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The map label for this gene is rph [H]

Identifier: 77461768

GI number: 77461768

Start: 6215466

End: 6216188

Strand: Reverse

Name: rph [H]

Synonym: Pfl01_5547

Alternate gene names: 77461768

Gene position: 6216188-6215466 (Counterclockwise)

Preceding gene: 77461775

Following gene: 77461767

Centisome position: 96.55

GC content: 62.1

Gene sequence:

>723_bases
ATGAAACGTCCAAGTGGTCGCGCTGCCGATCAGCTCCGCTCGATCCGCATTACCCGCAACTACACCAAACACGCCGAGGG
ATCGGTACTGGTCGAATTCGGTGATACCAAAGTCATCTGCACCGTCAGCGTCGAGAACGGCGTGCCGCGTTTCCTCAAAG
GCCAGGGCCAGGGTTGGCTGACCGCCGAATACGGCATGTTGCCGCGCGCCACCGGCGAGCGTAACCAGCGTGAAGCGAGC
CGCGGCAAGCAGGGCGGCCGTACCCTGGAAATCCAGCGCCTGATCGGTCGTTCCCTGCGCGCCGCGCTGGACATGTCCAA
GCTGGGCGACCTCACCCTTTACGTCGACTGCGACGTGATCCAGGCTGACGGCGGTACCCGCACCGCGTCGATCACCGGCG
CCATGGTTGCGCTGGTCGATGCACTGAAAGTGATCAAGAAGCGCGGCGGCCTGAAAGGCGGCGACCCGCTCAAGCAAATG
ATCGGCGCCGTTTCCGTGGGCATGTACCAGGGCGAGCCGGTGCTGGATCTGGACTATCTTGAAGATTCCGCTGCCGAGAC
CGACCTCAATGTGGTGATGACCAGCACCGGTGGTTTCATCGAAGTGCAGGGCACTGCCGAAGGCGCGCCGTTCCAGCCTG
AAGAGTTGAACGCGATGCTGGAGCTGGCGAAGAAAGGCATGAACGAGATCTTCGAACTGCAAAAGGCTGCACTGGCCGAC
TGA

Upstream 100 bases:

>100_bases
GGGCCTTCGGTCGTCGGCCGGATTTTTTAACGAGTGCCCAGGCGCGGTGCACAGCTCTATAATGCTCCGCAGTTTTCCGT
CCCCAGTACAGGTATCTCCT

Downstream 100 bases:

>100_bases
GCAGGTTCGTAACCCGCAAGGAGGACACGATGAGTGATGAGCAAGAGTTGCTGCCCAAGCCGAGCCAGGAGGTTCGCCAA
TGGGCCATGTTTTGTCACTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWLTAEYGMLPRATGERNQREAS
RGKQGGRTLEIQRLIGRSLRAALDMSKLGDLTLYVDCDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQM
IGAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD

Sequences:

>Translated_240_residues
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWLTAEYGMLPRATGERNQREAS
RGKQGGRTLEIQRLIGRSLRAALDMSKLGDLTLYVDCDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQM
IGAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD
>Mature_240_residues
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWLTAEYGMLPRATGERNQREAS
RGKQGGRTLEIQRLIGRSLRAALDMSKLGDLTLYVDCDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQM
IGAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=70.8133971291866, Blast_Score=311, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71981632, Length=233, Percent_Identity=26.1802575107296, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25805; Mature: 25805

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWL
CCCCCCCHHHHHHHHHEECCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHCCCCCCEE
TAEYGMLPRATGERNQREASRGKQGGRTLEIQRLIGRSLRAALDMSKLGDLTLYVDCDVI
EECCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
QADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIGAVSVGMYQGEPVLDLDYL
ECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHC
EDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD
CCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRPSGRAADQLRSIRITRNYTKHAEGSVLVEFGDTKVICTVSVENGVPRFLKGQGQGWL
CCCCCCCHHHHHHHHHEECCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHCCCCCCEE
TAEYGMLPRATGERNQREASRGKQGGRTLEIQRLIGRSLRAALDMSKLGDLTLYVDCDVI
EECCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
QADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIGAVSVGMYQGEPVLDLDYL
ECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHC
EDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD
CCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA