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The map label for this gene is lutR [H]

Identifier: 77459941

GI number: 77459941

Start: 4204208

End: 4204921

Strand: Reverse

Name: lutR [H]

Synonym: Pfl01_3719

Alternate gene names: 77459941

Gene position: 4204921-4204208 (Counterclockwise)

Preceding gene: 77459944

Following gene: 77459939

Centisome position: 65.31

GC content: 62.32

Gene sequence:

>714_bases
ATGCAAGACGACATCGACGCCCCCGCCCGCAAACGCGCGCACAACCTGGCCCATGACCTGGTGGAAAAACTGACCCAGAG
CATCCTGCTCGGTCAGATGCTGCCCGGTGACAAGTTGCCGTCGGAAAACTCGATCGTTCAGGAACACGGCGTCAGCCGCA
CCGTCGTGCGCGAGGCGATTTCCAAATTGCAGGCGTCGGGGCTGGTGGAGACGCGGCACGGCATCGGCACGTTCGTGATC
GAACGCGCACAGGAGCAGGGGTTGCGACTGAATGTCGACACCGCGCTGGGGGTGCGCAGCATTCTCGAATTGCGCATGGG
GCTGGAGACGCAAGCGGCGGCGCTGGCCGCGCAGCGCCGCACCGAACAGCAGCTGGAGCAGATGCGTCAGGCACTCGATG
ACTATCAGCGTCTATTGGCCAACAACGACAGCTGCGTTGAAGCCGACCGGCGCTTCCACCTGTTGATTGCCGAAGCCACC
GGCAATGTCTGCTTCACCGAAATTATGCAGCACTTGGGCAGCGCAATGATTCCGCGCACTCGGGTCAACGCGGCCGAGCG
CGGGGCGGTGGATTTGAGCAAGCTCGGGCAACTGGCGAACCTGGAACATGAGGCGATTTTCAACGCAATCAAACGCCAGG
ATTCGGAAGCGGCGCGTGCGGCGATGTGGCTGCACCTGAGCAATAGTCGCGACCGGTTTTCGGCTCAGGGGTAA

Upstream 100 bases:

>100_bases
GAGTGTTGTCATACAAGCTGAATCCCGGAATTCATAGGAGCTCATACAAGTCATTGCCGCCAGTGCTACGATCGGTCTGC
CCCGATCACCGGAACCGACC

Downstream 100 bases:

>100_bases
ACCAAAGGAATAACGCGGAGCTTTCTCAGTCCTTATCCACATCGCGCATCAATAACCCGAACCGCAGATCCACCGCATCC
GGAATCGGCAGATACACCGT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MQDDIDAPARKRAHNLAHDLVEKLTQSILLGQMLPGDKLPSENSIVQEHGVSRTVVREAISKLQASGLVETRHGIGTFVI
ERAQEQGLRLNVDTALGVRSILELRMGLETQAAALAAQRRTEQQLEQMRQALDDYQRLLANNDSCVEADRRFHLLIAEAT
GNVCFTEIMQHLGSAMIPRTRVNAAERGAVDLSKLGQLANLEHEAIFNAIKRQDSEAARAAMWLHLSNSRDRFSAQG

Sequences:

>Translated_237_residues
MQDDIDAPARKRAHNLAHDLVEKLTQSILLGQMLPGDKLPSENSIVQEHGVSRTVVREAISKLQASGLVETRHGIGTFVI
ERAQEQGLRLNVDTALGVRSILELRMGLETQAAALAAQRRTEQQLEQMRQALDDYQRLLANNDSCVEADRRFHLLIAEAT
GNVCFTEIMQHLGSAMIPRTRVNAAERGAVDLSKLGQLANLEHEAIFNAIKRQDSEAARAAMWLHLSNSRDRFSAQG
>Mature_237_residues
MQDDIDAPARKRAHNLAHDLVEKLTQSILLGQMLPGDKLPSENSIVQEHGVSRTVVREAISKLQASGLVETRHGIGTFVI
ERAQEQGLRLNVDTALGVRSILELRMGLETQAAALAAQRRTEQQLEQMRQALDDYQRLLANNDSCVEADRRFHLLIAEAT
GNVCFTEIMQHLGSAMIPRTRVNAAERGAVDLSKLGQLANLEHEAIFNAIKRQDSEAARAAMWLHLSNSRDRFSAQG

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=208, Percent_Identity=34.6153846153846, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI87082208, Length=235, Percent_Identity=31.9148936170213, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI48994955, Length=231, Percent_Identity=29.4372294372294, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790032, Length=200, Percent_Identity=32.5, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994961, Length=218, Percent_Identity=31.1926605504587, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789621, Length=246, Percent_Identity=27.6422764227642, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786303, Length=218, Percent_Identity=29.8165137614679, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26276; Mature: 26276

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDDIDAPARKRAHNLAHDLVEKLTQSILLGQMLPGDKLPSENSIVQEHGVSRTVVREAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHH
SKLQASGLVETRHGIGTFVIERAQEQGLRLNVDTALGVRSILELRMGLETQAAALAAQRR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TEQQLEQMRQALDDYQRLLANNDSCVEADRRFHLLIAEATGNVCFTEIMQHLGSAMIPRT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
RVNAAERGAVDLSKLGQLANLEHEAIFNAIKRQDSEAARAAMWLHLSNSRDRFSAQG
HCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCC
>Mature Secondary Structure
MQDDIDAPARKRAHNLAHDLVEKLTQSILLGQMLPGDKLPSENSIVQEHGVSRTVVREAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHH
SKLQASGLVETRHGIGTFVIERAQEQGLRLNVDTALGVRSILELRMGLETQAAALAAQRR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TEQQLEQMRQALDDYQRLLANNDSCVEADRRFHLLIAEATGNVCFTEIMQHLGSAMIPRT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
RVNAAERGAVDLSKLGQLANLEHEAIFNAIKRQDSEAARAAMWLHLSNSRDRFSAQG
HCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]