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The map label for this gene is aroE [C]

Identifier: 77459935

GI number: 77459935

Start: 4199478

End: 4200296

Strand: Reverse

Name: aroE [C]

Synonym: Pfl01_3713

Alternate gene names: 77459935

Gene position: 4200296-4199478 (Counterclockwise)

Preceding gene: 77459939

Following gene: 77459934

Centisome position: 65.24

GC content: 60.93

Gene sequence:

>819_bases
ATGCAGATGAACCCCAACAAAGACACCCAATTGTGCATGTCCCTGTCTGGGCGTCCCGGGAATTTCGGTCTGCGTTTTCA
TAACCATTTGTATGAACAACTGGGCCTGAATTTCTACTACAAGGCATTCAGCAGCCAGGACCTGCCCGGTGCGGTCGGCG
GGATCCGCGCACTGGGGATTCGCGGTTGCGGCGTGTCGATGCCGTTCAAGGAAGCCAGCATTGCGCTGGTCGATGAGCTG
GATGCGTCGGCGGCAGCGATTCAGTCGATCAACACCATCGTCAACACCAACGGCCATCTCAAGGCCTACAACACCGATTA
CATTGCCATCGCCCAGTTGCTGGAAACCCACGCGGTGCCCAAGGACACGACTTTCGCGCTGCGCGGCAGCGGCGGTATGG
CCAAGGCGGTGGCCAGTGCCTTGCGCGATGACGGTTACAAAAACGGTTTGATCGTCGCCCGCAACGAGCGCGCCGGCCGT
GCGCTGGCGGATTCTTTGGGTTATCGCTGGCAGGCAGAACTGGGTGACGAGCGCCCGCAGATGCTGATCAACGTCACGCC
GGTGGGGATGGACGGTGGCCCGGAAGCAGGGCAGCTTTCCTTTGACGTGGATGTGATCAAGTCTGCCGACACCGTGTTCG
ATGTGGTGGCGATCCCCTCGGAAACCCCGCTGATCGTGCGCGGTCGTGCCGAAGGCAAAAAAGTGATCACCGGGCTGGAA
GTGATCGCGATCCAGGCGCTGGAGCAGTTCGTGCTGTACACCGGCGTGCGGCCGACGGTCGAGCAGTTCGATGCGGCGGT
GGCGTTTGCCCGCAGTTGA

Upstream 100 bases:

>100_bases
CATCGGCTGTGATGCCCGGAACAGACCGCCTTATGATCGTTCAGAACCCGATGGCCTGGCTAATCCCGTCCATCCGAAAG
TGCATTCGAAGGAGCCTCCC

Downstream 100 bases:

>100_bases
TGGGGCCTGAGCAACGGCGAACCCACCCGGATTTGCCGTTGCCTATACTGCCGGATCAGCAAGCACAGACTTGCCCTTCA
CAAAAGCCGAGGTTTGCATG

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MQMNPNKDTQLCMSLSGRPGNFGLRFHNHLYEQLGLNFYYKAFSSQDLPGAVGGIRALGIRGCGVSMPFKEASIALVDEL
DASAAAIQSINTIVNTNGHLKAYNTDYIAIAQLLETHAVPKDTTFALRGSGGMAKAVASALRDDGYKNGLIVARNERAGR
ALADSLGYRWQAELGDERPQMLINVTPVGMDGGPEAGQLSFDVDVIKSADTVFDVVAIPSETPLIVRGRAEGKKVITGLE
VIAIQALEQFVLYTGVRPTVEQFDAAVAFARS

Sequences:

>Translated_272_residues
MQMNPNKDTQLCMSLSGRPGNFGLRFHNHLYEQLGLNFYYKAFSSQDLPGAVGGIRALGIRGCGVSMPFKEASIALVDEL
DASAAAIQSINTIVNTNGHLKAYNTDYIAIAQLLETHAVPKDTTFALRGSGGMAKAVASALRDDGYKNGLIVARNERAGR
ALADSLGYRWQAELGDERPQMLINVTPVGMDGGPEAGQLSFDVDVIKSADTVFDVVAIPSETPLIVRGRAEGKKVITGLE
VIAIQALEQFVLYTGVRPTVEQFDAAVAFARS
>Mature_272_residues
MQMNPNKDTQLCMSLSGRPGNFGLRFHNHLYEQLGLNFYYKAFSSQDLPGAVGGIRALGIRGCGVSMPFKEASIALVDEL
DASAAAIQSINTIVNTNGHLKAYNTDYIAIAQLLETHAVPKDTTFALRGSGGMAKAVASALRDDGYKNGLIVARNERAGR
ALADSLGYRWQAELGDERPQMLINVTPVGMDGGPEAGQLSFDVDVIKSADTVFDVVAIPSETPLIVRGRAEGKKVITGLE
VIAIQALEQFVLYTGVRPTVEQFDAAVAFARS

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=265, Percent_Identity=32.4528301886792, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29150; Mature: 29150

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMNPNKDTQLCMSLSGRPGNFGLRFHNHLYEQLGLNFYYKAFSSQDLPGAVGGIRALGI
CCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHEEEE
RGCGVSMPFKEASIALVDELDASAAAIQSINTIVNTNGHLKAYNTDYIAIAQLLETHAVP
ECCCCCCCCCCCCEEEEHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCC
KDTTFALRGSGGMAKAVASALRDDGYKNGLIVARNERAGRALADSLGYRWQAELGDERPQ
CCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCCCE
MLINVTPVGMDGGPEAGQLSFDVDVIKSADTVFDVVAIPSETPLIVRGRAEGKKVITGLE
EEEEEEEECCCCCCCCCEEEEEHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHH
VIAIQALEQFVLYTGVRPTVEQFDAAVAFARS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQMNPNKDTQLCMSLSGRPGNFGLRFHNHLYEQLGLNFYYKAFSSQDLPGAVGGIRALGI
CCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHEEEE
RGCGVSMPFKEASIALVDELDASAAAIQSINTIVNTNGHLKAYNTDYIAIAQLLETHAVP
ECCCCCCCCCCCCEEEEHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCC
KDTTFALRGSGGMAKAVASALRDDGYKNGLIVARNERAGRALADSLGYRWQAELGDERPQ
CCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCCCE
MLINVTPVGMDGGPEAGQLSFDVDVIKSADTVFDVVAIPSETPLIVRGRAEGKKVITGLE
EEEEEEEECCCCCCCCCEEEEEHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHH
VIAIQALEQFVLYTGVRPTVEQFDAAVAFARS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]