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The map label for this gene is aprF [H]

Identifier: 77458907

GI number: 77458907

Start: 3083608

End: 3084963

Strand: Direct

Name: aprF [H]

Synonym: Pfl01_2682

Alternate gene names: 77458907

Gene position: 3083608-3084963 (Clockwise)

Preceding gene: 77458906

Following gene: 77458908

Centisome position: 47.89

GC content: 60.91

Gene sequence:

>1356_bases
ATGAATAAGCTTTCCATGCTGGCTGCAGCGCTCGCGCTGCTTGCGGGCAACAGTGCAGTGGCGGCCATGGGGCCGTTCGA
GATCTACGAACAGGCGTTGCGCAATGACCCGGTGTTCCTCGGCGCGATCAAGGAGCGCGACGCCGGCCTTGAAAACCGCG
CCATCGGCCGCGCCGGCCTGTTGCCGAAACTGGGTTACAACTACAACAAGGGTCGCAACTCGTCGAAGGCCACGTCCCTC
GACGAACGCGCGCGCAATCGCACCGATGAGCGTAATTACAACAGCTACGGCTCGAGCCTGACCCTGCAACAACCGTTGCT
CGACTACGAAGCCTACGCGGCCTATCGCAAGGGCGTGGCCCAGTCACTGTTTGCCGACGAAAACTTTCGCGGCAAGAGCC
AGGAGCTTCTGGTTCGAGTACTGGATAACTACACCAAGGCCTTGTTTGCCCAGGATCAGATCGACATCGCGCAGGCCAAG
AAGAAAGCGTACGAGCAGCAGTTCCAGCAGAACGAGCACATGTTCAAGCAGGGCGAGGGTACTCGTACGGACATCCTTGA
AGCCGAGTCGCGTTACGAACTGGCGACCGCCGAGGAAATCGAGGCCCGCGACGAGCAGGATGCCGCGCTACGGGAGCTGG
GCGCATTGGTAGGCGTGCCGGCGATGGACATCAGTGACCTGGCCCCGTTGGATCAGAACTTCCAGACCTTTGCCTTGATG
CCGGCCAACTACGACACCTGGCACGAGCTGGCGGTCAGCAATAACCCGAACCTTGCGTCCCAGCGTCAGGCGGTTGAAGT
GGCGCGGTACGAAGTCGAGCGCAACCGCGCCGGGCACCTGCCGAAAGTCAGCGCTTACGCCACGATGCGCCAGAACGAGT
CGGAAAGCGGCAACACCTACAACCAGCGTTACGAAACCAACACCATCGGTTTCGAAGTCAGCGTGCCGTTGTATGCCGGT
GGCGGCGTGTCGGCTTCGACCCGTCAGGCCAGCCGCACCATGGAACAGGCCGAATACGAACTGGACGGCAAGACCCGCGA
GACGCTGATCGAATTGCGCCGGCAGTTCAGTGCGTGCCTGTCCGGGGTCAATAAATTGCGCGCCTATCAAAAGGCCCTGG
CCTCGGCCGAAGCGCTGGTGGTGTCGACCAAACAGAGCATTCTCGGCGGCGAGCGCACCAACCTCGACGCCCTGAACGCC
GAACAACAGCTGTTTACCACCCGCCGTGATCTTGCCCAGGCCCGCTACGACTACCTTATGGCTTGGACCAAATTGCATTA
CTACGCGGGCACCTTGAGCGAACAGGATTTGGCGCGGGTGGATGAGGCGTTCGGACAGGGTCCACGGATGCAATAA

Upstream 100 bases:

>100_bases
TCGTGCGCACTGGCGAACGTTCACTCCTCAACTACCTGTTCAAGCCGCTGCTCGACCGCGCCGGTTCCGCGTTGACCGAA
GAATAAGGATGTTCGGCTGT

Downstream 100 bases:

>100_bases
CACCGTAGCTTCAACGCAAAAAGCTTCGACCCAAAACAACAAAAAGAGTGGTGAACATGGACGTACGCATCAAGCCGATT
TCGGTCGGCACCCTGCTGCT

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNKLSMLAAALALLAGNSAVAAMGPFEIYEQALRNDPVFLGAIKERDAGLENRAIGRAGLLPKLGYNYNKGRNSSKATSL
DERARNRTDERNYNSYGSSLTLQQPLLDYEAYAAYRKGVAQSLFADENFRGKSQELLVRVLDNYTKALFAQDQIDIAQAK
KKAYEQQFQQNEHMFKQGEGTRTDILEAESRYELATAEEIEARDEQDAALRELGALVGVPAMDISDLAPLDQNFQTFALM
PANYDTWHELAVSNNPNLASQRQAVEVARYEVERNRAGHLPKVSAYATMRQNESESGNTYNQRYETNTIGFEVSVPLYAG
GGVSASTRQASRTMEQAEYELDGKTRETLIELRRQFSACLSGVNKLRAYQKALASAEALVVSTKQSILGGERTNLDALNA
EQQLFTTRRDLAQARYDYLMAWTKLHYYAGTLSEQDLARVDEAFGQGPRMQ

Sequences:

>Translated_451_residues
MNKLSMLAAALALLAGNSAVAAMGPFEIYEQALRNDPVFLGAIKERDAGLENRAIGRAGLLPKLGYNYNKGRNSSKATSL
DERARNRTDERNYNSYGSSLTLQQPLLDYEAYAAYRKGVAQSLFADENFRGKSQELLVRVLDNYTKALFAQDQIDIAQAK
KKAYEQQFQQNEHMFKQGEGTRTDILEAESRYELATAEEIEARDEQDAALRELGALVGVPAMDISDLAPLDQNFQTFALM
PANYDTWHELAVSNNPNLASQRQAVEVARYEVERNRAGHLPKVSAYATMRQNESESGNTYNQRYETNTIGFEVSVPLYAG
GGVSASTRQASRTMEQAEYELDGKTRETLIELRRQFSACLSGVNKLRAYQKALASAEALVVSTKQSILGGERTNLDALNA
EQQLFTTRRDLAQARYDYLMAWTKLHYYAGTLSEQDLARVDEAFGQGPRMQ
>Mature_451_residues
MNKLSMLAAALALLAGNSAVAAMGPFEIYEQALRNDPVFLGAIKERDAGLENRAIGRAGLLPKLGYNYNKGRNSSKATSL
DERARNRTDERNYNSYGSSLTLQQPLLDYEAYAAYRKGVAQSLFADENFRGKSQELLVRVLDNYTKALFAQDQIDIAQAK
KKAYEQQFQQNEHMFKQGEGTRTDILEAESRYELATAEEIEARDEQDAALRELGALVGVPAMDISDLAPLDQNFQTFALM
PANYDTWHELAVSNNPNLASQRQAVEVARYEVERNRAGHLPKVSAYATMRQNESESGNTYNQRYETNTIGFEVSVPLYAG
GGVSASTRQASRTMEQAEYELDGKTRETLIELRRQFSACLSGVNKLRAYQKALASAEALVVSTKQSILGGERTNLDALNA
EQQLFTTRRDLAQARYDYLMAWTKLHYYAGTLSEQDLARVDEAFGQGPRMQ

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=438, Percent_Identity=26.4840182648402, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50367; Mature: 50367

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLSMLAAALALLAGNSAVAAMGPFEIYEQALRNDPVFLGAIKERDAGLENRAIGRAGL
CCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEHHHHCCCCCHHCCCCCCC
LPKLGYNYNKGRNSSKATSLDERARNRTDERNYNSYGSSLTLQQPLLDYEAYAAYRKGVA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
QSLFADENFRGKSQELLVRVLDNYTKALFAQDQIDIAQAKKKAYEQQFQQNEHMFKQGEG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TRTDILEAESRYELATAEEIEARDEQDAALRELGALVGVPAMDISDLAPLDQNFQTFALM
CHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEEE
PANYDTWHELAVSNNPNLASQRQAVEVARYEVERNRAGHLPKVSAYATMRQNESESGNTY
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCH
NQRYETNTIGFEVSVPLYAGGGVSASTRQASRTMEQAEYELDGKTRETLIELRRQFSACL
HHCEECCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SGVNKLRAYQKALASAEALVVSTKQSILGGERTNLDALNAEQQLFTTRRDLAQARYDYLM
HHHHHHHHHHHHHHHHHHHEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AWTKLHYYAGTLSEQDLARVDEAFGQGPRMQ
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKLSMLAAALALLAGNSAVAAMGPFEIYEQALRNDPVFLGAIKERDAGLENRAIGRAGL
CCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEHHHHCCCCCHHCCCCCCC
LPKLGYNYNKGRNSSKATSLDERARNRTDERNYNSYGSSLTLQQPLLDYEAYAAYRKGVA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
QSLFADENFRGKSQELLVRVLDNYTKALFAQDQIDIAQAKKKAYEQQFQQNEHMFKQGEG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TRTDILEAESRYELATAEEIEARDEQDAALRELGALVGVPAMDISDLAPLDQNFQTFALM
CHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEEE
PANYDTWHELAVSNNPNLASQRQAVEVARYEVERNRAGHLPKVSAYATMRQNESESGNTY
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCH
NQRYETNTIGFEVSVPLYAGGGVSASTRQASRTMEQAEYELDGKTRETLIELRRQFSACL
HHCEECCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SGVNKLRAYQKALASAEALVVSTKQSILGGERTNLDALNAEQQLFTTRRDLAQARYDYLM
HHHHHHHHHHHHHHHHHHHEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AWTKLHYYAGTLSEQDLARVDEAFGQGPRMQ
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]