Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 77458844

GI number: 77458844

Start: 2997942

End: 2998955

Strand: Direct

Name: ilvA [H]

Synonym: Pfl01_2619

Alternate gene names: 77458844

Gene position: 2997942-2998955 (Clockwise)

Preceding gene: 77458843

Following gene: 77458846

Centisome position: 46.56

GC content: 62.72

Gene sequence:

>1014_bases
ATGCGCTACAGCCCCGCCCAGCAAGCCCTGCTTGAGCAGTACGTGAAAAAGATCCTCGCCGCTCCGGTGTATGAACTGGC
GGTGCGCACGCCGCTGCAACCGGCGCCGGCGCTGTCCGAAGCGCTGGACAACCGGATCCTGCTCAAGCGCGAAGACCTGC
AACCGACGTTCTCCTTCAAGATCCGTGGTGCCTACAACAAACTGGTGCAGCTGAGTGATGAGCAGAAGGCGCGCGGAGTA
ATCACGGCGTCGGCGGGCAATCATGCGCAAGGCGTAGCGCTGGCGGCGCGGGAACTGGGTATTGCCGCGACCATTGTGAT
GCCGGCAACCACGCCTGAGTTGAAAGTGCTGGGCGTCAGTAGTCGCGGAGCCGAGGCGCTATTGCATGGAGAAAGCTTCC
CCTTTGCCCTGGCTCACGCGCTGCAACTGGCTGAGCAGACCGGCCGAACGTTCGTCTCGCCATTCGACGATCCCGATGTG
ATCGCCGGCCAGGGCACGGTCGCGATGGAAATCCTGCGCGAGCACCAAGGCACACTGGATGCGATCTTCGTCCCGGTGGG
CGGTGGCGGGTTGATCGCCGGCATCGCGGCGTATGTGAAATACCTGCGGCCTGAAGTACGGATCATCGGCGTCGAGTCGG
AGCACTCGGCCTGCCTGAAGGCTGCCATGCTAGCGGATGAGCGAGTGGTGCTGCCCAACGTGGGCACATTCGCCGATGGC
GTGGCGGTGGCGCAGATCGGCGCTTACGGGTTCGAGGTTTGCCGCTTCTGCGTGGATGAGGTCATCACCGTCAGCAACGA
CGAACTCTGCGCGGCGATCAAGAATATCTACGACGATACCCGCTCGATCACCGAGCCTTCCGGTGCTTTGGCGGTGGCGG
GCATTAAAAAGTACGTGGCGCAAAGCGGCGCACGGAGTCAGACGCTGATCGCCATCGATTCTGGCGCCAATATCAATTTC
GACAGCTTGCGACACGTCGCCGAGCGAGCGGCGGCGTGCGGCGTTGTGGCTTGA

Upstream 100 bases:

>100_bases
ATTTGGCGGGCTAGCTCACGACTGCAGCACCCAACCCGCCCTAGAGGCGGGTTTTTATTTTCTGTCTCCGGGGTTCTGAA
TCAAACGTCAGGAGACGACC

Downstream 100 bases:

>100_bases
AGTTACTCGGGAATGGCCACGCCAATCTTGTCCGAGGCCGACCAGATGCGGAAACGCACTTCAACGTCCTGCGGTGCGTA
AACCACGATCGGCAGCTTGC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MRYSPAQQALLEQYVKKILAAPVYELAVRTPLQPAPALSEALDNRILLKREDLQPTFSFKIRGAYNKLVQLSDEQKARGV
ITASAGNHAQGVALAARELGIAATIVMPATTPELKVLGVSSRGAEALLHGESFPFALAHALQLAEQTGRTFVSPFDDPDV
IAGQGTVAMEILREHQGTLDAIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESEHSACLKAAMLADERVVLPNVGTFADG
VAVAQIGAYGFEVCRFCVDEVITVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKKYVAQSGARSQTLIAIDSGANINF
DSLRHVAERAAACGVVA

Sequences:

>Translated_337_residues
MRYSPAQQALLEQYVKKILAAPVYELAVRTPLQPAPALSEALDNRILLKREDLQPTFSFKIRGAYNKLVQLSDEQKARGV
ITASAGNHAQGVALAARELGIAATIVMPATTPELKVLGVSSRGAEALLHGESFPFALAHALQLAEQTGRTFVSPFDDPDV
IAGQGTVAMEILREHQGTLDAIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESEHSACLKAAMLADERVVLPNVGTFADG
VAVAQIGAYGFEVCRFCVDEVITVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKKYVAQSGARSQTLIAIDSGANINF
DSLRHVAERAAACGVVA
>Mature_337_residues
MRYSPAQQALLEQYVKKILAAPVYELAVRTPLQPAPALSEALDNRILLKREDLQPTFSFKIRGAYNKLVQLSDEQKARGV
ITASAGNHAQGVALAARELGIAATIVMPATTPELKVLGVSSRGAEALLHGESFPFALAHALQLAEQTGRTFVSPFDDPDV
IAGQGTVAMEILREHQGTLDAIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESEHSACLKAAMLADERVVLPNVGTFADG
VAVAQIGAYGFEVCRFCVDEVITVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKKYVAQSGARSQTLIAIDSGANINF
DSLRHVAERAAACGVVA

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=301, Percent_Identity=34.21926910299, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI19923959, Length=308, Percent_Identity=30.1948051948052, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI33469958, Length=318, Percent_Identity=29.559748427673, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1790207, Length=317, Percent_Identity=54.8895899053628, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI1789505, Length=312, Percent_Identity=39.1025641025641, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789235, Length=229, Percent_Identity=25.3275109170306, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=303, Percent_Identity=36.6336633663366, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537387, Length=309, Percent_Identity=36.2459546925566, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=267, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320930, Length=323, Percent_Identity=52.3219814241486, Blast_Score=305, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6322631, Length=272, Percent_Identity=38.6029411764706, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319788, Length=248, Percent_Identity=27.0161290322581, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355833, Length=257, Percent_Identity=38.5214007782101, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24645328, Length=179, Percent_Identity=35.7541899441341, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 35623; Mature: 35623

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSPAQQALLEQYVKKILAAPVYELAVRTPLQPAPALSEALDNRILLKREDLQPTFSFK
CCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCEEEE
IRGAYNKLVQLSDEQKARGVITASAGNHAQGVALAARELGIAATIVMPATTPELKVLGVS
EECHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCEEEEEEC
SRGAEALLHGESFPFALAHALQLAEQTGRTFVSPFDDPDVIAGQGTVAMEILREHQGTLD
CCCCCEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCHHHHHHHHHCCCCEE
AIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESEHSACLKAAMLADERVVLPNVGTFADG
EEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCHHCC
VAVAQIGAYGFEVCRFCVDEVITVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKKYVA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QSGARSQTLIAIDSGANINFDSLRHVAERAAACGVVA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRYSPAQQALLEQYVKKILAAPVYELAVRTPLQPAPALSEALDNRILLKREDLQPTFSFK
CCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCEEEE
IRGAYNKLVQLSDEQKARGVITASAGNHAQGVALAARELGIAATIVMPATTPELKVLGVS
EECHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCEEEEEEC
SRGAEALLHGESFPFALAHALQLAEQTGRTFVSPFDDPDVIAGQGTVAMEILREHQGTLD
CCCCCEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCHHHHHHHHHCCCCEE
AIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESEHSACLKAAMLADERVVLPNVGTFADG
EEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCHHCC
VAVAQIGAYGFEVCRFCVDEVITVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKKYVA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QSGARSQTLIAIDSGANINFDSLRHVAERAAACGVVA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA