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The map label for this gene is yafC [H]

Identifier: 77465303

GI number: 77465303

Start: 357349

End: 358290

Strand: Direct

Name: yafC [H]

Synonym: RSP_3298

Alternate gene names: 77465303

Gene position: 357349-358290 (Clockwise)

Preceding gene: 77465291

Following gene: 77465304

Centisome position: 37.89

GC content: 65.39

Gene sequence:

>942_bases
ATGGGCAATTTCACATGGGACGACCTGCAGTTCTTCCTCGCGGTGGCGCGCTCGGGGCAACTGTCGCGCGCGGCGCGCCA
GCTCCGCACGAGCCATGTCACCGTCTCGCGCCGCATCGACCGGCTGGAAGCCGCGCTGCGGATGCGGCTCTTCGAGCGCA
ACCCGCGCGGCTACGAGCTGACGCCGGTGGGTCGGCGGCTGATCGAGACGGCGGAGCGGATGGAGGCCGAGACCGAGCGG
ATGCAGGTCGAACTGCTGGGGGACGGGCCGGGCCAGCGCGGCGTCCTGCGCATGGCCGTGCCCGAGGGGTTCGGCAGCTG
GTTCAGCCGGGTGCTGCTCGGCCCCTTCACCGAGCGGTTTCCCAACATCGCGCTCGAGCTCATCACGCTGCCGCAGATCA
TGTCGCTCTCGCGGCGCGAGGCGGATCTGACGGTGACGCTCGACCCTCCGAAGGCGAGTACTTACCACTCTGAGAGGATC
ATGGATTACACGCTGCATATCTACGCCGCCAAATCCTATCTTGCGCAGCGGCCCGAGATCCAGACCCGGGACGATCTGCT
GAAACATCCTTTCATCGGATACATCGAAGAGATGATCTTTGCCCCGGGTCTGGATTATCTGGGCGAGGTCCATCCCGGCA
TCCGCGCCAATTTCAAGAGCTCGTCGATCTTCAACCAGCTCGCCGCGACGCAGAACGGGCTCGGGCTCTGCGTGCTGCCC
TTCTACATCGGCCGGAGGGTTCCGGATCTGGAAATGATCCTGCCGCAGGAAGTGGTTCTGCGCCGAACCTACTGGCTGAC
CTGCCACCGCGACGTTCGGCCGCTGCCCCGCGAACGCTCGGTCATGGCCTTCCTTCTCGAGGCCGGGCGCACCCATGGGC
GCAGCATTCTCAACTCTGGCGCGACCCGAAAGGGCGATGGATCTACCACCTTCGTCCAGTAG

Upstream 100 bases:

>100_bases
CTGGAAGTAGGATCAGACGTTTCGGGGCTGGACTCAAGCAATTTGTCGCAGCTATTTCGGAACAAGATCTGTTCATTTTC
GGACAGACAAAGGGTGACGC

Downstream 100 bases:

>100_bases
CGACCCTATTGGCGTGGATCGACGGGACAAGAGCATGCTGTATTCTATCTGAAATCTGTTGCCATGCCGGGATGCGGCCA
TGAGCTCGAGTCATTTGAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MGNFTWDDLQFFLAVARSGQLSRAARQLRTSHVTVSRRIDRLEAALRMRLFERNPRGYELTPVGRRLIETAERMEAETER
MQVELLGDGPGQRGVLRMAVPEGFGSWFSRVLLGPFTERFPNIALELITLPQIMSLSRREADLTVTLDPPKASTYHSERI
MDYTLHIYAAKSYLAQRPEIQTRDDLLKHPFIGYIEEMIFAPGLDYLGEVHPGIRANFKSSSIFNQLAATQNGLGLCVLP
FYIGRRVPDLEMILPQEVVLRRTYWLTCHRDVRPLPRERSVMAFLLEAGRTHGRSILNSGATRKGDGSTTFVQ

Sequences:

>Translated_313_residues
MGNFTWDDLQFFLAVARSGQLSRAARQLRTSHVTVSRRIDRLEAALRMRLFERNPRGYELTPVGRRLIETAERMEAETER
MQVELLGDGPGQRGVLRMAVPEGFGSWFSRVLLGPFTERFPNIALELITLPQIMSLSRREADLTVTLDPPKASTYHSERI
MDYTLHIYAAKSYLAQRPEIQTRDDLLKHPFIGYIEEMIFAPGLDYLGEVHPGIRANFKSSSIFNQLAATQNGLGLCVLP
FYIGRRVPDLEMILPQEVVLRRTYWLTCHRDVRPLPRERSVMAFLLEAGRTHGRSILNSGATRKGDGSTTFVQ
>Mature_312_residues
GNFTWDDLQFFLAVARSGQLSRAARQLRTSHVTVSRRIDRLEAALRMRLFERNPRGYELTPVGRRLIETAERMEAETERM
QVELLGDGPGQRGVLRMAVPEGFGSWFSRVLLGPFTERFPNIALELITLPQIMSLSRREADLTVTLDPPKASTYHSERIM
DYTLHIYAAKSYLAQRPEIQTRDDLLKHPFIGYIEEMIFAPGLDYLGEVHPGIRANFKSSSIFNQLAATQNGLGLCVLPF
YIGRRVPDLEMILPQEVVLRRTYWLTCHRDVRPLPRERSVMAFLLEAGRTHGRSILNSGATRKGDGSTTFVQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=26.7558528428094, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI157672245, Length=144, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789639, Length=303, Percent_Identity=23.4323432343234, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=21.6949152542373, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35678; Mature: 35546

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNFTWDDLQFFLAVARSGQLSRAARQLRTSHVTVSRRIDRLEAALRMRLFERNPRGYEL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TPVGRRLIETAERMEAETERMQVELLGDGPGQRGVLRMAVPEGFGSWFSRVLLGPFTERF
CHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
PNIALELITLPQIMSLSRREADLTVTLDPPKASTYHSERIMDYTLHIYAAKSYLAQRPEI
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
QTRDDLLKHPFIGYIEEMIFAPGLDYLGEVHPGIRANFKSSSIFNQLAATQNGLGLCVLP
CCHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHH
FYIGRRVPDLEMILPQEVVLRRTYWLTCHRDVRPLPRERSVMAFLLEAGRTHGRSILNSG
HHHCCCCCCHHHHCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
ATRKGDGSTTFVQ
CCCCCCCCCCCCC
>Mature Secondary Structure 
GNFTWDDLQFFLAVARSGQLSRAARQLRTSHVTVSRRIDRLEAALRMRLFERNPRGYEL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TPVGRRLIETAERMEAETERMQVELLGDGPGQRGVLRMAVPEGFGSWFSRVLLGPFTERF
CHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
PNIALELITLPQIMSLSRREADLTVTLDPPKASTYHSERIMDYTLHIYAAKSYLAQRPEI
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
QTRDDLLKHPFIGYIEEMIFAPGLDYLGEVHPGIRANFKSSSIFNQLAATQNGLGLCVLP
CCHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHH
FYIGRRVPDLEMILPQEVVLRRTYWLTCHRDVRPLPRERSVMAFLLEAGRTHGRSILNSG
HHHCCCCCCHHHHCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
ATRKGDGSTTFVQ
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]