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The map label for this gene is pyrE [H]

Identifier: 77461765

GI number: 77461765

Start: 6213498

End: 6214142

Strand: Reverse

Name: pyrE [H]

Synonym: Pfl01_5544

Alternate gene names: 77461765

Gene position: 6214142-6213498 (Counterclockwise)

Preceding gene: 77461767

Following gene: 77461760

Centisome position: 96.52

GC content: 62.64

Gene sequence:

>645_bases
ATGCAAGCGTATCAGCGCGATTTCATTCGTTTTGCCATCGATCGCGGCGTTTTGCGCTTCGGTGAGTTCACCCTGAAGTC
CGGGCGTACCAGTCCTTACTTCTTCAATGCGGGCCTGTTCAACTCGGGTTCCGCGCTGGCGCAGCTGGGTCGTTTCTACG
CCGCAGCCATCGCCGAGAGCGGCATTCCGTTCGACGTGCTTTTCGGCCCGGCCTACAAAGGCATCCCGCTGGCGGCAACC
ACTGCCGTGGCACTGGCCGAACACCACAACCGTGACTTGCCATGGTGCTTCAACCGCAAGGAAGCCAAGGCCCACGGCGA
GGGTGGCAGCCTGGTCGGCGCACCGCTGACCGGCGACGTGCTGATCATCGACGACGTGATCACCGCCGGCACCGCGATCC
GTGAAGTGATGCAGATCATCGCTTCCCAGGACGGCGCCAAGGCCGCCGGCGTGCTGATCGCCCTGAACCGTCAGGAGCGC
GGCAACGGCGAGCTGTCGGCAATCCAGGAAGTCGAGCGTGATTTTGGCATCCCGGTCATCAGCATCGTGTCGCTGAACCA
GGTACTGGAATTCCTGGCAGACGACCCGCAACTCAAGCAGCACCTGCCAGCCGTTGAAGCCTATCGCGCCCAGTTCGGCG
TCTGA

Upstream 100 bases:

>100_bases
CACACTGATGATCCGCATAAATGGCCCCAAAAATCGCGTGCGTGTATGATACACGGCGTCAAGCTAATTAGCTAAATCCG
TGGTATTTGGGGTTTTTTTC

Downstream 100 bases:

>100_bases
TCGCTTCTTCAAGGGCAAAAAAAGACCCCGCTCAGCCAGGCTGAGCGGGGTCTTCTTGTTTGCGCTATCGCTTACGGACG
CTTGCGATTGCTGATCAGCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNSGSALAQLGRFYAAAIAESGIPFDVLFGPAYKGIPLAAT
TAVALAEHHNRDLPWCFNRKEAKAHGEGGSLVGAPLTGDVLIIDDVITAGTAIREVMQIIASQDGAKAAGVLIALNRQER
GNGELSAIQEVERDFGIPVISIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGV

Sequences:

>Translated_214_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNSGSALAQLGRFYAAAIAESGIPFDVLFGPAYKGIPLAAT
TAVALAEHHNRDLPWCFNRKEAKAHGEGGSLVGAPLTGDVLIIDDVITAGTAIREVMQIIASQDGAKAAGVLIALNRQER
GNGELSAIQEVERDFGIPVISIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGV
>Mature_214_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNSGSALAQLGRFYAAAIAESGIPFDVLFGPAYKGIPLAAT
TAVALAEHHNRDLPWCFNRKEAKAHGEGGSLVGAPLTGDVLIIDDVITAGTAIREVMQIIASQDGAKAAGVLIALNRQER
GNGELSAIQEVERDFGIPVISIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=181, Percent_Identity=28.7292817679558, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI1790073, Length=214, Percent_Identity=67.7570093457944, Blast_Score=307, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17554964, Length=192, Percent_Identity=28.125, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508631, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=200, Percent_Identity=52.5, Blast_Score=197, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6323927, Length=222, Percent_Identity=48.1981981981982, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23063; Mature: 23063

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNSGSALAQLGRFYAAAIAES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIPFDVLFGPAYKGIPLAATTAVALAEHHNRDLPWCFNRKEAKAHGEGGSLVGAPLTGDV
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCE
LIIDDVITAGTAIREVMQIIASQDGAKAAGVLIALNRQERGNGELSAIQEVERDFGIPVI
EEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
SIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGV
HHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNSGSALAQLGRFYAAAIAES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIPFDVLFGPAYKGIPLAATTAVALAEHHNRDLPWCFNRKEAKAHGEGGSLVGAPLTGDV
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCE
LIIDDVITAGTAIREVMQIIASQDGAKAAGVLIALNRQERGNGELSAIQEVERDFGIPVI
EEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
SIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGV
HHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA