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The map label for this gene is potF [C]

Identifier: 77465398

GI number: 77465398

Start: 461068

End: 462111

Strand: Reverse

Name: potF [C]

Synonym: RSP_3397

Alternate gene names: 77465398

Gene position: 462111-461068 (Counterclockwise)

Preceding gene: 77465399

Following gene: 77465397

Centisome position: 49.0

GC content: 67.62

Gene sequence:

>1044_bases
ATGACGAACAGAAGCCTTGCGGCGAGCGCGGCGGTTCTGGCGCTCACGGCCGGAGCGGCGCAGGCCGAGACGGTGGTGCT
GTCGGCCTATTCGGGCATCTTCGAGGAGAATTACCGCGCGGCGGTGGTCGAGCCCTTCATGGAGGCGCATCCCGACATCG
ACGTGGAATTCTACGGCATGCCCAACTCCGCCCAGATGCTCGGCACCCTGCGGGCGCAGAAGTCCGCGCCCCAGATCGAC
GTGATGATCCTTGACGTCTCGGTCGCCAAGGCCGCCACCGACGAAGGGCTGTTCGCTCCCCTCGACGAGAGCGTGACCGC
CCATGTGGCCGATCTCTATCCGCAGGCCCGCGTCGAGGGCGTGAATGCGGTGGGCGTCACCTTCGACAACCTCGTGATGC
TCTACAACACCGACAAGGTGACCGAGGCCCCGACCTCGTGGGAGGCGCTCTGGGATCCGCAGTGGAAGGGGCAGGTCTCG
ATCCCCGCGGTGCCCGACATCCAGGGCACCACCCTCACCATCATCGCGAACCGCCTCGCGGGCGGCGAGGGCTACATGGA
CGGAATGGAGCCGGGGATCGGCAAGCTCATGGAGCTCGCGCCGAACGTCCAGACCTGGGAGCCGCGCCCCGACGTCTATC
AGCCGATCTCGAGCGGGACCGCCTCGATCGGCATCGGCTGGAACGCGCGGGCGCAGGTCTATGCCGACAAGTCCGACGGC
AAGCTCGGCGTCGTGCTGCCCGAAGAGGGAAGCGGCTTCCAGATCAACGTGATCGGCCTCGTGCAGGATGCCCCCGCGGG
CGATGCGGCCAGGACCTTCCTCGACTATGCCCTGAGCCCCGAGGTGCAGGCGCGCTTCACCGAGACCATGTTCTACGCGC
CCACCAACCAGAAGGCCGCGGCGCTCGTCTCCGAAGCGGCGCTCGAGCGGACGGCGGCGGGCTCCATGGACGAGATGATC
CCGATCAACTGGCTCGAGGTTGCCACGATCCGCGATGCCGTCACCGAACAGTGGCGCCGCCGCATCATCCCGCTGAGCCG
CTGA

Upstream 100 bases:

>100_bases
GGGGCCACGAAGCTCGTCGAGGCCTGAGGGGCCCCCGGCTCCGGCCGTGCCCCGCGCGGCCGGGACCCATTCACGAGAAG
CAACAGGGAAAGGCAACGTC

Downstream 100 bases:

>100_bases
CATGATGGTCGAGCGCGCGATGACCCCGGAGGCCGCCCTCGGGACCCCGGAGGGTCCCGATCACCTGGTCCTGAGCGAGG
TCGGCATGACCTTCGGGACG

Product: ABC opine/polyamine transporter, periplasmic binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTNRSLAASAAVLALTAGAAQAETVVLSAYSGIFEENYRAAVVEPFMEAHPDIDVEFYGMPNSAQMLGTLRAQKSAPQID
VMILDVSVAKAATDEGLFAPLDESVTAHVADLYPQARVEGVNAVGVTFDNLVMLYNTDKVTEAPTSWEALWDPQWKGQVS
IPAVPDIQGTTLTIIANRLAGGEGYMDGMEPGIGKLMELAPNVQTWEPRPDVYQPISSGTASIGIGWNARAQVYADKSDG
KLGVVLPEEGSGFQINVIGLVQDAPAGDAARTFLDYALSPEVQARFTETMFYAPTNQKAAALVSEAALERTAAGSMDEMI
PINWLEVATIRDAVTEQWRRRIIPLSR

Sequences:

>Translated_347_residues
MTNRSLAASAAVLALTAGAAQAETVVLSAYSGIFEENYRAAVVEPFMEAHPDIDVEFYGMPNSAQMLGTLRAQKSAPQID
VMILDVSVAKAATDEGLFAPLDESVTAHVADLYPQARVEGVNAVGVTFDNLVMLYNTDKVTEAPTSWEALWDPQWKGQVS
IPAVPDIQGTTLTIIANRLAGGEGYMDGMEPGIGKLMELAPNVQTWEPRPDVYQPISSGTASIGIGWNARAQVYADKSDG
KLGVVLPEEGSGFQINVIGLVQDAPAGDAARTFLDYALSPEVQARFTETMFYAPTNQKAAALVSEAALERTAAGSMDEMI
PINWLEVATIRDAVTEQWRRRIIPLSR
>Mature_346_residues
TNRSLAASAAVLALTAGAAQAETVVLSAYSGIFEENYRAAVVEPFMEAHPDIDVEFYGMPNSAQMLGTLRAQKSAPQIDV
MILDVSVAKAATDEGLFAPLDESVTAHVADLYPQARVEGVNAVGVTFDNLVMLYNTDKVTEAPTSWEALWDPQWKGQVSI
PAVPDIQGTTLTIIANRLAGGEGYMDGMEPGIGKLMELAPNVQTWEPRPDVYQPISSGTASIGIGWNARAQVYADKSDGK
LGVVLPEEGSGFQINVIGLVQDAPAGDAARTFLDYALSPEVQARFTETMFYAPTNQKAAALVSEAALERTAAGSMDEMIP
INWLEVATIRDAVTEQWRRRIIPLSR

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787078, Length=319, Percent_Identity=23.8244514106583, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37279; Mature: 37148

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRSLAASAAVLALTAGAAQAETVVLSAYSGIFEENYRAAVVEPFMEAHPDIDVEFYGM
CCCCHHHHHHEEEEEECCCCCHHEEEEEHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEC
PNSAQMLGTLRAQKSAPQIDVMILDVSVAKAATDEGLFAPLDESVTAHVADLYPQARVEG
CCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHCC
VNAVGVTFDNLVMLYNTDKVTEAPTSWEALWDPQWKGQVSIPAVPDIQGTTLTIIANRLA
CEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCEEEEEEEHHC
GGEGYMDGMEPGIGKLMELAPNVQTWEPRPDVYQPISSGTASIGIGWNARAQVYADKSDG
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEEECCCC
KLGVVLPEEGSGFQINVIGLVQDAPAGDAARTFLDYALSPEVQARFTETMFYAPTNQKAA
EEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHEEEEEEEECCCCCHHH
ALVSEAALERTAAGSMDEMIPINWLEVATIRDAVTEQWRRRIIPLSR
HHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNRSLAASAAVLALTAGAAQAETVVLSAYSGIFEENYRAAVVEPFMEAHPDIDVEFYGM
CCCHHHHHHEEEEEECCCCCHHEEEEEHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEC
PNSAQMLGTLRAQKSAPQIDVMILDVSVAKAATDEGLFAPLDESVTAHVADLYPQARVEG
CCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHCC
VNAVGVTFDNLVMLYNTDKVTEAPTSWEALWDPQWKGQVSIPAVPDIQGTTLTIIANRLA
CEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCEEEEEEEHHC
GGEGYMDGMEPGIGKLMELAPNVQTWEPRPDVYQPISSGTASIGIGWNARAQVYADKSDG
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEEECCCC
KLGVVLPEEGSGFQINVIGLVQDAPAGDAARTFLDYALSPEVQARFTETMFYAPTNQKAA
EEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHEEEEEEEECCCCCHHH
ALVSEAALERTAAGSMDEMIPINWLEVATIRDAVTEQWRRRIIPLSR
HHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]