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The map label for this gene is yiaO [H]

Identifier: 77465455

GI number: 77465455

Start: 522987

End: 523970

Strand: Reverse

Name: yiaO [H]

Synonym: RSP_3453

Alternate gene names: 77465455

Gene position: 523970-522987 (Counterclockwise)

Preceding gene: 77465474

Following gene: 77465454

Centisome position: 55.56

GC content: 65.14

Gene sequence:

>984_bases
ATGAAACAGGGAAGTATCGCTGCCACTGCCGCTTTTGCCGTCTCGGTCGCCGCCACGTCGGCCTGGGCCGAAACCAAATG
GGACATGTCGATCGTCTGGCCGGAAGGCAACTTCCACACCCAGAACGCCATGGCGTTCGCGGATGCGGTGAAGGAAGCGA
CTGACGGCGAAGTGGTCATCACGGTCCATTCCGGCGGCGCGCTCGGGATCAAGGGCCCGGAAGGTATGGCCGCGGTGCGC
GACGGGCTGGTGCCGATCGCAGAGATCCTGCTCAACCAGCAGGTGGGCGAGGCTCCGGTACTCGGGATCGAGACGCTGCC
CTTCCTCGCGCCGACGATGGCCGACCTCGCGCTCCTGCACAAGTTCTACCGTCCGAAGCTCGACGAGGTCGCGGCCGGCA
TGAACCAGAAGATCCTCTACATGGTGCCGTGGCCGGGGCAGGCCGTCTTCGCGCCGAACCCGATCAACACGGTCGAGGAC
CTCAAGGGCCTCACGATCCGCGTGGTCGATTCGAACGGCAACGACTTCTTCGGCGCGCTCGGCGCCACGCCGATCCAGAT
GCCCTGGGGCGAGGTGGTGCCCTCGCTCGCGGCTGGCACCATCAAGGGCGTGACGACCTCCTCCTCGTCGGGCGTGGACG
GCGCCTTCTGGGAATTCACCAAATACATGAGCACCTTCAATTGGCAGGCTTCGTCGAACATCATGTCGGTGAACCTCGAT
GCCTGGAACGAGCTGCCGCTCGAGACCCGGCAGAAGATCGAGGAAACCGCCGCCGGCCTCGAAGGTCAGTTCTGGCTGAA
CAGCCGCGCCGAGGATGCCAAGAAGATCGCCACCCTGAAGGAGAACGGTGTCGAGGTCTCGGCTCCGTCGCCCGAACTGT
CGGCCGCGCTGCTCGAGAAGGCGCTGCCGCTCTGGGATGCCTACAAGACCCGGGTGCCGGAGGCGGCGCCGATCATCGAC
GCCTATCTGACCCTGCGCAACTGA

Upstream 100 bases:

>100_bases
GGATTGACAGATCGCGCCGGGCCGCTTCATAAATGGGATACAGTTGGTATACTGAAATCTCAAGAACAATCCAGCCAACC
AAATCCTGGGAAGGGAAAAC

Downstream 100 bases:

>100_bases
CGGGACAGGATGCGGCGGGGCGAAGGCCCCGCCCGCATTCCCCCTCTCCGGCTCCGCGACGCGGCGCCTCCTTCCCCTCG
TCGACCGTTGAGCTCCATGA

Product: TRAP-T family transporter periplasmic binding protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKQGSIAATAAFAVSVAATSAWAETKWDMSIVWPEGNFHTQNAMAFADAVKEATDGEVVITVHSGGALGIKGPEGMAAVR
DGLVPIAEILLNQQVGEAPVLGIETLPFLAPTMADLALLHKFYRPKLDEVAAGMNQKILYMVPWPGQAVFAPNPINTVED
LKGLTIRVVDSNGNDFFGALGATPIQMPWGEVVPSLAAGTIKGVTTSSSSGVDGAFWEFTKYMSTFNWQASSNIMSVNLD
AWNELPLETRQKIEETAAGLEGQFWLNSRAEDAKKIATLKENGVEVSAPSPELSAALLEKALPLWDAYKTRVPEAAPIID
AYLTLRN

Sequences:

>Translated_327_residues
MKQGSIAATAAFAVSVAATSAWAETKWDMSIVWPEGNFHTQNAMAFADAVKEATDGEVVITVHSGGALGIKGPEGMAAVR
DGLVPIAEILLNQQVGEAPVLGIETLPFLAPTMADLALLHKFYRPKLDEVAAGMNQKILYMVPWPGQAVFAPNPINTVED
LKGLTIRVVDSNGNDFFGALGATPIQMPWGEVVPSLAAGTIKGVTTSSSSGVDGAFWEFTKYMSTFNWQASSNIMSVNLD
AWNELPLETRQKIEETAAGLEGQFWLNSRAEDAKKIATLKENGVEVSAPSPELSAALLEKALPLWDAYKTRVPEAAPIID
AYLTLRN
>Mature_327_residues
MKQGSIAATAAFAVSVAATSAWAETKWDMSIVWPEGNFHTQNAMAFADAVKEATDGEVVITVHSGGALGIKGPEGMAAVR
DGLVPIAEILLNQQVGEAPVLGIETLPFLAPTMADLALLHKFYRPKLDEVAAGMNQKILYMVPWPGQAVFAPNPINTVED
LKGLTIRVVDSNGNDFFGALGATPIQMPWGEVVPSLAAGTIKGVTTSSSSGVDGAFWEFTKYMSTFNWQASSNIMSVNLD
AWNELPLETRQKIEETAAGLEGQFWLNSRAEDAKKIATLKENGVEVSAPSPELSAALLEKALPLWDAYKTRVPEAAPIID
AYLTLRN

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=325, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35003; Mature: 35003

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGSIAATAAFAVSVAATSAWAETKWDMSIVWPEGNFHTQNAMAFADAVKEATDGEVVI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEE
TVHSGGALGIKGPEGMAAVRDGLVPIAEILLNQQVGEAPVLGIETLPFLAPTMADLALLH
EEECCCEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHH
KFYRPKLDEVAAGMNQKILYMVPWPGQAVFAPNPINTVEDLKGLTIRVVDSNGNDFFGAL
HHHCCCHHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHCCCCEEEEEECCCCEEEECC
GATPIQMPWGEVVPSLAAGTIKGVTTSSSSGVDGAFWEFTKYMSTFNWQASSNIMSVNLD
CCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECC
AWNELPLETRQKIEETAAGLEGQFWLNSRAEDAKKIATLKENGVEVSAPSPELSAALLEK
CCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
ALPLWDAYKTRVPEAAPIIDAYLTLRN
HCCHHHHHHHCCCCCHHHEEHEEEECC
>Mature Secondary Structure
MKQGSIAATAAFAVSVAATSAWAETKWDMSIVWPEGNFHTQNAMAFADAVKEATDGEVVI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEE
TVHSGGALGIKGPEGMAAVRDGLVPIAEILLNQQVGEAPVLGIETLPFLAPTMADLALLH
EEECCCEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHH
KFYRPKLDEVAAGMNQKILYMVPWPGQAVFAPNPINTVEDLKGLTIRVVDSNGNDFFGAL
HHHCCCHHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHCCCCEEEEEECCCCEEEECC
GATPIQMPWGEVVPSLAAGTIKGVTTSSSSGVDGAFWEFTKYMSTFNWQASSNIMSVNLD
CCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECC
AWNELPLETRQKIEETAAGLEGQFWLNSRAEDAKKIATLKENGVEVSAPSPELSAALLEK
CCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
ALPLWDAYKTRVPEAAPIIDAYLTLRN
HCCHHHHHHHCCCCCHHHEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]