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The map label for this gene is azoR [H]

Identifier: 77465734

GI number: 77465734

Start: 854236

End: 854826

Strand: Reverse

Name: azoR [H]

Synonym: RSP_3728

Alternate gene names: 77465734

Gene position: 854826-854236 (Counterclockwise)

Preceding gene: 77465737

Following gene: 77465733

Centisome position: 90.65

GC content: 69.54

Gene sequence:

>591_bases
ATGACCACCATCCTCCGCATCGACAGTTCCATCAAGGGCGAGGCTGCCGTCTCGCGCCGTCTCACCCAGCGCATCCTCGA
CCGGCTTCTGGAAGCGCATCCCGATGCGACGGTCGTCTCGCGAGACCTCGCCCAGGGCATCCGTCAGATCGACGGCCCGT
GGCTCGGCTCGGTCTTCACCGCGCCCGAGCAGCGCACCGCCGATCAGCAGGAGATCGCCCGCACCGCCGGCGCCGTGATG
GCCGAGGTGAAGGAAGCCGATATTCTGGTGATCGCGCTGCCGGTCTACAACTTCGGCGCCCCCGCGCAGCTCAAGAGCTG
GGTCGACCATATCGCCCGCCGCGGCGAGAGCTTCGTCTATACCGAGACCGGCCCCGTCGGCCTGCTCACCGGCAAGCGTG
CCATCGTGGCCTTCACCTCGGACGGCACGCCGCTGGGGTCCGAGCTCGATCATGCCTCGGGCTGGCTGCGGCAGGTGCTG
GGTTTCGTGGGCATCACCGATGTGGACTTCGTGGCGGCCGACCGCATGGTCTTCGGCGCCGACGAGGCCATGGCCCGGGC
CGAGGCCGCCGTGGCAGCCCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
AGATCTGTTCGCCCTTGCACAAGTCTCGCGCGCCGGCGCGCGTTGCTTGCGGATCGGTCTAATGCCATGTGATTGGCCAC
AGCCAACAGAGGTATCCGCC

Downstream 100 bases:

>100_bases
TCCGACCGACAGACCGACGATGCAGACCGCGCCCCCTCGGGCGCGGTTTTTTCATGGGCCGCGGCAGGCAGCGCCACCGC
CCGTCCCGGCCGAGCCGCCG

Product: putative acyl carrier protein phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTTILRIDSSIKGEAAVSRRLTQRILDRLLEAHPDATVVSRDLAQGIRQIDGPWLGSVFTAPEQRTADQQEIARTAGAVM
AEVKEADILVIALPVYNFGAPAQLKSWVDHIARRGESFVYTETGPVGLLTGKRAIVAFTSDGTPLGSELDHASGWLRQVL
GFVGITDVDFVAADRMVFGADEAMARAEAAVAALAA

Sequences:

>Translated_196_residues
MTTILRIDSSIKGEAAVSRRLTQRILDRLLEAHPDATVVSRDLAQGIRQIDGPWLGSVFTAPEQRTADQQEIARTAGAVM
AEVKEADILVIALPVYNFGAPAQLKSWVDHIARRGESFVYTETGPVGLLTGKRAIVAFTSDGTPLGSELDHASGWLRQVL
GFVGITDVDFVAADRMVFGADEAMARAEAAVAALAA
>Mature_195_residues
TTILRIDSSIKGEAAVSRRLTQRILDRLLEAHPDATVVSRDLAQGIRQIDGPWLGSVFTAPEQRTADQQEIARTAGAVMA
EVKEADILVIALPVYNFGAPAQLKSWVDHIARRGESFVYTETGPVGLLTGKRAIVAFTSDGTPLGSELDHASGWLRQVLG
FVGITDVDFVAADRMVFGADEAMARAEAAVAALAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=200, Percent_Identity=36.5, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 20943; Mature: 20812

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILRIDSSIKGEAAVSRRLTQRILDRLLEAHPDATVVSRDLAQGIRQIDGPWLGSVFT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCC
APEQRTADQQEIARTAGAVMAEVKEADILVIALPVYNFGAPAQLKSWVDHIARRGESFVY
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEE
TETGPVGLLTGKRAIVAFTSDGTPLGSELDHASGWLRQVLGFVGITDVDFVAADRMVFGA
ECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECC
DEAMARAEAAVAALAA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTILRIDSSIKGEAAVSRRLTQRILDRLLEAHPDATVVSRDLAQGIRQIDGPWLGSVFT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCC
APEQRTADQQEIARTAGAVMAEVKEADILVIALPVYNFGAPAQLKSWVDHIARRGESFVY
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEE
TETGPVGLLTGKRAIVAFTSDGTPLGSELDHASGWLRQVLGFVGITDVDFVAADRMVFGA
ECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECC
DEAMARAEAAVAALAA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA