Click here to switch to the map view.

The map label for this gene is rnfF [H]

Identifier: 77465202

GI number: 77465202

Start: 242304

End: 243878

Strand: Reverse

Name: rnfF [H]

Synonym: RSP_3188

Alternate gene names: 77465202

Gene position: 243878-242304 (Counterclockwise)

Preceding gene: 77465203

Following gene: 77465201

Centisome position: 25.86

GC content: 75.75

Gene sequence:

>1575_bases
ATGGCGGGCGCCGGCACCCCGGCCGATCCGCGCCTGCTGCGCGAACGGCTGAGGGTGGTCGCCGCACCTGCCGGGCGCTT
GCGGCTCACGGCCCCGCGCGCCGCGGGCTGCGCCGGCTGCGGCGCGCGCGCGGCCTGCGGCGGGGCGGCGGCCTCCGGCC
CCGCCCGGCTGCTCGAGATCGCCTGCCCGCCGGACCTCGCGCCCCGGCCCGGCGATGAGGTCGAAGTCGCTCTGTCCGGC
GGGCGCTTCCTCGCGGCGGTGGGGCTGGCCTATCTGCTGCCCACGCTGGCGGTCGTGATCGCGGCCGGCGCGGGGCTGGC
GCTGCAGCTGTCCGATCTGGGCGTGGCGCTCCTGCTGCCTGCCCGCCCTTGCGCTCTCGCTCATCCCCCTTGCCCTCGCC
GGCCGGGGCGAGGGGCTGACAGAGGCGCTCCGCGTCGAGGCCCTCCACCCCGCCGGGCCTTCCGAACCCGACCCATGCGG
ACCGTGACCCTCCCGCGCCGGGCCTTCCTTGCTACCCTCGCCGCGCTCGCGCTCGGCGGGGTGGCGGCCCTGCGCCTCGA
GCCCGCCTCGGACGTTCGCGAGACCTTCTATCTCTTCGGCACGCTGGTAGAGATCGAGACCCACGGCGTGCCCGAACGCA
TGGCCCGCGCCGCCATCGCCCGCGCGGGCCAGAGCCTTGAGGCCGCGCATCGCGACTGGCACGGCTGGCGGCCGGGCGAA
CTCGGGACCTTGAACACGGCCATCGCGGCGGGGCGCCCGCAGCGCGTGGATGCGACCCTCGCGGCCCTCCTGCGCAAGGG
GCGCGCCCTCTCCTGTGCGAGCGGCGGGCTGTTCGATCCGGCCATCGGCAGGCTGGTCGGCGCCTGGGGCTTTCACGCCG
ACACATTGCCCGAGGGCGATCCGCCATCCGCCGCCGCCATCGCGGCCCTCGTCGCGCGCCACCCGCGCATGACCGACCTG
CGCTTCGACGGCACCGAGGTGCGCTCGCTCAATCCCGCGGTTCAACTCGATCTCGGGGCCTATGCCAAGGGGGCGGCGCT
CGAGATGGCGGCGGCCGAGCTTCGGGCGGCAGGCGTCGAGGATGCGGTGCTGAACGCCGGCGGCGGTGTGCAGGTCATGG
GCCGCCACGGCGCGCGGCCCTGGCGGGTGGCGATCCGCGATCCGTTCCAGTGGGGCGTGGTGGCGGGCGTTTCGCTCCGC
CCCGGCGAGGCCCTGCATACGTCGGGCAATTACGAGCGCTATTTCGACCGCGGCGGTGTGCGCTTCTCGCACATCCTCGA
TCCGCGGACGGGCCGTCCGATGCAGGGCATCGTCTCGGTCTCGGTGCTCGACACCGACGGCGCGCGGGCCGATGCCGCGG
CCACCGCGCTCTGCATCGCGGGCCGCGAGGCCTGGCCGCGGGTCGCGGCGGCGATGGGCGTGCGCGCCGTCCTGATGATC
GCGGACGATGGCGCGGTCTTCGCCACGCCCGAGATGATGGCGCGGCTCGAACCGGCGCAGGGCGGCTACCCCGCCCCGGT
GCAGATCGTATCGCTGCCGGAGGATGTCACACCGCCCGCCTGTCCCGAGGACTGA

Upstream 100 bases:

>100_bases
AAGTCCGAGGTCTGCACCGAATGCGAGGGCGCCTTCGACATGCCGCAGTGCATGAGCGTCTGCACCTCCGACAGCATCGC
CCCGCTGGAGGCCTGAGCCG

Downstream 100 bases:

>100_bases
CGGACCCCGCACCTCCGCTTCCGGCGGAAGGTATCGCGAGGCCCGGGCGACCGGCAATCGCACGACATGGGTCGCGGAGG
CCGCCCAATCAAGCGCTTCG

Product: ApbE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAGAGTPADPRLLRERLRVVAAPAGRLRLTAPRAAGCAGCGARAACGGAAASGPARLLEIACPPDLAPRPGDEVEVALSG
GRFLAAVGLAYLLPTLAVVIAAGAGLALQLSDLGVALLLPARPCALAHPPCPRRPGRGADRGAPRRGPPPRRAFRTRPMR
TVTLPRRAFLATLAALALGGVAALRLEPASDVRETFYLFGTLVEIETHGVPERMARAAIARAGQSLEAAHRDWHGWRPGE
LGTLNTAIAAGRPQRVDATLAALLRKGRALSCASGGLFDPAIGRLVGAWGFHADTLPEGDPPSAAAIAALVARHPRMTDL
RFDGTEVRSLNPAVQLDLGAYAKGAALEMAAAELRAAGVEDAVLNAGGGVQVMGRHGARPWRVAIRDPFQWGVVAGVSLR
PGEALHTSGNYERYFDRGGVRFSHILDPRTGRPMQGIVSVSVLDTDGARADAAATALCIAGREAWPRVAAAMGVRAVLMI
ADDGAVFATPEMMARLEPAQGGYPAPVQIVSLPEDVTPPACPED

Sequences:

>Translated_524_residues
MAGAGTPADPRLLRERLRVVAAPAGRLRLTAPRAAGCAGCGARAACGGAAASGPARLLEIACPPDLAPRPGDEVEVALSG
GRFLAAVGLAYLLPTLAVVIAAGAGLALQLSDLGVALLLPARPCALAHPPCPRRPGRGADRGAPRRGPPPRRAFRTRPMR
TVTLPRRAFLATLAALALGGVAALRLEPASDVRETFYLFGTLVEIETHGVPERMARAAIARAGQSLEAAHRDWHGWRPGE
LGTLNTAIAAGRPQRVDATLAALLRKGRALSCASGGLFDPAIGRLVGAWGFHADTLPEGDPPSAAAIAALVARHPRMTDL
RFDGTEVRSLNPAVQLDLGAYAKGAALEMAAAELRAAGVEDAVLNAGGGVQVMGRHGARPWRVAIRDPFQWGVVAGVSLR
PGEALHTSGNYERYFDRGGVRFSHILDPRTGRPMQGIVSVSVLDTDGARADAAATALCIAGREAWPRVAAAMGVRAVLMI
ADDGAVFATPEMMARLEPAQGGYPAPVQIVSLPEDVTPPACPED
>Mature_523_residues
AGAGTPADPRLLRERLRVVAAPAGRLRLTAPRAAGCAGCGARAACGGAAASGPARLLEIACPPDLAPRPGDEVEVALSGG
RFLAAVGLAYLLPTLAVVIAAGAGLALQLSDLGVALLLPARPCALAHPPCPRRPGRGADRGAPRRGPPPRRAFRTRPMRT
VTLPRRAFLATLAALALGGVAALRLEPASDVRETFYLFGTLVEIETHGVPERMARAAIARAGQSLEAAHRDWHGWRPGEL
GTLNTAIAAGRPQRVDATLAALLRKGRALSCASGGLFDPAIGRLVGAWGFHADTLPEGDPPSAAAIAALVARHPRMTDLR
FDGTEVRSLNPAVQLDLGAYAKGAALEMAAAELRAAGVEDAVLNAGGGVQVMGRHGARPWRVAIRDPFQWGVVAGVSLRP
GEALHTSGNYERYFDRGGVRFSHILDPRTGRPMQGIVSVSVLDTDGARADAAATALCIAGREAWPRVAAAMGVRAVLMIA
DDGAVFATPEMMARLEPAQGGYPAPVQIVSLPEDVTPPACPED

Specific function: Required for nitrogen fixation [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseC family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=247, Percent_Identity=31.1740890688259, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007359
- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE; PF04246 RseC_MucC [H]

EC number: NA

Molecular weight: Translated: 54305; Mature: 54174

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAGTPADPRLLRERLRVVAAPAGRLRLTAPRAAGCAGCGARAACGGAAASGPARLLEI
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCCEEEE
ACPPDLAPRPGDEVEVALSGGRFLAAVGLAYLLPTLAVVIAAGAGLALQLSDLGVALLLP
ECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEC
ARPCALAHPPCPRRPGRGADRGAPRRGPPPRRAFRTRPMRTVTLPRRAFLATLAALALGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHCC
VAALRLEPASDVRETFYLFGTLVEIETHGVPERMARAAIARAGQSLEAAHRDWHGWRPGE
EEEEEECCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
LGTLNTAIAAGRPQRVDATLAALLRKGRALSCASGGLFDPAIGRLVGAWGFHADTLPEGD
CCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCC
PPSAAAIAALVARHPRMTDLRFDGTEVRSLNPAVQLDLGAYAKGAALEMAAAELRAAGVE
CCHHHHHHHHHHCCCCCCEEEECCCHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
DAVLNAGGGVQVMGRHGARPWRVAIRDPFQWGVVAGVSLRPGEALHTSGNYERYFDRGGV
HHHCCCCCCEEEEECCCCCCEEEEEECCCCCCEEECCEECCCCCEECCCCHHHHHHCCCC
RFSHILDPRTGRPMQGIVSVSVLDTDGARADAAATALCIAGREAWPRVAAAMGVRAVLMI
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEE
ADDGAVFATPEMMARLEPAQGGYPAPVQIVSLPEDVTPPACPED
ECCCCEEECHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AGAGTPADPRLLRERLRVVAAPAGRLRLTAPRAAGCAGCGARAACGGAAASGPARLLEI
CCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCCEEEE
ACPPDLAPRPGDEVEVALSGGRFLAAVGLAYLLPTLAVVIAAGAGLALQLSDLGVALLLP
ECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEC
ARPCALAHPPCPRRPGRGADRGAPRRGPPPRRAFRTRPMRTVTLPRRAFLATLAALALGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHCC
VAALRLEPASDVRETFYLFGTLVEIETHGVPERMARAAIARAGQSLEAAHRDWHGWRPGE
EEEEEECCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
LGTLNTAIAAGRPQRVDATLAALLRKGRALSCASGGLFDPAIGRLVGAWGFHADTLPEGD
CCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCC
PPSAAAIAALVARHPRMTDLRFDGTEVRSLNPAVQLDLGAYAKGAALEMAAAELRAAGVE
CCHHHHHHHHHHCCCCCCEEEECCCHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
DAVLNAGGGVQVMGRHGARPWRVAIRDPFQWGVVAGVSLRPGEALHTSGNYERYFDRGGV
HHHCCCCCCEEEEECCCCCCEEEEEECCCCCCEEECCEECCCCCEECCCCHHHHHHCCCC
RFSHILDPRTGRPMQGIVSVSVLDTDGARADAAATALCIAGREAWPRVAAAMGVRAVLMI
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEE
ADDGAVFATPEMMARLEPAQGGYPAPVQIVSLPEDVTPPACPED
ECCCCEEECHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 2071578 [H]