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The map label for this gene is kipI [H]

Identifier: 77457633

GI number: 77457633

Start: 1577391

End: 1578095

Strand: Direct

Name: kipI [H]

Synonym: Pfl01_1406

Alternate gene names: 77457633

Gene position: 1577391-1578095 (Clockwise)

Preceding gene: 77457632

Following gene: 77457634

Centisome position: 24.5

GC content: 62.55

Gene sequence:

>705_bases
ATGAATCCGCGGGTGGAAGTGGTGGCGCTGGATTGCCTGATGCTGCGCTTGTTCGATGAAATCGCCGAAGCCAACATGCC
GTGGATGCTCGCCGCCAGCGAGCGGTTGCGCGCGTTGTTCGGCGCACATCTGATCGATCTGGTGCCGTCGTACACCACGT
TGATGGTGCATTACGATCTGACCGCGTTGAGCCCGAATCAGGCGCGGGAGTTGATCGCCGAAGCGCTGATCGACCTGTCG
CCGAATGCGCGTATCGGCGGCCAGTGCCATGTGCTGCCGGTGTGGTACGACCTGAGCGTCGGACCGGAATTGAGTTTGCT
GTCTCAACGCAGCGGCTTGGCAGTAGAGGAGGTGATCCGCCGCCACAGTGCCCGTGAATATCAGGTGTTCGCGCTCGGAT
TCGCACCGGGGTTTGCCTTCATGGGCCTGGTGGAAGAAGTCCTCGCGGCGCCGCGTCTGAATACCCCACGCAAGAAAGTC
GCCGCCGGCAGTGTCGGCATCGCCGAGCGTCAGACAGCGGCGTATCCGGTGGTGTCCCCCGGTGGCTGGAACCTGATCGG
TCGCACGCCGGCAAAGTTGTTCGACCGTGAACGCGATGGCTACAGCCTCATGCAACCCGGCGATACCGTACGTTTCGAAG
CGGTAAGCCATGCCGAATTCATCCGCCTCGGCGGTGACGACACGCCTCTGGAGGCCCTCGCATGA

Upstream 100 bases:

>100_bases
TGCACCTGCAAGCCAACACTTTGTGCGTGCACGGTGACAACGCCAGCTCAGTGGCGGCCGTGCAGCGCATTCGTCAGGCC
TTGAACGAGCAGAGCGCGCC

Downstream 100 bases:

>100_bases
GCCGACTGACGATTGAAGCGAGTACGCCGCTGTGCCTGTTGCAGGATGCCGGCCGGTTTGGCGTACGGCATCTGGGCGTG
ACCCAGGGTGGCGCGGCGGA

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MNPRVEVVALDCLMLRLFDEIAEANMPWMLAASERLRALFGAHLIDLVPSYTTLMVHYDLTALSPNQARELIAEALIDLS
PNARIGGQCHVLPVWYDLSVGPELSLLSQRSGLAVEEVIRRHSAREYQVFALGFAPGFAFMGLVEEVLAAPRLNTPRKKV
AAGSVGIAERQTAAYPVVSPGGWNLIGRTPAKLFDRERDGYSLMQPGDTVRFEAVSHAEFIRLGGDDTPLEALA

Sequences:

>Translated_234_residues
MNPRVEVVALDCLMLRLFDEIAEANMPWMLAASERLRALFGAHLIDLVPSYTTLMVHYDLTALSPNQARELIAEALIDLS
PNARIGGQCHVLPVWYDLSVGPELSLLSQRSGLAVEEVIRRHSAREYQVFALGFAPGFAFMGLVEEVLAAPRLNTPRKKV
AAGSVGIAERQTAAYPVVSPGGWNLIGRTPAKLFDRERDGYSLMQPGDTVRFEAVSHAEFIRLGGDDTPLEALA
>Mature_234_residues
MNPRVEVVALDCLMLRLFDEIAEANMPWMLAASERLRALFGAHLIDLVPSYTTLMVHYDLTALSPNQARELIAEALIDLS
PNARIGGQCHVLPVWYDLSVGPELSLLSQRSGLAVEEVIRRHSAREYQVFALGFAPGFAFMGLVEEVLAAPRLNTPRKKV
AAGSVGIAERQTAAYPVVSPGGWNLIGRTPAKLFDRERDGYSLMQPGDTVRFEAVSHAEFIRLGGDDTPLEALA

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=121, Percent_Identity=47.9338842975207, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 25640; Mature: 25640

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRVEVVALDCLMLRLFDEIAEANMPWMLAASERLRALFGAHLIDLVPSYTTLMVHYDL
CCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
TALSPNQARELIAEALIDLSPNARIGGQCHVLPVWYDLSVGPELSLLSQRSGLAVEEVIR
EECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCCHHHHHH
RHSAREYQVFALGFAPGFAFMGLVEEVLAAPRLNTPRKKVAAGSVGIAERQTAAYPVVSP
HCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCEECC
GGWNLIGRTPAKLFDRERDGYSLMQPGDTVRFEAVSHAEFIRLGGDDTPLEALA
CCCEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHCC
>Mature Secondary Structure
MNPRVEVVALDCLMLRLFDEIAEANMPWMLAASERLRALFGAHLIDLVPSYTTLMVHYDL
CCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
TALSPNQARELIAEALIDLSPNARIGGQCHVLPVWYDLSVGPELSLLSQRSGLAVEEVIR
EECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCCHHHHHH
RHSAREYQVFALGFAPGFAFMGLVEEVLAAPRLNTPRKKVAAGSVGIAERQTAAYPVVSP
HCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCEECC
GGWNLIGRTPAKLFDRERDGYSLMQPGDTVRFEAVSHAEFIRLGGDDTPLEALA
CCCEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]