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The map label for this gene is ppsB [H]

Identifier: 77747687

GI number: 77747687

Start: 1538464

End: 1540110

Strand: Reverse

Name: ppsB [H]

Synonym: PD1311

Alternate gene names: 77747687

Gene position: 1540110-1538464 (Counterclockwise)

Preceding gene: 28199198

Following gene: 28199191

Centisome position: 61.12

GC content: 56.53

Gene sequence:

>1647_bases
ATGAAAGAGCCTTGTAACATCGCTGCTGCGCTGCCGCGTTTGGCATCCGAACGCCCCGAGCAGATTGCGATTCGCTGTCC
GGGGCGTCCCGGTTCTGGCGGGATGGCGGCTTATGATTTAACCCTCAGCTACCGTGAATTAGACAGCCGCAGTGATGCGA
TTGCGGCTGGTTTCATCGCTTATGGTATCGGTCGTGGTGTGCGTACGGTGGTTATGGTGCGGCCCTCACCGGAATTTTTC
CTGCTGATGTTTGCCCTATTCAAGGTGGGCGCCGTACCGGTACTCGTGGATCCGGGCATTGATCGATCGGCCTTGAAACA
ATGCTTGAGCGAGGCTGAACCTCAGGCATTCATCGGTGTTTTTCTGGCCCAACTGGCGCGGCGTTTATTGCGTTGGGCGC
CTTCTGTCACCCGCGTTGTCAGTGTCGGTACCCGTTGGGCATGGTGTGGCACCACGCTGGCGCACCTGGAGCGTGCTGGC
ATAGGTGCCAGCGCACAACTGGCCGATACGACTGCCGACGAGGTGGCCGCGATTTTGTTTACCAGTGGCTCGACAGGCGT
GCCTAAAGGAGTGGTGTATCGTCACCGGCATTTTGTCGGTCAGATTGCGTTGTTGCGGGAAACGCTTGGCATTGTGCCCG
GAGGGGTAGATTTGCCGACCTTTCCTCCGTTTGCGTTATTTAATCCAGCACTTGGTTTAACCAGCATCATTCCGGATATG
GATCCCAGGTGCCCAGCCAGGGCTGATCCACGCAAGCTGCATGATGCCATCGCGCGTTTCGGGGTGAATCATTTATTCGG
TTCACCGGCATTGCTGCGGGTCTTAGCTGAGTATGGCAAACCATTGCCAACCCTGCGTTGCGTGACCTCAGCAGGTGCCC
CAGTGCCTCCGTATGTAGTGGCACAGATACGCGCGCTATTGTCGGATGAGGCGCAATTTTTGACACCGTATGGAGCGACC
GAATGTTTACCAATTGCGGTGATTGAGAGTCGTGAATTAGAAACGACGCGTGCTGCGACGGAGCGTGGTGCAGGGACGTG
CGTTGGTCATGTGATCGCACCTAATCTGGTACGTATCATCGCGATTGATGATGAGGGGATTGCAGAGTGGGGCGGGGCGC
GTGAAGTGGATTTGGGGGAGATTGGTGAAGTGACTGTCGTTGGTCCCAGTACCACCGATGCTTATTACAACCGTACAGCA
GCAACGCGCCTGGCTAAAATCCGCGAGCGTTTGGCTGATGGCAGTGAGCGTATCGTGCATCGCATGGGAGATCTGGGTTA
CTTTGATGCGCAGCGCCGTTTGTGGTTCTGTGGGCGCAAGACCCATCGTGTGGAGACAGTACAGGGACCGTTGTATACCG
AGCAGGTCGAGCCTGTTTTCAATGTTATTGATGGGGTGCGTCGCACTGCATTGGTGGGTATTGGTACGCTTGGCGCGCAG
CGCCCCGTATTGTGCTACGAGGCGCTGCCCGGTGTGGATGTGGCTGCGTTAGAACAGCGCTTGCGTGCCACGGCACGGGC
TTTTATACACACTGTTGGTATTGTCGATGTTCTAGGACATCCAGGGTTCCCGGTGGATATCCGTCATAACGCCAAAATTG
GACGTGAACGCTTGGCGGTTTGGGCAGCACAACGGTTGCGCTGCTGA

Upstream 100 bases:

>100_bases
ACAGGGGGAAAGGTATGCGGTGAGTATCAAACAACCCAGGCGTTTTGGGGATGTGATCGGCTGGAATGGACGCTGCTATT
CAGGGGGTGGAATTCAACAC

Downstream 100 bases:

>100_bases
TTGTTTTCAGTAGTGAGGAGTCGATTCTGTGACATCCTTGGCGGTGGTGCAATGTGGGTTGCGAGGATTGATCTTCAGCC
GTGATCAATGATGATCCCTA

Product: peptide synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Peptide synthase 2; ATP-dependent tyrosine adenylase 1; TyrA 1; Tyrosine activase 1; ATP-dependent threonine adenylase; ThrA; Threonine activase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKEPCNIAAALPRLASERPEQIAIRCPGRPGSGGMAAYDLTLSYRELDSRSDAIAAGFIAYGIGRGVRTVVMVRPSPEFF
LLMFALFKVGAVPVLVDPGIDRSALKQCLSEAEPQAFIGVFLAQLARRLLRWAPSVTRVVSVGTRWAWCGTTLAHLERAG
IGASAQLADTTADEVAAILFTSGSTGVPKGVVYRHRHFVGQIALLRETLGIVPGGVDLPTFPPFALFNPALGLTSIIPDM
DPRCPARADPRKLHDAIARFGVNHLFGSPALLRVLAEYGKPLPTLRCVTSAGAPVPPYVVAQIRALLSDEAQFLTPYGAT
ECLPIAVIESRELETTRAATERGAGTCVGHVIAPNLVRIIAIDDEGIAEWGGAREVDLGEIGEVTVVGPSTTDAYYNRTA
ATRLAKIRERLADGSERIVHRMGDLGYFDAQRRLWFCGRKTHRVETVQGPLYTEQVEPVFNVIDGVRRTALVGIGTLGAQ
RPVLCYEALPGVDVAALEQRLRATARAFIHTVGIVDVLGHPGFPVDIRHNAKIGRERLAVWAAQRLRC

Sequences:

>Translated_548_residues
MKEPCNIAAALPRLASERPEQIAIRCPGRPGSGGMAAYDLTLSYRELDSRSDAIAAGFIAYGIGRGVRTVVMVRPSPEFF
LLMFALFKVGAVPVLVDPGIDRSALKQCLSEAEPQAFIGVFLAQLARRLLRWAPSVTRVVSVGTRWAWCGTTLAHLERAG
IGASAQLADTTADEVAAILFTSGSTGVPKGVVYRHRHFVGQIALLRETLGIVPGGVDLPTFPPFALFNPALGLTSIIPDM
DPRCPARADPRKLHDAIARFGVNHLFGSPALLRVLAEYGKPLPTLRCVTSAGAPVPPYVVAQIRALLSDEAQFLTPYGAT
ECLPIAVIESRELETTRAATERGAGTCVGHVIAPNLVRIIAIDDEGIAEWGGAREVDLGEIGEVTVVGPSTTDAYYNRTA
ATRLAKIRERLADGSERIVHRMGDLGYFDAQRRLWFCGRKTHRVETVQGPLYTEQVEPVFNVIDGVRRTALVGIGTLGAQ
RPVLCYEALPGVDVAALEQRLRATARAFIHTVGIVDVLGHPGFPVDIRHNAKIGRERLAVWAAQRLRC
>Mature_548_residues
MKEPCNIAAALPRLASERPEQIAIRCPGRPGSGGMAAYDLTLSYRELDSRSDAIAAGFIAYGIGRGVRTVVMVRPSPEFF
LLMFALFKVGAVPVLVDPGIDRSALKQCLSEAEPQAFIGVFLAQLARRLLRWAPSVTRVVSVGTRWAWCGTTLAHLERAG
IGASAQLADTTADEVAAILFTSGSTGVPKGVVYRHRHFVGQIALLRETLGIVPGGVDLPTFPPFALFNPALGLTSIIPDM
DPRCPARADPRKLHDAIARFGVNHLFGSPALLRVLAEYGKPLPTLRCVTSAGAPVPPYVVAQIRALLSDEAQFLTPYGAT
ECLPIAVIESRELETTRAATERGAGTCVGHVIAPNLVRIIAIDDEGIAEWGGAREVDLGEIGEVTVVGPSTTDAYYNRTA
ATRLAKIRERLADGSERIVHRMGDLGYFDAQRRLWFCGRKTHRVETVQGPLYTEQVEPVFNVIDGVRRTALVGIGTLGAQ
RPVLCYEALPGVDVAALEQRLRATARAFIHTVGIVDVLGHPGFPVDIRHNAKIGRERLAVWAAQRLRC

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Tyr and Thr as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Thr residue is further converted to the D-allo-isomer form. The activation sit

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59145; Mature: 59145

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPCNIAAALPRLASERPEQIAIRCPGRPGSGGMAAYDLTLSYRELDSRSDAIAAGFIA
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHCCCCHHHHHHHHH
YGIGRGVRTVVMVRPSPEFFLLMFALFKVGAVPVLVDPGIDRSALKQCLSEAEPQAFIGV
HHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHH
FLAQLARRLLRWAPSVTRVVSVGTRWAWCGTTLAHLERAGIGASAQLADTTADEVAAILF
HHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHEEEEEE
TSGSTGVPKGVVYRHRHFVGQIALLRETLGIVPGGVDLPTFPPFALFNPALGLTSIIPDM
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCC
DPRCPARADPRKLHDAIARFGVNHLFGSPALLRVLAEYGKPLPTLRCVTSAGAPVPPYVV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHH
AQIRALLSDEAQFLTPYGATECLPIAVIESRELETTRAATERGAGTCVGHVIAPNLVRII
HHHHHHHCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEHHHHCCCEEEEE
AIDDEGIAEWGGAREVDLGEIGEVTVVGPSTTDAYYNRTAATRLAKIRERLADGSERIVH
EECCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RMGDLGYFDAQRRLWFCGRKTHRVETVQGPLYTEQVEPVFNVIDGVRRTALVGIGTLGAQ
HHCCCCCCHHCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCC
RPVLCYEALPGVDVAALEQRLRATARAFIHTVGIVDVLGHPGFPVDIRHNAKIGRERLAV
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
WAAQRLRC
HHHHHHCC
>Mature Secondary Structure
MKEPCNIAAALPRLASERPEQIAIRCPGRPGSGGMAAYDLTLSYRELDSRSDAIAAGFIA
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHCCCCHHHHHHHHH
YGIGRGVRTVVMVRPSPEFFLLMFALFKVGAVPVLVDPGIDRSALKQCLSEAEPQAFIGV
HHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHH
FLAQLARRLLRWAPSVTRVVSVGTRWAWCGTTLAHLERAGIGASAQLADTTADEVAAILF
HHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHEEEEEE
TSGSTGVPKGVVYRHRHFVGQIALLRETLGIVPGGVDLPTFPPFALFNPALGLTSIIPDM
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCC
DPRCPARADPRKLHDAIARFGVNHLFGSPALLRVLAEYGKPLPTLRCVTSAGAPVPPYVV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHH
AQIRALLSDEAQFLTPYGATECLPIAVIESRELETTRAATERGAGTCVGHVIAPNLVRII
HHHHHHHCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEHHHHCCCEEEEE
AIDDEGIAEWGGAREVDLGEIGEVTVVGPSTTDAYYNRTAATRLAKIRERLADGSERIVH
EECCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RMGDLGYFDAQRRLWFCGRKTHRVETVQGPLYTEQVEPVFNVIDGVRRTALVGIGTLGAQ
HHCCCCCCHHCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCC
RPVLCYEALPGVDVAALEQRLRATARAFIHTVGIVDVLGHPGFPVDIRHNAKIGRERLAV
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
WAAQRLRC
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377; 10471562 [H]