Click here to switch to the map view.

The map label for this gene is ampR [H]

Identifier: 77465752

GI number: 77465752

Start: 873585

End: 874466

Strand: Reverse

Name: ampR [H]

Synonym: RSP_3748

Alternate gene names: 77465752

Gene position: 874466-873585 (Counterclockwise)

Preceding gene: 77465754

Following gene: 77465750

Centisome position: 92.73

GC content: 67.46

Gene sequence:

>882_bases
ATGCCAGATCGTCCCGACATCCCGCTGAACGCCCTGCGCACCTTCGAGGTGGCCGCTCGTCAGGGCAGCTTCACCCGCGC
AGCCGTCGAACTGCGCGTCACCCAGGCCGCCGTCAGCCACCAGATCGCGCGGCTGGAGGATATGCTGGGCGTGATCCTGT
TCCAGCGAACGCCGGGTGGCCTGATGCTGACGGATGCGGGTCAGGCGCTGTTCCCGGTGATGGAGCGCGGGCTGGACGAC
ATCGCCCGCATGCTGGACCGGGTGGCGGCGGGGCAGGCGGGCGAAGTGCTCAACATCGGCGTGGTGACGACCTTTGCCGC
GGGCTGGCTCATCCCCCGGCAGCAGGCATTCGGCCGCGCGCATCCCGGCATCACGTTGCGCCTGTCCACACACAACAACC
GGGTGGAGATCGCGCGGGAGGGGCTGGACGCGGCGATCCGCTTCGGCGACGGCCAGTGGCCGGGGCTCGAGGCGCATCAC
ATCATGGATGCCCCCATGACGCCATTCTGCGCTCCAGATCTGGGCCTGCGGGTTCCGTCAGACCTTCACCGGCACACGCT
TCTGCGCAGCTATCGGGCGGATGAATGGCCGGCCTGGTTCGCGCAGACAGGTGAAAGCTGTCCTTCTCTGACGGGGCCCA
TCCTGGACAGTTCGATCGGGCTGGCGGACCTCGCCTCCGAGAGGTTCGGGATTGCCTTGCTGCCTGCGCCGATGTTCGAA
CGCCATGTTCGGGCGGGTCGGTTGACACGCCCCTTTGCCACCACCGTGACCCTGGGCAGCTACTGGCTGACCGTGCCCAA
GGAGCGACGCATGACATCAGCATCACGGGCCTTGCTGGACTGGATGCGCGACGTGCTTCGTCAACCGCGTGCCCCTGAAT
GA

Upstream 100 bases:

>100_bases
TGCTTCATGTCCGGTCCTATGTGATCATCGGAGCCGGGATAGACCGCGCCGACTGCACGAGGCAGACGACAAAGGGTTGG
ACTGCACATAAGTAGATCTC

Downstream 100 bases:

>100_bases
CAGCGACCGCTGCGAGCTGGCCGCAGCCCGAGGACGCGGAACATGGAGGGCCGCGATTTACGAGACGCGCCCGGGTTCAC
TGAACCTGAGTGAACCCGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPDRPDIPLNALRTFEVAARQGSFTRAAVELRVTQAAVSHQIARLEDMLGVILFQRTPGGLMLTDAGQALFPVMERGLDD
IARMLDRVAAGQAGEVLNIGVVTTFAAGWLIPRQQAFGRAHPGITLRLSTHNNRVEIAREGLDAAIRFGDGQWPGLEAHH
IMDAPMTPFCAPDLGLRVPSDLHRHTLLRSYRADEWPAWFAQTGESCPSLTGPILDSSIGLADLASERFGIALLPAPMFE
RHVRAGRLTRPFATTVTLGSYWLTVPKERRMTSASRALLDWMRDVLRQPRAPE

Sequences:

>Translated_293_residues
MPDRPDIPLNALRTFEVAARQGSFTRAAVELRVTQAAVSHQIARLEDMLGVILFQRTPGGLMLTDAGQALFPVMERGLDD
IARMLDRVAAGQAGEVLNIGVVTTFAAGWLIPRQQAFGRAHPGITLRLSTHNNRVEIAREGLDAAIRFGDGQWPGLEAHH
IMDAPMTPFCAPDLGLRVPSDLHRHTLLRSYRADEWPAWFAQTGESCPSLTGPILDSSIGLADLASERFGIALLPAPMFE
RHVRAGRLTRPFATTVTLGSYWLTVPKERRMTSASRALLDWMRDVLRQPRAPE
>Mature_292_residues
PDRPDIPLNALRTFEVAARQGSFTRAAVELRVTQAAVSHQIARLEDMLGVILFQRTPGGLMLTDAGQALFPVMERGLDDI
ARMLDRVAAGQAGEVLNIGVVTTFAAGWLIPRQQAFGRAHPGITLRLSTHNNRVEIAREGLDAAIRFGDGQWPGLEAHHI
MDAPMTPFCAPDLGLRVPSDLHRHTLLRSYRADEWPAWFAQTGESCPSLTGPILDSSIGLADLASERFGIALLPAPMFER
HVRAGRLTRPFATTVTLGSYWLTVPKERRMTSASRALLDWMRDVLRQPRAPE

Specific function: This protein is a positive regulator of gene expression of cephalosporinase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=282, Percent_Identity=35.4609929078014, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=31.1188811188811, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=29.1095890410959, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=30.0699300699301, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=26.6447368421053, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=37.2549019607843, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081978, Length=152, Percent_Identity=32.8947368421053, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=265, Percent_Identity=28.3018867924528, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=24.9122807017544, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=207, Percent_Identity=28.0193236714976, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32312; Mature: 32180

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRPDIPLNALRTFEVAARQGSFTRAAVELRVTQAAVSHQIARLEDMLGVILFQRTPGG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
LMLTDAGQALFPVMERGLDDIARMLDRVAAGQAGEVLNIGVVTTFAAGWLIPRQQAFGRA
EEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHCCC
HPGITLRLSTHNNRVEIAREGLDAAIRFGDGQWPGLEAHHIMDAPMTPFCAPDLGLRVPS
CCCEEEEEECCCCCHHHHHHCCHHEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCH
DLHRHTLLRSYRADEWPAWFAQTGESCPSLTGPILDSSIGLADLASERFGIALLPAPMFE
HHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHCCCCCHHHHHHCCCCEEEECCHHHH
RHVRAGRLTRPFATTVTLGSYWLTVPKERRMTSASRALLDWMRDVLRQPRAPE
HHHHCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PDRPDIPLNALRTFEVAARQGSFTRAAVELRVTQAAVSHQIARLEDMLGVILFQRTPGG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
LMLTDAGQALFPVMERGLDDIARMLDRVAAGQAGEVLNIGVVTTFAAGWLIPRQQAFGRA
EEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHCCC
HPGITLRLSTHNNRVEIAREGLDAAIRFGDGQWPGLEAHHIMDAPMTPFCAPDLGLRVPS
CCCEEEEEECCCCCHHHHHHCCHHEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCH
DLHRHTLLRSYRADEWPAWFAQTGESCPSLTGPILDSSIGLADLASERFGIALLPAPMFE
HHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHCCCCCHHHHHHCCCCEEEECCHHHH
RHVRAGRLTRPFATTVTLGSYWLTVPKERRMTSASRALLDWMRDVLRQPRAPE
HHHHCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2788410 [H]