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The map label for this gene is proP [H]

Identifier: 77461837

GI number: 77461837

Start: 6290748

End: 6292046

Strand: Reverse

Name: proP [H]

Synonym: Pfl01_5616

Alternate gene names: 77461837

Gene position: 6292046-6290748 (Counterclockwise)

Preceding gene: 77461840

Following gene: 77461836

Centisome position: 97.73

GC content: 63.13

Gene sequence:

>1299_bases
ATGACCACAGCGCCCTCGAGCCTCGCGCAGCCCGACCAACCCGCACGACCGTTGACCCGCAATGACTACAAGACTCTGTC
GCTATCGGCCCTGGGGGGCGCGCTGGAGTTCTACGATTTCATCATCTTCGTGTTCTTTGCCACCGTGGTCGGCAAATTGT
TCTTCCCGGCCGACATGCCCGAGTGGCTGCGCCTGATGCAGACTTTCGGCATCTTCGCCGCCGGTTACCTCGCGCGGCCG
CTGGGCGGGATCGTGATGGCGCACTTCGGCGACCTGCTGGGGCGCAAGAAAATGTTCACCCTGAGCATTTTCATGATGGC
CGTGCCGACCCTGATCATGGGCCTGCTGCCGACCTACGCGCAGATCGGCCTGTGGGCGCCGATTCTGTTGCTGTTGATGC
GCGTCATTCAGGGCGCGGCTATTGGCGGTGAGGTTCCGGGGGCGTGGGTCTTCGTTTCCGAACACGTGCCCGGCAAGCAC
ATCGGCTACGCCTGCGGCACTCTGACCAGCGGCCTGACGGCGGGTATCCTGCTCGGCTCGCTGGTCGCCACTGCGATCAA
CAGCATCTATACCCCAACGGAAGTCTCGGATTACGCCTGGCGGATCCCGTTCCTGTTGGGTGGCGTGTTCGGTCTGTTCT
CGGTCTACCTGCGTCGCTGGCTGCACGAAACCCCGGTGTTCGCCGAACTGCAACAGCGCAAGGCGCTGGCCGAAGAAGTG
CCGCTGCGCGCCGTGCTGCGTGACCACCGCGGGGCGATCGCGGTGTCGATGCTGCTGACCTGGCTGCTATCCGCCGGCAT
CGTCGTGGTGATCCTGATGACCCCGACCGTGCTGCAGACGGTCTATCACTTCTCGCCGACTACCGCGCTGCAATCCAACA
GCCTGGCGATCGTGTTTCTGAGCTTCGGCTGCATCATTTCCGGTGCGCTGGCGGATCGCTTTGGCGCCGGTCGCGTGTTC
GTATTCGGTTGCCTGGGCTTGCTGGCCAGTTCCTGGACGTTCTATCACAGTCTCGCCGATCACCCGGACTGGCTGTTCCC
GCTGTACGCGCTGACCGGTTTCCTGGTCGGCACCATCGGCGCGGTGCCATATGTGATGGTGAAGGCGTTTCCGCCGGTGG
TGCGCTTCAGTGGTCTGTCCTTCTCTTATAACGTCGCTTACGCGATCTTCGGCGGCCTGACCCCGATGGTCGTCAGCCTG
CTGCTGAAAGAAAGCGCGATGGGGCCTGCCTACTATGTGGCGGTGCTGTGCGGGATGGGGATCCTGGTCGGCGCGTGGTT
GTGGAGCAAAGGTCGCTAA

Upstream 100 bases:

>100_bases
CTATGTGCTTTTTCGAACAATTTGCTATGGTGCCGAACCTGCCCGAGCCACAGCCAGCGTTGTTGTGGCGGCAGATCCGA
CTATAAGAGAAGACCTTGCC

Downstream 100 bases:

>100_bases
GCCATCTTCACACTTGCCGTTACAACTGAAAGGCCAGCCTTCCGACAGGGATGCTGGCCTTTCATCCAATTGTCATATTT
CAGCCATAGAGTGTTCACAC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTAPSSLAQPDQPARPLTRNDYKTLSLSALGGALEFYDFIIFVFFATVVGKLFFPADMPEWLRLMQTFGIFAAGYLARP
LGGIVMAHFGDLLGRKKMFTLSIFMMAVPTLIMGLLPTYAQIGLWAPILLLLMRVIQGAAIGGEVPGAWVFVSEHVPGKH
IGYACGTLTSGLTAGILLGSLVATAINSIYTPTEVSDYAWRIPFLLGGVFGLFSVYLRRWLHETPVFAELQQRKALAEEV
PLRAVLRDHRGAIAVSMLLTWLLSAGIVVVILMTPTVLQTVYHFSPTTALQSNSLAIVFLSFGCIISGALADRFGAGRVF
VFGCLGLLASSWTFYHSLADHPDWLFPLYALTGFLVGTIGAVPYVMVKAFPPVVRFSGLSFSYNVAYAIFGGLTPMVVSL
LLKESAMGPAYYVAVLCGMGILVGAWLWSKGR

Sequences:

>Translated_432_residues
MTTAPSSLAQPDQPARPLTRNDYKTLSLSALGGALEFYDFIIFVFFATVVGKLFFPADMPEWLRLMQTFGIFAAGYLARP
LGGIVMAHFGDLLGRKKMFTLSIFMMAVPTLIMGLLPTYAQIGLWAPILLLLMRVIQGAAIGGEVPGAWVFVSEHVPGKH
IGYACGTLTSGLTAGILLGSLVATAINSIYTPTEVSDYAWRIPFLLGGVFGLFSVYLRRWLHETPVFAELQQRKALAEEV
PLRAVLRDHRGAIAVSMLLTWLLSAGIVVVILMTPTVLQTVYHFSPTTALQSNSLAIVFLSFGCIISGALADRFGAGRVF
VFGCLGLLASSWTFYHSLADHPDWLFPLYALTGFLVGTIGAVPYVMVKAFPPVVRFSGLSFSYNVAYAIFGGLTPMVVSL
LLKESAMGPAYYVAVLCGMGILVGAWLWSKGR
>Mature_431_residues
TTAPSSLAQPDQPARPLTRNDYKTLSLSALGGALEFYDFIIFVFFATVVGKLFFPADMPEWLRLMQTFGIFAAGYLARPL
GGIVMAHFGDLLGRKKMFTLSIFMMAVPTLIMGLLPTYAQIGLWAPILLLLMRVIQGAAIGGEVPGAWVFVSEHVPGKHI
GYACGTLTSGLTAGILLGSLVATAINSIYTPTEVSDYAWRIPFLLGGVFGLFSVYLRRWLHETPVFAELQQRKALAEEVP
LRAVLRDHRGAIAVSMLLTWLLSAGIVVVILMTPTVLQTVYHFSPTTALQSNSLAIVFLSFGCIISGALADRFGAGRVFV
FGCLGLLASSWTFYHSLADHPDWLFPLYALTGFLVGTIGAVPYVMVKAFPPVVRFSGLSFSYNVAYAIFGGLTPMVVSLL
LKESAMGPAYYVAVLCGMGILVGAWLWSKGR

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=29.3302540415704, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=30.1149425287356, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=29.8368298368298, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788942, Length=442, Percent_Identity=32.1266968325792, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87082231, Length=455, Percent_Identity=23.5164835164835, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46803; Mature: 46672

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCC
>Mature Secondary Structure 
TTAPSSLAQPDQPARPLTRNDYKTLSLSALGGALEFYDFIIFVFFATVVGKLFFPADMP
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EWLRLMQTFGIFAAGYLARPLGGIVMAHFGDLLGRKKMFTLSIFMMAVPTLIMGLLPTYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIGLWAPILLLLMRVIQGAAIGGEVPGAWVFVSEHVPGKHIGYACGTLTSGLTAGILLGS
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LVATAINSIYTPTEVSDYAWRIPFLLGGVFGLFSVYLRRWLHETPVFAELQQRKALAEEV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PLRAVLRDHRGAIAVSMLLTWLLSAGIVVVILMTPTVLQTVYHFSPTTALQSNSLAIVFL
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEHHH
SFGCIISGALADRFGAGRVFVFGCLGLLASSWTFYHSLADHPDWLFPLYALTGFLVGTIG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AVPYVMVKAFPPVVRFSGLSFSYNVAYAIFGGLTPMVVSLLLKESAMGPAYYVAVLCGMG
HHHHHHHHHCCCHHHCCCCCEEHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ILVGAWLWSKGR
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA