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The map label for this gene is nuoI [H]

Identifier: 77459832

GI number: 77459832

Start: 4081639

End: 4082187

Strand: Direct

Name: nuoI [H]

Synonym: Pfl01_3610

Alternate gene names: 77459832

Gene position: 4081639-4082187 (Clockwise)

Preceding gene: 77459831

Following gene: 77459833

Centisome position: 63.4

GC content: 58.29

Gene sequence:

>549_bases
ATGTTCAAATATATTGGCGACATCGTTAAGGGTACCGGTACCCAGTTGCGCAGCCTGGTCATGGTCTTCGGCCATGGCTT
TCGCAAGCGCGACACCCTGCAATACCCGGAAGAGCAGGTCTACCTGCCGCCACGCTATCGTGGCCGCATCGTCCTGACCC
GCGACCCCGATGGCGAAGAACGCTGCGTAGCCTGCAACCTGTGCGCCGTGGCCTGCCCGGTGGGTTGCATCTCGCTGCAG
AAAGCTGAAACCGAAGACGGTCGCTGGTACCCGGACTTCTTCCGTATCAACTTCTCGCGCTGCATTTTCTGCGGTCTCTG
CGAGGAAGCCTGCCCGACCACCGCGATCCAGCTGACCCCGGATTTCGAGATGGCCGAGTTCAAACGTCAGGACCTGGTGT
ACGAGAAAGAAGATCTTTTGATCTCCGGCCCCGGCAAAAACCCTGATTACAACTTCTATCGTGTTGCAGGTATGGCCGTT
GCGGGCAAGCCGAAAGGCGCCGCACAAAACGAAGCCGAGCCGATCAACGTGAAGAGCTTGCTGCCTTAA

Upstream 100 bases:

>100_bases
GGAAATTCTGCCTGCCACTGACCCTGATCAATTTGCTGGTGACCGCTGCAATCGTGTTGTGGAACACGCCTGCGGTTGCG
GTTCAGTGAGGATTTGACCC

Downstream 100 bases:

>100_bases
GGAAGAACGATGGAATTCGCTTTCTATTTCGCATCGGGTATCGCTGTTGTGTCCACACTGCGTGTGGTCACCAACACCAA
TCCTGTGCACGCCCTGCTCT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MFKYIGDIVKGTGTQLRSLVMVFGHGFRKRDTLQYPEEQVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQ
KAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMAEFKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAV
AGKPKGAAQNEAEPINVKSLLP

Sequences:

>Translated_182_residues
MFKYIGDIVKGTGTQLRSLVMVFGHGFRKRDTLQYPEEQVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQ
KAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMAEFKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAV
AGKPKGAAQNEAEPINVKSLLP
>Mature_182_residues
MFKYIGDIVKGTGTQLRSLVMVFGHGFRKRDTLQYPEEQVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQ
KAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMAEFKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAV
AGKPKGAAQNEAEPINVKSLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=128, Percent_Identity=41.40625, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1788617, Length=178, Percent_Identity=81.4606741573034, Blast_Score=307, Evalue=3e-85,
Organism=Escherichia coli, GI1789075, Length=135, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=134, Percent_Identity=39.5522388059701, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864306, Length=131, Percent_Identity=41.2213740458015, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20503; Mature: 20503

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKYIGDIVKGTGTQLRSLVMVFGHGFRKRDTLQYPEEQVYLPPRYRGRIVLTRDPDGEE
CCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCC
RCVACNLCAVACPVGCISLQKAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTP
EEEEEHHHHHHCCCCCEEEECCCCCCCCCCCHHEEEEHHHEEEEEHHHHCCCCCEEEECC
DFEMAEFKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAVAGKPKGAAQNEAEPINVKSL
CCHHHHHHHHHCCEECCCEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHCC
LP
CC
>Mature Secondary Structure
MFKYIGDIVKGTGTQLRSLVMVFGHGFRKRDTLQYPEEQVYLPPRYRGRIVLTRDPDGEE
CCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCC
RCVACNLCAVACPVGCISLQKAETEDGRWYPDFFRINFSRCIFCGLCEEACPTTAIQLTP
EEEEEHHHHHHCCCCCEEEECCCCCCCCCCCHHEEEEHHHEEEEEHHHHCCCCCEEEECC
DFEMAEFKRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAVAGKPKGAAQNEAEPINVKSL
CCHHHHHHHHHCCEECCCEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHCC
LP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA