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The map label for this gene is wcaJ [H]

Identifier: 78043113

GI number: 78043113

Start: 936691

End: 938019

Strand: Direct

Name: wcaJ [H]

Synonym: CHY_1059

Alternate gene names: 78043113

Gene position: 936691-938019 (Clockwise)

Preceding gene: 78043838

Following gene: 78043940

Centisome position: 39.0

GC content: 43.57

Gene sequence:

>1329_bases
ATGGATAAAAATACAAAACTAAAACTTTTAACCATTGCTTCCGTTTTAATTGACGCCGTTTTCTTAAACCTCGGCTTTGT
AGCGGCTTTTTTGCTGCGCTTTGGTTTTAAGCTTCCTGAAGAAAACTTTATAGCTTATCTTACCGTTTACCCGTGGGTAA
CTTTAGCGGGGCTTTTACTTTTTAATGTGTATGGTTTATACCAGAGTTATAAACGAAGCCGGGCAGAAGTTATCGCCTCT
ACCGTTTGGGCTATTGTGCTTTTGCTGCCGGTAGCCATGGGCATGGCGTTCTTCTTCCGGGGTTTTGCCTTTCCGCGGAC
GGTTTTCGTGGTTGCCCTTATCCTGCAGCTTATAATTTTTATTCCCTGGCGGCTCTGGCTCTGGCGGCTGGAGAAAAAAG
TTTTTGGAGCAAGGCGGCTTTTATTAATTGGCGGTCATAAAGAAATCCACATTACCCGGGATAAGATCAAAGATAACAAG
ATTGACCTCTTTGAGGTTGCGGGAAAGCTGGCAGCTCCGGGAACAATTGAGGAAGTTAAGGAAGCCATCAATAAGTACCG
GCCGGAAGTGGTACTTATCGGTAATAACCTTCCGGACAAGCTTAAAAAAGAAATATTACTTTTAACTTTGTCGGAAAATA
TTCCGGTGTACGTTATCCCAGATTATTATGATATCGTCCTTTCCCAGGCCAAGCTTGAGTCGATAGGCGACTTACCGGTC
TTTTATTTGGAAGGGCTTTCCTTGCCTGCCGGGATGCAGATGGTCAAAAGGGCGATGGATATCTTGCTGGCTTTAATTGC
CCTAATTTTGGCAAGCCCGATAATGCTTGTAACTGCCCTTTTAATTAAGCTTGATTCCGAAGGACCGGTATTTTACTTAC
AGGAAAGGGTTACGGAAAACGGGCGAAAATTTTACTGTTATAAATTCCGCACCATGGTGAAAGATGCGGAGAAACTCACG
GGACCTACTTTAGCTACCGAAAACGATCCCCGGATTACCCGGGTAGGAAGGTTTTTACGGGCAACCAGGATTGATGAATT
GCCCCAGTTAATAAATGTTTTAAAGGGAGACATGAGCTTAGTTGGACCAAGGCCCGAACGTCCTTTCTTTGTCGAAAAAT
TTACCGATGAGGTGCCGGAGTACGCCTTACGGCTAAAACTAAAATCCGGCCTAACCGGTCTTGCCCAGGTTGAAGGGAAA
TACAGTACCTCTTTTGAAGACAAGCTAAAATACGACTTGCTGTACGCCAAAGCTTATACCCCTCTAAAAGATTTAGAAAT
TATCCTGCACACGTTGAAAGTTATTTTAATTAAGGATAAAGCATCTTGA

Upstream 100 bases:

>100_bases
AGTTTTTTTCTTTATAGCGAAAATTATACCGTTGAAAAAATGGTCAGTTCCTATGACTGCCTGTACAAGAAACTTTTAAA
AGATACGGAGGCAGTTTTTG

Downstream 100 bases:

>100_bases
ATGGAAAAAGATATTAAACCTCCCATAAAATGGGAGGTTTTTTTCTGTTTTTAGCGAATATTATTTTATTAGTAAAAACT
AGTAAAAAAAGGGAGGGTTA

Product: sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDKNTKLKLLTIASVLIDAVFLNLGFVAAFLLRFGFKLPEENFIAYLTVYPWVTLAGLLLFNVYGLYQSYKRSRAEVIAS
TVWAIVLLLPVAMGMAFFFRGFAFPRTVFVVALILQLIIFIPWRLWLWRLEKKVFGARRLLLIGGHKEIHITRDKIKDNK
IDLFEVAGKLAAPGTIEEVKEAINKYRPEVVLIGNNLPDKLKKEILLLTLSENIPVYVIPDYYDIVLSQAKLESIGDLPV
FYLEGLSLPAGMQMVKRAMDILLALIALILASPIMLVTALLIKLDSEGPVFYLQERVTENGRKFYCYKFRTMVKDAEKLT
GPTLATENDPRITRVGRFLRATRIDELPQLINVLKGDMSLVGPRPERPFFVEKFTDEVPEYALRLKLKSGLTGLAQVEGK
YSTSFEDKLKYDLLYAKAYTPLKDLEIILHTLKVILIKDKAS

Sequences:

>Translated_442_residues
MDKNTKLKLLTIASVLIDAVFLNLGFVAAFLLRFGFKLPEENFIAYLTVYPWVTLAGLLLFNVYGLYQSYKRSRAEVIAS
TVWAIVLLLPVAMGMAFFFRGFAFPRTVFVVALILQLIIFIPWRLWLWRLEKKVFGARRLLLIGGHKEIHITRDKIKDNK
IDLFEVAGKLAAPGTIEEVKEAINKYRPEVVLIGNNLPDKLKKEILLLTLSENIPVYVIPDYYDIVLSQAKLESIGDLPV
FYLEGLSLPAGMQMVKRAMDILLALIALILASPIMLVTALLIKLDSEGPVFYLQERVTENGRKFYCYKFRTMVKDAEKLT
GPTLATENDPRITRVGRFLRATRIDELPQLINVLKGDMSLVGPRPERPFFVEKFTDEVPEYALRLKLKSGLTGLAQVEGK
YSTSFEDKLKYDLLYAKAYTPLKDLEIILHTLKVILIKDKAS
>Mature_442_residues
MDKNTKLKLLTIASVLIDAVFLNLGFVAAFLLRFGFKLPEENFIAYLTVYPWVTLAGLLLFNVYGLYQSYKRSRAEVIAS
TVWAIVLLLPVAMGMAFFFRGFAFPRTVFVVALILQLIIFIPWRLWLWRLEKKVFGARRLLLIGGHKEIHITRDKIKDNK
IDLFEVAGKLAAPGTIEEVKEAINKYRPEVVLIGNNLPDKLKKEILLLTLSENIPVYVIPDYYDIVLSQAKLESIGDLPV
FYLEGLSLPAGMQMVKRAMDILLALIALILASPIMLVTALLIKLDSEGPVFYLQERVTENGRKFYCYKFRTMVKDAEKLT
GPTLATENDPRITRVGRFLRATRIDELPQLINVLKGDMSLVGPRPERPFFVEKFTDEVPEYALRLKLKSGLTGLAQVEGK
YSTSFEDKLKYDLLYAKAYTPLKDLEIILHTLKVILIKDKAS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=420, Percent_Identity=30, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 50270; Mature: 50270

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNTKLKLLTIASVLIDAVFLNLGFVAAFLLRFGFKLPEENFIAYLTVYPWVTLAGLLL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
FNVYGLYQSYKRSRAEVIASTVWAIVLLLPVAMGMAFFFRGFAFPRTVFVVALILQLIIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IPWRLWLWRLEKKVFGARRLLLIGGHKEIHITRDKIKDNKIDLFEVAGKLAAPGTIEEVK
HHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCHHHHH
EAINKYRPEVVLIGNNLPDKLKKEILLLTLSENIPVYVIPDYYDIVLSQAKLESIGDLPV
HHHHHHCCEEEEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCHH
FYLEGLSLPAGMQMVKRAMDILLALIALILASPIMLVTALLIKLDSEGPVFYLQERVTEN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCC
GRKFYCYKFRTMVKDAEKLTGPTLATENDPRITRVGRFLRATRIDELPQLINVLKGDMSL
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VGPRPERPFFVEKFTDEVPEYALRLKLKSGLTGLAQVEGKYSTSFEDKLKYDLLYAKAYT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHEEHHHCC
PLKDLEIILHTLKVILIKDKAS
CHHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure
MDKNTKLKLLTIASVLIDAVFLNLGFVAAFLLRFGFKLPEENFIAYLTVYPWVTLAGLLL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
FNVYGLYQSYKRSRAEVIASTVWAIVLLLPVAMGMAFFFRGFAFPRTVFVVALILQLIIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IPWRLWLWRLEKKVFGARRLLLIGGHKEIHITRDKIKDNKIDLFEVAGKLAAPGTIEEVK
HHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCHHHHH
EAINKYRPEVVLIGNNLPDKLKKEILLLTLSENIPVYVIPDYYDIVLSQAKLESIGDLPV
HHHHHHCCEEEEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCHH
FYLEGLSLPAGMQMVKRAMDILLALIALILASPIMLVTALLIKLDSEGPVFYLQERVTEN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCC
GRKFYCYKFRTMVKDAEKLTGPTLATENDPRITRVGRFLRATRIDELPQLINVLKGDMSL
CCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VGPRPERPFFVEKFTDEVPEYALRLKLKSGLTGLAQVEGKYSTSFEDKLKYDLLYAKAYT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHEEHHHCC
PLKDLEIILHTLKVILIKDKAS
CHHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]