHCCCC
>Mature Secondary Structure 
AKFLVTGGAGFIGSHIVERLVRDGAEVVVLDDLSSGKEENLSEVLDKITFIKGDVRDLD
CEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
LIKGITKDVDYILHEAAMASVPASIDDPLKCHEVNVTGTINVLLSAKENGVKRVVYAASS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEEEHHH
AVYGNNETLPKKEDMYPEPLSPYAVSKYAGELYLQVFARIYGIEAVGLRYFNVFGPKQDP
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEECCCCCCC
NSQYAAVIPKFIDALLKGMPPTIYGDGMQTRDFIFIDDVVEANMLALTARGASGKVFNIA
CCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEHHHHCCCEEEEEECCCCCCEEEEE
CGERISLNRLYKVIKEIIGVDIEPVYAEARVGDVRDSLADISLARNILGFEPKVSLEEGL
CCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KKTVEWHKRNLEV
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]