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The map label for this gene is sir2 [H]

Identifier: 77458738

GI number: 77458738

Start: 2869755

End: 2871428

Strand: Direct

Name: sir2 [H]

Synonym: Pfl01_2513

Alternate gene names: 77458738

Gene position: 2869755-2871428 (Clockwise)

Preceding gene: 77458735

Following gene: 77458739

Centisome position: 44.57

GC content: 62.54

Gene sequence:

>1674_bases
ATGTATGAGTACGATGATTACGACCGGGCGCTGGTGTTCGAGCGCGTTGCGCAATTTCGCGATCAGGTCGAGCGCTTCAT
GGCCGGGGAGTTGAGCGAAGAGGAATTCCTGCCGTTGCGCCTGCAAAACGGCCTGTACATGCAAAAGCACGCGTACATGC
TGCGGGTGGCCATTCCTTACGGCACGCTGAGTGCCGAGCAGATGCGCACACTGGCAAGCATCGCCCGGGACTACGACCGT
GGTTACGGCCACTTCACCACCCGGCAGAACATGCAGTTCAACTGGATCGAGCTGCACGAGGTGCCGGACATTCTCGAGCG
TCTGGCGCAGGTCAACATGCATGCGATCCAGACCTCGGGCAATTGCGTGCGCAACATCACCACCGAAGCCTTCGCCGGGG
TCGCGGCGGACGAACTGATCGACCCGCGTCCGCTGGCGGAAATCCTGCGGCAATGGTCGACGATCAACCCGGAATTCCTG
TTCCTGCCGCGCAAGTTCAAGATCGCCATCTGCTCGGCGAAGCAGGATCGCGCGGCGATCATGATGCACGACATCGGTCT
CTACCTTTACCCCGGTCGTGACGGCCAGATGCTGCTGCGGGTGATCGTCGGCGGCGGTCTGGGGCGCACGCCGATTCTCG
GTCTGCAAATTCGCGATGGCCTGCCGTGGCAGCATTTGCTGTCCTATGTCGAGGCGGTGTTGCGGGTCTACAATCGCCAC
GGCCGGCGCGACAACAAGTACAAGGCGCGGATCAAGATTCTGGTCAAGGCGCTGGGCATCGAGGCGTTCGCCAAAGAGGT
CGAAGAGGAATGGCAACACCTCAAGGACGGACCGGCGCAATTGACCGAGGATGAATACCAGCGCGTCGCCAGTGCCTTCG
TGCCGCCGAGCTATCGCACATTGGCGGGCACCGATCTGGATTTCGGCACGCATCTGGCGGAGAACCCGGCGTTCGCCCGC
TGGGTTGCGCGCAACGTTCAGCCGCACAAGGTGCCGGGCTACACCAGCGTGGTGCTGTCGACCAAACCGGGCCTGGCCGC
GCCGCCGGGGGACGTCACCGATGCGCAGATGCAGGCCGTGGCCGACTGGTCCGAGCGTTTCGGTTTCGGCGAAATCCGCA
TCGCCCACGAGCAGAACATCGTCTTGCCCGACGTGCCCAAGTCCGAGCTGTTTGCGCTGTGGCAGCAGGCGAAAGAGCAG
GGGCTGGGCGCCGCCAATGTCGGCCTGCTGACCGACATCATTGCCTGCCCCGGCGGTGATTTCTGTGCGCTGGCCAATGC
CAAATCGATCCCGATTGCCCAGGCGATTCAGGCGCGTTTCGACAACCTCGATTACCTGCATGATCTGGGCGACATCAGCC
TGAATATCTCCGGCTGCATGAACGCCTGCGGCCATCACCACATCGGCAACATCGGGATTCTCGGCGTCGATAAAAACGGC
AGCGAGTGGTACCAGATCACCCTCGGCGGCGCGCAGGGCAAAAACAGCGCACTGGGCAAAGTCATCGGCCCGTCGTTCAG
CGCCGCCGAAGTGCCGCAGGTGATCGAGCGGATCATCGGCACCTTCGTCCGTTATCGCGAGGCCGAAGAACAGTTCGTCG
AGACCGTGGCGCGCATCGGACTGGAGCCGTTCAAGGAGCGGGTCTACCCGAAAGCGCTGGAGGTGTCGGCATGA

Upstream 100 bases:

>100_bases
AACGGGCGAACCCGAGATAGCGCCAGATGCGAACTGGCGCTATCTCGGGTTCGCCCGTTCCCACAGGAATTCATGTAATT
CCTGATGAGGTAGTAGGCCG

Downstream 100 bases:

>100_bases
ACAATCTGTTGCGCATTGAGGCGAGTAGGGCGCGGATCGTGCTCGATGATCCGTGGACGCTGATCCGCTCGCCCGAAACG
GCTGACAGCACTGAAATGTT

Product: nitrite and sulfite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MYEYDDYDRALVFERVAQFRDQVERFMAGELSEEEFLPLRLQNGLYMQKHAYMLRVAIPYGTLSAEQMRTLASIARDYDR
GYGHFTTRQNMQFNWIELHEVPDILERLAQVNMHAIQTSGNCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFL
FLPRKFKIAICSAKQDRAAIMMHDIGLYLYPGRDGQMLLRVIVGGGLGRTPILGLQIRDGLPWQHLLSYVEAVLRVYNRH
GRRDNKYKARIKILVKALGIEAFAKEVEEEWQHLKDGPAQLTEDEYQRVASAFVPPSYRTLAGTDLDFGTHLAENPAFAR
WVARNVQPHKVPGYTSVVLSTKPGLAAPPGDVTDAQMQAVADWSERFGFGEIRIAHEQNIVLPDVPKSELFALWQQAKEQ
GLGAANVGLLTDIIACPGGDFCALANAKSIPIAQAIQARFDNLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
SEWYQITLGGAQGKNSALGKVIGPSFSAAEVPQVIERIIGTFVRYREAEEQFVETVARIGLEPFKERVYPKALEVSA

Sequences:

>Translated_557_residues
MYEYDDYDRALVFERVAQFRDQVERFMAGELSEEEFLPLRLQNGLYMQKHAYMLRVAIPYGTLSAEQMRTLASIARDYDR
GYGHFTTRQNMQFNWIELHEVPDILERLAQVNMHAIQTSGNCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFL
FLPRKFKIAICSAKQDRAAIMMHDIGLYLYPGRDGQMLLRVIVGGGLGRTPILGLQIRDGLPWQHLLSYVEAVLRVYNRH
GRRDNKYKARIKILVKALGIEAFAKEVEEEWQHLKDGPAQLTEDEYQRVASAFVPPSYRTLAGTDLDFGTHLAENPAFAR
WVARNVQPHKVPGYTSVVLSTKPGLAAPPGDVTDAQMQAVADWSERFGFGEIRIAHEQNIVLPDVPKSELFALWQQAKEQ
GLGAANVGLLTDIIACPGGDFCALANAKSIPIAQAIQARFDNLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
SEWYQITLGGAQGKNSALGKVIGPSFSAAEVPQVIERIIGTFVRYREAEEQFVETVARIGLEPFKERVYPKALEVSA
>Mature_557_residues
MYEYDDYDRALVFERVAQFRDQVERFMAGELSEEEFLPLRLQNGLYMQKHAYMLRVAIPYGTLSAEQMRTLASIARDYDR
GYGHFTTRQNMQFNWIELHEVPDILERLAQVNMHAIQTSGNCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFL
FLPRKFKIAICSAKQDRAAIMMHDIGLYLYPGRDGQMLLRVIVGGGLGRTPILGLQIRDGLPWQHLLSYVEAVLRVYNRH
GRRDNKYKARIKILVKALGIEAFAKEVEEEWQHLKDGPAQLTEDEYQRVASAFVPPSYRTLAGTDLDFGTHLAENPAFAR
WVARNVQPHKVPGYTSVVLSTKPGLAAPPGDVTDAQMQAVADWSERFGFGEIRIAHEQNIVLPDVPKSELFALWQQAKEQ
GLGAANVGLLTDIIACPGGDFCALANAKSIPIAQAIQARFDNLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
SEWYQITLGGAQGKNSALGKVIGPSFSAAEVPQVIERIIGTFVRYREAEEQFVETVARIGLEPFKERVYPKALEVSA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=565, Percent_Identity=23.1858407079646, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62278; Mature: 62278

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEYDDYDRALVFERVAQFRDQVERFMAGELSEEEFLPLRLQNGLYMQKHAYMLRVAIPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCEEEEEEEECC
GTLSAEQMRTLASIARDYDRGYGHFTTRQNMQFNWIELHEVPDILERLAQVNMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHEEECCC
NCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFLFLPRKFKIAICSAKQDRAAI
CHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCEE
MMHDIGLYLYPGRDGQMLLRVIVGGGLGRTPILGLQIRDGLPWQHLLSYVEAVLRVYNRH
EEEECCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRRDNKYKARIKILVKALGIEAFAKEVEEEWQHLKDGPAQLTEDEYQRVASAFVPPSYRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
LAGTDLDFGTHLAENPAFARWVARNVQPHKVPGYTSVVLSTKPGLAAPPGDVTDAQMQAV
CCCCCCCCCCHHCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
ADWSERFGFGEIRIAHEQNIVLPDVPKSELFALWQQAKEQGLGAANVGLLTDIIACPGGD
HHHHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
FCALANAKSIPIAQAIQARFDNLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
EEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCEEEHHHHHHHCCCCCCCCEEEEEECCCC
SEWYQITLGGAQGKNSALGKVIGPSFSAAEVPQVIERIIGTFVRYREAEEQFVETVARIG
CCEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEPFKERVYPKALEVSA
CHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MYEYDDYDRALVFERVAQFRDQVERFMAGELSEEEFLPLRLQNGLYMQKHAYMLRVAIPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCEEEEEEEECC
GTLSAEQMRTLASIARDYDRGYGHFTTRQNMQFNWIELHEVPDILERLAQVNMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHEEECCC
NCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFLFLPRKFKIAICSAKQDRAAI
CHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCEE
MMHDIGLYLYPGRDGQMLLRVIVGGGLGRTPILGLQIRDGLPWQHLLSYVEAVLRVYNRH
EEEECCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRRDNKYKARIKILVKALGIEAFAKEVEEEWQHLKDGPAQLTEDEYQRVASAFVPPSYRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
LAGTDLDFGTHLAENPAFARWVARNVQPHKVPGYTSVVLSTKPGLAAPPGDVTDAQMQAV
CCCCCCCCCCHHCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
ADWSERFGFGEIRIAHEQNIVLPDVPKSELFALWQQAKEQGLGAANVGLLTDIIACPGGD
HHHHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
FCALANAKSIPIAQAIQARFDNLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
EEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCEEEHHHHHHHCCCCCCCCEEEEEECCCC
SEWYQITLGGAQGKNSALGKVIGPSFSAAEVPQVIERIIGTFVRYREAEEQFVETVARIG
CCEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEPFKERVYPKALEVSA
CHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA