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The map label for this gene is yodT [H]

Identifier: 77465809

GI number: 77465809

Start: 925160

End: 926488

Strand: Reverse

Name: yodT [H]

Synonym: RSP_3803

Alternate gene names: 77465809

Gene position: 926488-925160 (Counterclockwise)

Preceding gene: 77465810

Following gene: 77465808

Centisome position: 98.25

GC content: 71.71

Gene sequence:

>1329_bases
ATGCCCGGACATATCTTCGGCCGCTCCACCCGGGGCAGCCTTCCCGTCGCCGCTGAGGGCGAGGGCTGCTACATCCTCGA
TACCGAGGGCCGGCGCTATCTCGACGGGTCGGGCGGGGCGGCGGTCTCCTGCCTCGGCCATTCCAACGCGGCGGTGCGCG
CGGCGCTCCACGACCAGCTGGACCGGCTGGCCTTCGCCCATACCGGCTTCTTCACCTCCGAACCGGCCGAGCGTCTGGCC
GACCGGCTGGCGGCGGCGGCGCCCGAGGGGATCGAGCGGGTCTATCTCGTCTCGGGCGGATCCGAGGCGGTCGAGGCGGC
GCTGAAGCTCGCGCGGCAGTATTTCATGGAAGTGGGCCAGCCCGGCCGGCACCGGGTGATCGCCCGCCGCCAGAGCTACC
ACGGCAACACGCTCGGCGCGCTGGCCGCAGGCGGCAACGAATGGCGCCGGGCCCAGTTCGCGCCGCTTCTGGTCGAGACC
TCCCATGTCTCGCCCTGCTACGAATATCGCGGCCGGGCCGAGGACGAGAGCCTCGAGGCCTACGGGCTGCGCGTGGCCGA
CGAGCTGGAGGCCGAGATCCAGCGCCTCGGTCCCGAGACGGTGATGGCCTTCGTGGCCGAGCCGGTGGTGGGGGCCACGG
CGGGCGCCGTGCCGGCCGTGCCGGGCTATCTCAAGCGCGTCCGCGAGATCTGCGACCGGCACGGGGTGCTGCTCATTCTC
GATGAGGTCATGTGCGGGATGGGCCGGACGGGCCATCTCTTCGCCTGCGCCGAAGATGGCGTGGCGCCCGACATGATCAC
CATCGCCAAGGGTCTCGGCGCGGGCTACCAGCCCATCGGCGCGCTTCTGGTTCAGGGCCGCATCTACGACGCCATCGCCG
CGGGCTCGGGCTTCTTCCAGCACGGCCACACCTACATGGGCCATGCGATGGCCGCCGCCGCGGCCAATGCGGTGCTGGAC
GAGATCGAGGGCCGCGATCTCCTGCAGGCGGTCCGGCGGCAGGGCGCGCGGCTCGAGGCGCTCCTGCAGGAGCGGCTGGG
CCAGCATCCCCATGTGGGCGACATCCGCGGCCGCGGTCTCTTCCGCGGCATCGAGCTGGTGGCCGACCGCGAGACGAAGG
AGCCGTTCGATCCGGCGCGGAAGCTCCATGCCCGGATCAAGGCCGAGGCCTTCGCAGGCGGCCTCATCTGCTATCCGATG
GGCGGCACCCTCGACGGGGTGCGGGGCGACCATATCCTGCTCGCGCCGCCCTTCATCATCTCGGACGCCGAACTCGACGA
GCTGGTGGACAAGCTCTCGGGCGCGCTCGACCGCGCCCTCGCGGCATGA

Upstream 100 bases:

>100_bases
GCGCACCGGCCCGAGTTCAGCGACTATCTCCTCGGCCCGAATGCCGCGCGCGTGGTGCGTCACGCCCGCCAATCCGTCTA
TGTCGTAAGGACCTGACCCG

Downstream 100 bases:

>100_bases
GCGGGCGCGACGGGGCGCCGGTCTCAGGGCGGCGCGGCTGATGGCGGTGCTGCCCCGGATCATGCTGGCGCCGACCGGCG
CGCGGCTCACCAAGGCCGAT

Product: hypothetical protein

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPGHIFGRSTRGSLPVAAEGEGCYILDTEGRRYLDGSGGAAVSCLGHSNAAVRAALHDQLDRLAFAHTGFFTSEPAERLA
DRLAAAAPEGIERVYLVSGGSEAVEAALKLARQYFMEVGQPGRHRVIARRQSYHGNTLGALAAGGNEWRRAQFAPLLVET
SHVSPCYEYRGRAEDESLEAYGLRVADELEAEIQRLGPETVMAFVAEPVVGATAGAVPAVPGYLKRVREICDRHGVLLIL
DEVMCGMGRTGHLFACAEDGVAPDMITIAKGLGAGYQPIGALLVQGRIYDAIAAGSGFFQHGHTYMGHAMAAAAANAVLD
EIEGRDLLQAVRRQGARLEALLQERLGQHPHVGDIRGRGLFRGIELVADRETKEPFDPARKLHARIKAEAFAGGLICYPM
GGTLDGVRGDHILLAPPFIISDAELDELVDKLSGALDRALAA

Sequences:

>Translated_442_residues
MPGHIFGRSTRGSLPVAAEGEGCYILDTEGRRYLDGSGGAAVSCLGHSNAAVRAALHDQLDRLAFAHTGFFTSEPAERLA
DRLAAAAPEGIERVYLVSGGSEAVEAALKLARQYFMEVGQPGRHRVIARRQSYHGNTLGALAAGGNEWRRAQFAPLLVET
SHVSPCYEYRGRAEDESLEAYGLRVADELEAEIQRLGPETVMAFVAEPVVGATAGAVPAVPGYLKRVREICDRHGVLLIL
DEVMCGMGRTGHLFACAEDGVAPDMITIAKGLGAGYQPIGALLVQGRIYDAIAAGSGFFQHGHTYMGHAMAAAAANAVLD
EIEGRDLLQAVRRQGARLEALLQERLGQHPHVGDIRGRGLFRGIELVADRETKEPFDPARKLHARIKAEAFAGGLICYPM
GGTLDGVRGDHILLAPPFIISDAELDELVDKLSGALDRALAA
>Mature_441_residues
PGHIFGRSTRGSLPVAAEGEGCYILDTEGRRYLDGSGGAAVSCLGHSNAAVRAALHDQLDRLAFAHTGFFTSEPAERLAD
RLAAAAPEGIERVYLVSGGSEAVEAALKLARQYFMEVGQPGRHRVIARRQSYHGNTLGALAAGGNEWRRAQFAPLLVETS
HVSPCYEYRGRAEDESLEAYGLRVADELEAEIQRLGPETVMAFVAEPVVGATAGAVPAVPGYLKRVREICDRHGVLLILD
EVMCGMGRTGHLFACAEDGVAPDMITIAKGLGAGYQPIGALLVQGRIYDAIAAGSGFFQHGHTYMGHAMAAAAANAVLDE
IEGRDLLQAVRRQGARLEALLQERLGQHPHVGDIRGRGLFRGIELVADRETKEPFDPARKLHARIKAEAFAGGLICYPMG
GTLDGVRGDHILLAPPFIISDAELDELVDKLSGALDRALAA

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=439, Percent_Identity=28.9293849658314, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI13994255, Length=444, Percent_Identity=28.1531531531532, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI37574042, Length=427, Percent_Identity=29.5081967213115, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI226442705, Length=427, Percent_Identity=29.5081967213115, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI226442709, Length=406, Percent_Identity=29.3103448275862, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI284507298, Length=344, Percent_Identity=29.3604651162791, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI24119277, Length=425, Percent_Identity=27.7647058823529, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI188536080, Length=389, Percent_Identity=22.879177377892, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI38679950, Length=389, Percent_Identity=22.879177377892, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI38679946, Length=389, Percent_Identity=22.879177377892, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789759, Length=429, Percent_Identity=33.7995337995338, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788044, Length=427, Percent_Identity=32.5526932084309, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789016, Length=436, Percent_Identity=34.6330275229358, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786991, Length=438, Percent_Identity=31.7351598173516, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI145693181, Length=419, Percent_Identity=32.6968973747017, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787560, Length=425, Percent_Identity=32.2352941176471, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786349, Length=347, Percent_Identity=29.1066282420749, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=448, Percent_Identity=29.0178571428571, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI25144271, Length=432, Percent_Identity=27.3148148148148, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=431, Percent_Identity=28.3062645011601, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25144274, Length=246, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17541228, Length=455, Percent_Identity=23.7362637362637, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324432, Length=423, Percent_Identity=29.3144208037825, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323470, Length=443, Percent_Identity=29.5711060948081, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324386, Length=379, Percent_Identity=27.1767810026385, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357415, Length=432, Percent_Identity=29.6296296296296, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28574759, Length=443, Percent_Identity=30.4740406320542, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI161085790, Length=439, Percent_Identity=29.6127562642369, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI21356575, Length=430, Percent_Identity=30.2325581395349, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 47141; Mature: 47010

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGHIFGRSTRGSLPVAAEGEGCYILDTEGRRYLDGSGGAAVSCLGHSNAAVRAALHDQL
CCCCCCCCCCCCCCCEEECCCCEEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHHHH
DRLAFAHTGFFTSEPAERLADRLAAAAPEGIERVYLVSGGSEAVEAALKLARQYFMEVGQ
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
PGRHRVIARRQSYHGNTLGALAAGGNEWRRAQFAPLLVETSHVSPCYEYRGRAEDESLEA
CCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCHHH
YGLRVADELEAEIQRLGPETVMAFVAEPVVGATAGAVPAVPGYLKRVREICDRHGVLLIL
HCCHHHHHHHHHHHHHCHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
DEVMCGMGRTGHLFACAEDGVAPDMITIAKGLGAGYQPIGALLVQGRIYDAIAAGSGFFQ
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCHHH
HGHTYMGHAMAAAAANAVLDEIEGRDLLQAVRRQGARLEALLQERLGQHPHVGDIRGRGL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHH
FRGIELVADRETKEPFDPARKLHARIKAEAFAGGLICYPMGGTLDGVRGDHILLAPPFII
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCEE
SDAELDELVDKLSGALDRALAA
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGHIFGRSTRGSLPVAAEGEGCYILDTEGRRYLDGSGGAAVSCLGHSNAAVRAALHDQL
CCCCCCCCCCCCCCEEECCCCEEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHHHH
DRLAFAHTGFFTSEPAERLADRLAAAAPEGIERVYLVSGGSEAVEAALKLARQYFMEVGQ
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
PGRHRVIARRQSYHGNTLGALAAGGNEWRRAQFAPLLVETSHVSPCYEYRGRAEDESLEA
CCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCHHH
YGLRVADELEAEIQRLGPETVMAFVAEPVVGATAGAVPAVPGYLKRVREICDRHGVLLIL
HCCHHHHHHHHHHHHHCHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
DEVMCGMGRTGHLFACAEDGVAPDMITIAKGLGAGYQPIGALLVQGRIYDAIAAGSGFFQ
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCHHH
HGHTYMGHAMAAAAANAVLDEIEGRDLLQAVRRQGARLEALLQERLGQHPHVGDIRGRGL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHH
FRGIELVADRETKEPFDPARKLHARIKAEAFAGGLICYPMGGTLDGVRGDHILLAPPFII
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCEE
SDAELDELVDKLSGALDRALAA
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]