Click here to switch to the map view.

The map label for this gene is insF1 [C]

Identifier: 77457574

GI number: 77457574

Start: 1522042

End: 1522839

Strand: Direct

Name: insF1 [C]

Synonym: Pfl01_1347

Alternate gene names: 77457574

Gene position: 1522042-1522839 (Clockwise)

Preceding gene: 77457573

Following gene: 77457576

Centisome position: 23.64

GC content: 54.14

Gene sequence:

>798_bases
ATGGGGATTTCCCGTCAGGCTTATTACAAGCGCAACCGCGCGTGTGATGCCCGGGCCCGTCATGCCCAGGAAGTGATGGG
ATTCGTGAGGGAGAAACGACTTAGGCAACCGCGCCTTGGCACCCGAAAACTTCACAATCTGATGCACACTGAGCCAGAGG
TGTCCGTAAAGGTCGGTCGAGACCGGTTATTCGATATCTTGCGAGATCGGCGCGAGCTGGTTCCCCGCAGACGGGCCTAT
CACAAAACGACAGATAGCCATCATCGCTTTCGCCGGCATCCAAACCTGCTCAAAGATGGACCGATTCAGGTAGTTGCCAA
GGCGCCAGAGCAAGTATGGGTTGCGGACATCACCTACCTACCGACGCAAACGGGTGTGGCTTATCTGAGTTTGATCACCG
ATGCGTACTCCCGAAAAATCGTGGGGCACCATGTCCATGAAAGCTTGCACACCGAATCGGTGATCCAGGCGTTCAACAAA
GCCCTCAAGCAGCGGACAACGAAGCAATGTTTGGTGCATCACTCGGATAGAGGCGTCCAATACTGCTCCGAGCTTTATCA
GCGGCTGCATGCCAAATACGGCATCACCTGCTCAATGACCGATGGCTACGACTGCTACCAAAACGCGTTGGCTGAGCGTG
TGAACGGGATTTTGAAAAACGAGTTATTGCTGCATCGCCCCAAAAATTTTGCAGAGGCCATCCGGATGGTGGACGAGTCG
GTGCAGATCTATAACAACGAGCGGCCTCATCTGTCGCTGAAATACAAAACACCCGATGCGGTGCATCGGGCGTTTTGA

Upstream 100 bases:

>100_bases
TAAAGAACGACTTCGGTGTTTCTGTCGTAAAAAAGCGATCCGGCAAGTCCTCTCCCAAGGGCAGATCCAAGACCTGAGCA
TTAGTCGGGCTTGCCAGTTC

Downstream 100 bases:

>100_bases
GGCTGAAACAGGTGTAAACCTATTTCAGGACTAGACATGGATTTCAGTAACTGGAGAGATCCGCCAACGGATGCCGCCCC
TCCCAGACTTTGTGAAAGTG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MGISRQAYYKRNRACDARARHAQEVMGFVREKRLRQPRLGTRKLHNLMHTEPEVSVKVGRDRLFDILRDRRELVPRRRAY
HKTTDSHHRFRRHPNLLKDGPIQVVAKAPEQVWVADITYLPTQTGVAYLSLITDAYSRKIVGHHVHESLHTESVIQAFNK
ALKQRTTKQCLVHHSDRGVQYCSELYQRLHAKYGITCSMTDGYDCYQNALAERVNGILKNELLLHRPKNFAEAIRMVDES
VQIYNNERPHLSLKYKTPDAVHRAF

Sequences:

>Translated_265_residues
MGISRQAYYKRNRACDARARHAQEVMGFVREKRLRQPRLGTRKLHNLMHTEPEVSVKVGRDRLFDILRDRRELVPRRRAY
HKTTDSHHRFRRHPNLLKDGPIQVVAKAPEQVWVADITYLPTQTGVAYLSLITDAYSRKIVGHHVHESLHTESVIQAFNK
ALKQRTTKQCLVHHSDRGVQYCSELYQRLHAKYGITCSMTDGYDCYQNALAERVNGILKNELLLHRPKNFAEAIRMVDES
VQIYNNERPHLSLKYKTPDAVHRAF
>Mature_264_residues
GISRQAYYKRNRACDARARHAQEVMGFVREKRLRQPRLGTRKLHNLMHTEPEVSVKVGRDRLFDILRDRRELVPRRRAYH
KTTDSHHRFRRHPNLLKDGPIQVVAKAPEQVWVADITYLPTQTGVAYLSLITDAYSRKIVGHHVHESLHTESVIQAFNKA
LKQRTTKQCLVHHSDRGVQYCSELYQRLHAKYGITCSMTDGYDCYQNALAERVNGILKNELLLHRPKNFAEAIRMVDESV
QIYNNERPHLSLKYKTPDAVHRAF

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=136, Percent_Identity=37.5, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788405, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787263, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786753, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786570, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786490, Length=118, Percent_Identity=34.7457627118644, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789981, Length=166, Percent_Identity=30.7228915662651, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30983; Mature: 30852

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISRQAYYKRNRACDARARHAQEVMGFVREKRLRQPRLGTRKLHNLMHTEPEVSVKVGR
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEHHH
DRLFDILRDRRELVPRRRAYHKTTDSHHRFRRHPNLLKDGPIQVVAKAPEQVWVADITYL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHEEECCCCCEEHHHHEEC
PTQTGVAYLSLITDAYSRKIVGHHVHESLHTESVIQAFNKALKQRTTKQCLVHHSDRGVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YCSELYQRLHAKYGITCSMTDGYDCYQNALAERVNGILKNELLLHRPKNFAEAIRMVDES
HHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VQIYNNERPHLSLKYKTPDAVHRAF
HHHHCCCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure 
GISRQAYYKRNRACDARARHAQEVMGFVREKRLRQPRLGTRKLHNLMHTEPEVSVKVGR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEHHH
DRLFDILRDRRELVPRRRAYHKTTDSHHRFRRHPNLLKDGPIQVVAKAPEQVWVADITYL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHEEECCCCCEEHHHHEEC
PTQTGVAYLSLITDAYSRKIVGHHVHESLHTESVIQAFNKALKQRTTKQCLVHHSDRGVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YCSELYQRLHAKYGITCSMTDGYDCYQNALAERVNGILKNELLLHRPKNFAEAIRMVDES
HHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VQIYNNERPHLSLKYKTPDAVHRAF
HHHHCCCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]