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The map label for this gene is macA [H]

Identifier: 77465377

GI number: 77465377

Start: 439666

End: 440943

Strand: Reverse

Name: macA [H]

Synonym: RSP_3375

Alternate gene names: 77465377

Gene position: 440943-439666 (Counterclockwise)

Preceding gene: 77465378

Following gene: 77465376

Centisome position: 46.76

GC content: 73.55

Gene sequence:

>1278_bases
ATGGCCGCCGGAGAAGGACCCATGACCGACAGGAGCGACGATCTCGCCCGCATCCTCGACCGACGCCGCCGCGGGCGGCG
CTGGCTCTGGCCCCTGCTGGCGCTGGGGCTGCTTGCGGGCGGGGGCCTCCTCTGGGCCTCGCGGCAGGGGGCGGAGCCCG
CGCTTGCCTATGTGACCGAGCCGGTGGAGCGGGGCGGGCTTGTCGTGACGGTGATGGCCACGGGCACGGTGCAGCCCACG
ACGCAGGTCGAGGTTTCCTCGGAACTGTCGGGCACGCTCGCCGCGGTGGAGGTGGATTACAACGACGAGGTCGCGGTGGG
CCAGCCGCTCGCCCGGCTGGACGACACGCGGCTGAAGGCGCAGGTCGCCAATGCCGAGGCCTCGCTCGAGGCGGCGGAGG
GGCGGCTCGAACAGGCCCGCGCCACCGCGCTCGAGGCGGCCGAGAACTACCGCTCGCGCTCGGCGCTCGACCAGCGGGGC
GTGGCCGCCCATCTCGATCTCGTGGCGACCGAGGCCGCCCACAAGCGCGCGGAATCCGAGGTAAAGATCGCCGAGGCCGA
CCGCACGCTCGCGGCTGCCAATCTCGAGGTGCAGCGGGTGGATCTGGCCAAGGCCGTGATCCGCTCGCCGATCCGCGGCA
TCGTGCTCGACCGGGCAGCGGAGGCGGGGCAGATCGTGGCCTCCTCGCTGAACACGCCGGTGCTGTTCACGCTGGCCGAG
GATCTGACCCGGATGGAGCTGCGCGTCGACATCGACGAGGCCGACATCGGCCGGGTGCGGGTGGGCAACCGCGCCTCCTT
CAAGGTGGACGCCTATCCGGGGCGGAGCTTCAAGGCCGAGATCGTGCAGGTGCGCTACGCGCCCGAGACGACCGACGGCG
TGGTGACCTACAAGGCGGTGCTGTCGGTGGACAATGGCGAGCGGCTGCTGCGGCCCGGTATGACCGCGACCGCGACGATC
ACGGTGGCCGAGGTGGAGGGCGCGCTCCTCGTGCCGAATGCCGCGCTGCGCTTCGCCCCGCCGGCGGTGGCCCAGCCCGG
CAGTTCCGGCGGCAACCGCGGGGGCGGGCTGCTCGGGCTCATCATGCCCCGCGCCCCCGGGCCGATGAGCGAGGCGGACG
CCACGTCGGGCCCCACGGTCTGGGTCCTGCGCGACGGTGAGCCCGACCGGGTGGCGGTCGAGACCGGCGAGACCGACGGG
CGGCGGACCGAGATCCGCGGCGGCGCGCTCGAGGAGGGGGCTCCGGTCATCACCGATCAGCGCGAGGCCGGAGCTTGA

Upstream 100 bases:

>100_bases
GCGGCGGATCTGTTCGCTCTGCGCAGGCGGCTGAGCCGGGTTCGCATGATCTGGCGCAAGTCCTTGGCGGGGCGGCTCGC
CTAAGCAGGGCACGACAGGG

Downstream 100 bases:

>100_bases
CCGAGGGGCCGCTCATCGCGCTTCAGGGGATCGGCCGGTCCTATGGCGAGGGCGAGGCCGAGGTGCGCGCGCTCGACGGG
GTGGATCTCACCATCCGGCG

Product: HlyD family membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAAGEGPMTDRSDDLARILDRRRRGRRWLWPLLALGLLAGGGLLWASRQGAEPALAYVTEPVERGGLVVTVMATGTVQPT
TQVEVSSELSGTLAAVEVDYNDEVAVGQPLARLDDTRLKAQVANAEASLEAAEGRLEQARATALEAAENYRSRSALDQRG
VAAHLDLVATEAAHKRAESEVKIAEADRTLAAANLEVQRVDLAKAVIRSPIRGIVLDRAAEAGQIVASSLNTPVLFTLAE
DLTRMELRVDIDEADIGRVRVGNRASFKVDAYPGRSFKAEIVQVRYAPETTDGVVTYKAVLSVDNGERLLRPGMTATATI
TVAEVEGALLVPNAALRFAPPAVAQPGSSGGNRGGGLLGLIMPRAPGPMSEADATSGPTVWVLRDGEPDRVAVETGETDG
RRTEIRGGALEEGAPVITDQREAGA

Sequences:

>Translated_425_residues
MAAGEGPMTDRSDDLARILDRRRRGRRWLWPLLALGLLAGGGLLWASRQGAEPALAYVTEPVERGGLVVTVMATGTVQPT
TQVEVSSELSGTLAAVEVDYNDEVAVGQPLARLDDTRLKAQVANAEASLEAAEGRLEQARATALEAAENYRSRSALDQRG
VAAHLDLVATEAAHKRAESEVKIAEADRTLAAANLEVQRVDLAKAVIRSPIRGIVLDRAAEAGQIVASSLNTPVLFTLAE
DLTRMELRVDIDEADIGRVRVGNRASFKVDAYPGRSFKAEIVQVRYAPETTDGVVTYKAVLSVDNGERLLRPGMTATATI
TVAEVEGALLVPNAALRFAPPAVAQPGSSGGNRGGGLLGLIMPRAPGPMSEADATSGPTVWVLRDGEPDRVAVETGETDG
RRTEIRGGALEEGAPVITDQREAGA
>Mature_424_residues
AAGEGPMTDRSDDLARILDRRRRGRRWLWPLLALGLLAGGGLLWASRQGAEPALAYVTEPVERGGLVVTVMATGTVQPTT
QVEVSSELSGTLAAVEVDYNDEVAVGQPLARLDDTRLKAQVANAEASLEAAEGRLEQARATALEAAENYRSRSALDQRGV
AAHLDLVATEAAHKRAESEVKIAEADRTLAAANLEVQRVDLAKAVIRSPIRGIVLDRAAEAGQIVASSLNTPVLFTLAED
LTRMELRVDIDEADIGRVRVGNRASFKVDAYPGRSFKAEIVQVRYAPETTDGVVTYKAVLSVDNGERLLRPGMTATATIT
VAEVEGALLVPNAALRFAPPAVAQPGSSGGNRGGGLLGLIMPRAPGPMSEADATSGPTVWVLRDGEPDRVAVETGETDGR
RTEIRGGALEEGAPVITDQREAGA

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=318, Percent_Identity=29.559748427673, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44885; Mature: 44754

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGEGPMTDRSDDLARILDRRRRGRRWLWPLLALGLLAGGGLLWASRQGAEPALAYVTE
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHC
PVERGGLVVTVMATGTVQPTTQVEVSSELSGTLAAVEVDYNDEVAVGQPLARLDDTRLKA
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHCCHHHHE
QVANAEASLEAAEGRLEQARATALEAAENYRSRSALDQRGVAAHLDLVATEAAHKRAESE
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCC
VKIAEADRTLAAANLEVQRVDLAKAVIRSPIRGIVLDRAAEAGQIVASSLNTPVLFTLAE
EEEECCCCEEEECCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCCEEEEEHH
DLTRMELRVDIDEADIGRVRVGNRASFKVDAYPGRSFKAEIVQVRYAPETTDGVVTYKAV
HHEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEE
LSVDNGERLLRPGMTATATITVAEVEGALLVPNAALRFAPPAVAQPGSSGGNRGGGLLGL
EEECCCCHHHCCCCCEEEEEEEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEE
IMPRAPGPMSEADATSGPTVWVLRDGEPDRVAVETGETDGRRTEIRGGALEEGAPVITDQ
EECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCCCCEECC
REAGA
HHCCC
>Mature Secondary Structure 
AAGEGPMTDRSDDLARILDRRRRGRRWLWPLLALGLLAGGGLLWASRQGAEPALAYVTE
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHC
PVERGGLVVTVMATGTVQPTTQVEVSSELSGTLAAVEVDYNDEVAVGQPLARLDDTRLKA
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHCCHHHHE
QVANAEASLEAAEGRLEQARATALEAAENYRSRSALDQRGVAAHLDLVATEAAHKRAESE
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCC
VKIAEADRTLAAANLEVQRVDLAKAVIRSPIRGIVLDRAAEAGQIVASSLNTPVLFTLAE
EEEECCCCEEEECCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCCEEEEEHH
DLTRMELRVDIDEADIGRVRVGNRASFKVDAYPGRSFKAEIVQVRYAPETTDGVVTYKAV
HHEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEE
LSVDNGERLLRPGMTATATITVAEVEGALLVPNAALRFAPPAVAQPGSSGGNRGGGLLGL
EEECCCCHHHCCCCCEEEEEEEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEE
IMPRAPGPMSEADATSGPTVWVLRDGEPDRVAVETGETDGRRTEIRGGALEEGAPVITDQ
EECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCCCCEECC
REAGA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]