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The map label for this gene is potF [H]

Identifier: 77458902

GI number: 77458902

Start: 3076797

End: 3077882

Strand: Direct

Name: potF [H]

Synonym: Pfl01_2677

Alternate gene names: 77458902

Gene position: 3076797-3077882 (Clockwise)

Preceding gene: 77458901

Following gene: 77458903

Centisome position: 47.79

GC content: 58.93

Gene sequence:

>1086_bases
ATGATCCGAAAGACCCTCGCGCTCGCACCCTTGATGCTCGCCGTCTCCTTCGCTCAGGCGGCGGATACGGTCAAGGTTTA
CAACTGGTCCGACTACATCGCGCCGGACACCGCCAAGAACTTCGAAAAGGCCAGCGGCATCGGCGTCACCTATGACGTCT
ACGACAGCAACGAAACCCTCGACGGCAAGCTGATGACCGGCAAATCCGGTTACGACGTGGTGTTTCCCTCCAACCACTTC
ATGGCGCGGCAGATCGAGGGCGGGGCACTGAAGAAACTCGACAAGAGCCAGTTGCCGAACTGGAAGAACCTCAACCCGGT
ATTGCTCAAGGCCTTGCAGACCAACGATCCGGGTAACGAACACGGTTTTCCGTACCTGTGGGGCAGCACTGGCATCGGCT
ACAACGTCGCCAAGGTCAAGGCCGTGCTGGGTGACAACGCGCCGGTGGATTCCTGGGATCTGATCTTCAAACCCGAATAC
ATGGAAAAACTGCAGAAGTGCGGCGTGGCCATCCTCGACAACGGCCCGGAACTGCTGCCGGCGGCGCTCAACTACCTGGG
TTTGCCGCATCACAGCAAGAACCCCGAGGACTACAAGAAAGCTGAAGCACTGCTGATGAAAGTGCGGCCGTACGTCAGCT
ATTTCCATTCCTCGAAGTACACCAGCGATCTGGCCAATGGCGACATTTGCGTGGCCGTCGGTTTCTCCGGCGACATCCTG
CAAGCGGAAAACCGCGCCAAAGAAGCGAAGAACGGCATCGACATCGGCTACGAGATTCCCAAGGAAGGCGCCGCCATCTG
GTTCGACATGGTCGCCATGCCGGCCGACGCGCCGGACGAGAAGGCCGGCTACGCGTTCATGAACTACCTGCTGCAGCCCG
ACGTGATGGCCGGCATCAGCAACTACGTGCATTACGCCAATGGCAACGAACAGGCTGACAGCCTGATCGACCCGGCGATC
AAGAACGACACCAAGGTGTATCCGAGCCCGGAAATGATGGGCAAGCTGTTCGCACTGGAAGCGATGCCGCTGAACATCGA
CCGGATCCGCACGCGGGTGTGGAACAAGATTCGTACTGGCAGCTGA

Upstream 100 bases:

>100_bases
ACATCGAAGAAACCGTGCGCAAGACCTATGTCCTGATTTTCTGACGTTTTGATCCTTTTGATTGCCTGCCAACAACAATA
GCCAATACAGGAATCGATCC

Downstream 100 bases:

>100_bases
AGAAAAACAGGCGAGGGCGGCACCACAGCCTTCGCCTGATTATGTTCTGTCGGAATTACTTGATATCAATACACTGGCAT
CGCACTGATAAAGTGCAGCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIRKTLALAPLMLAVSFAQAADTVKVYNWSDYIAPDTAKNFEKASGIGVTYDVYDSNETLDGKLMTGKSGYDVVFPSNHF
MARQIEGGALKKLDKSQLPNWKNLNPVLLKALQTNDPGNEHGFPYLWGSTGIGYNVAKVKAVLGDNAPVDSWDLIFKPEY
MEKLQKCGVAILDNGPELLPAALNYLGLPHHSKNPEDYKKAEALLMKVRPYVSYFHSSKYTSDLANGDICVAVGFSGDIL
QAENRAKEAKNGIDIGYEIPKEGAAIWFDMVAMPADAPDEKAGYAFMNYLLQPDVMAGISNYVHYANGNEQADSLIDPAI
KNDTKVYPSPEMMGKLFALEAMPLNIDRIRTRVWNKIRTGS

Sequences:

>Translated_361_residues
MIRKTLALAPLMLAVSFAQAADTVKVYNWSDYIAPDTAKNFEKASGIGVTYDVYDSNETLDGKLMTGKSGYDVVFPSNHF
MARQIEGGALKKLDKSQLPNWKNLNPVLLKALQTNDPGNEHGFPYLWGSTGIGYNVAKVKAVLGDNAPVDSWDLIFKPEY
MEKLQKCGVAILDNGPELLPAALNYLGLPHHSKNPEDYKKAEALLMKVRPYVSYFHSSKYTSDLANGDICVAVGFSGDIL
QAENRAKEAKNGIDIGYEIPKEGAAIWFDMVAMPADAPDEKAGYAFMNYLLQPDVMAGISNYVHYANGNEQADSLIDPAI
KNDTKVYPSPEMMGKLFALEAMPLNIDRIRTRVWNKIRTGS
>Mature_361_residues
MIRKTLALAPLMLAVSFAQAADTVKVYNWSDYIAPDTAKNFEKASGIGVTYDVYDSNETLDGKLMTGKSGYDVVFPSNHF
MARQIEGGALKKLDKSQLPNWKNLNPVLLKALQTNDPGNEHGFPYLWGSTGIGYNVAKVKAVLGDNAPVDSWDLIFKPEY
MEKLQKCGVAILDNGPELLPAALNYLGLPHHSKNPEDYKKAEALLMKVRPYVSYFHSSKYTSDLANGDICVAVGFSGDIL
QAENRAKEAKNGIDIGYEIPKEGAAIWFDMVAMPADAPDEKAGYAFMNYLLQPDVMAGISNYVHYANGNEQADSLIDPAI
KNDTKVYPSPEMMGKLFALEAMPLNIDRIRTRVWNKIRTGS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=357, Percent_Identity=54.3417366946779, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787367, Length=322, Percent_Identity=32.9192546583851, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787711, Length=301, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39818; Mature: 39818

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKTLALAPLMLAVSFAQAADTVKVYNWSDYIAPDTAKNFEKASGIGVTYDVYDSNETL
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEECCCCCC
DGKLMTGKSGYDVVFPSNHFMARQIEGGALKKLDKSQLPNWKNLNPVLLKALQTNDPGNE
CCEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
HGFPYLWGSTGIGYNVAKVKAVLGDNAPVDSWDLIFKPEYMEKLQKCGVAILDNGPELLP
CCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCEEEECCCHHHHH
AALNYLGLPHHSKNPEDYKKAEALLMKVRPYVSYFHSSKYTSDLANGDICVAVGFSGDIL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCEE
QAENRAKEAKNGIDIGYEIPKEGAAIWFDMVAMPADAPDEKAGYAFMNYLLQPDVMAGIS
HHHHHHHHHHCCCCCCEECCCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
NYVHYANGNEQADSLIDPAIKNDTKVYPSPEMMGKLFALEAMPLNIDRIRTRVWNKIRTG
HHEEECCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
S
C
>Mature Secondary Structure
MIRKTLALAPLMLAVSFAQAADTVKVYNWSDYIAPDTAKNFEKASGIGVTYDVYDSNETL
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEECCCCCC
DGKLMTGKSGYDVVFPSNHFMARQIEGGALKKLDKSQLPNWKNLNPVLLKALQTNDPGNE
CCEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
HGFPYLWGSTGIGYNVAKVKAVLGDNAPVDSWDLIFKPEYMEKLQKCGVAILDNGPELLP
CCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCEEEECCCHHHHH
AALNYLGLPHHSKNPEDYKKAEALLMKVRPYVSYFHSSKYTSDLANGDICVAVGFSGDIL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCEE
QAENRAKEAKNGIDIGYEIPKEGAAIWFDMVAMPADAPDEKAGYAFMNYLLQPDVMAGIS
HHHHHHHHHHCCCCCCEECCCCCCEEEEEEHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
NYVHYANGNEQADSLIDPAIKNDTKVYPSPEMMGKLFALEAMPLNIDRIRTRVWNKIRTG
HHEEECCCCHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]