Click here to switch to the map view.

The map label for this gene is pcaK [H]

Identifier: 77457493

GI number: 77457493

Start: 1439224

End: 1440570

Strand: Direct

Name: pcaK [H]

Synonym: Pfl01_1266

Alternate gene names: 77457493

Gene position: 1439224-1440570 (Clockwise)

Preceding gene: 77457492

Following gene: 77457494

Centisome position: 22.35

GC content: 63.85

Gene sequence:

>1347_bases
ATGAACCAGCCTCAGTCCGCTGTAGGTCACTGCCTCGACGTGCAGTCCTTCATCAATGCCCAACCGATCTCGCGCTATCA
GTGGCGCGTGGTGATCCTGTGTTTCCTGATTGTGTTCCTCGATGGCCTCGACACCGCGGCCATGGGTTTTATCGCGCCGG
CCCTGTCCCAGGACTGGGGCATCGACCGCGCCAGCCTCGGCCCGGTGATGAGTGCCGCGCTGATCGGCATGGTCTTCGGC
GCGCTGGGCTCCGGTCCGCTGGCTGACCGCTTCGGGCGAAAAGTCGTACTGGTCGGCGCGGTATTTCTGTTCGGCGCCTT
CAGCCTGGCTTCGGCCTACGCCACCAACGTCGATCAACTGCTGGTACTGCGCTTCCTCACCGGTCTGGGACTGGGCGCGG
GGATGCCGAACGCCACCACGCTGTTGTCGGAATACACCCCGGAGCGCAAGAAGTCATTGCTGGTGACCAGCATGTTCTGC
GGCTTCAACCTGGGCATGGCCGGCGGTGGATTCATCTCGGCCAAGCTGATCCCGGCATTCGGCTGGCACAGCCTGTTGCT
GATCGGCGGGATCCTGCCGCTGATCCTCACCGTGGTATTGCTGCTCTGGCTGCCGGAATCGGCGCGCTATCTGGTGGTGC
GCAATCGCGGCACTGACAAGGTGCGCAAGACTCTCGCACCCATTGATCCTGCCATCGTCGCTCAGGCCGCGAGTTTCAGC
GTGCCGGAACAGAAAACCGTCAAAGCGCGCAACGTGTTCGCGGTGATTTTCTCCGGTACCTACAGCGTCGGCACGTTGCT
GCTGTGGCTGACCTACTTCATGGGCCTGGTGATTGTTTACCTGCTGACCAGCTGGCTGCCGACCCTGATGCGCGACAGCG
GCGCGAGCATGGAGCAGGCGGCGTTTATCGGCGCGCTGTTCCAGTTCGGCGGGGTGTTGAGTGCGGTCGGCGTGGGCTGG
GCGATGGACCGCTTCAATCCGCACAAGGTCATCGGCATTTTCTACCTGCTGGCCGGGGTTTTTGCCTACGCGGTGGGGCA
GAGTCTGGGCAACATCACGCTGCTGGCGACGTTGGTGCTGGTGGCGGGCATGTGTGTCAACGGTGCGCAATCGGCGATGC
CGTCGCTGGCAGCGCGGTTTTATCCGACGCAGGGCCGGGCGACCGGTGTCTCGTGGATGCTCGGGATCGGCCGCTTCGGC
GCGATTCTCGGGGCGTGGATGGGCGCAACCCTGTTGGGCCTGGGCTGGAATTTCGAACAGGTGCTGACAGCGTTGGTGAT
CCCGGCCGGGCTGGCCACGGCAGCGGTGGTGATCAAGGGCCTGGTCAGTCACGCGGACGCAACCTGA

Upstream 100 bases:

>100_bases
GTATTAGCGCAACGCCTGATTTCTTCCTTATATAGTTGTGACCGTGCCGCTCTCTGGCCGGCCGCCGTTCGACTGCGTTT
TCTTGCGTGGAATAAAAATA

Downstream 100 bases:

>100_bases
TATCCACGTCCATCACTCCCGCACGCGGTGCGGGAGTGATCATTAGGCAGATAACAATCTGTTCGATAAACGAACACTCA
GTCGATTATCGGATTGTTTG

Product: benzoate transport

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNQPQSAVGHCLDVQSFINAQPISRYQWRVVILCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVFG
ALGSGPLADRFGRKVVLVGAVFLFGAFSLASAYATNVDQLLVLRFLTGLGLGAGMPNATTLLSEYTPERKKSLLVTSMFC
GFNLGMAGGGFISAKLIPAFGWHSLLLIGGILPLILTVVLLLWLPESARYLVVRNRGTDKVRKTLAPIDPAIVAQAASFS
VPEQKTVKARNVFAVIFSGTYSVGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQAAFIGALFQFGGVLSAVGVGW
AMDRFNPHKVIGIFYLLAGVFAYAVGQSLGNITLLATLVLVAGMCVNGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFG
AILGAWMGATLLGLGWNFEQVLTALVIPAGLATAAVVIKGLVSHADAT

Sequences:

>Translated_448_residues
MNQPQSAVGHCLDVQSFINAQPISRYQWRVVILCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVFG
ALGSGPLADRFGRKVVLVGAVFLFGAFSLASAYATNVDQLLVLRFLTGLGLGAGMPNATTLLSEYTPERKKSLLVTSMFC
GFNLGMAGGGFISAKLIPAFGWHSLLLIGGILPLILTVVLLLWLPESARYLVVRNRGTDKVRKTLAPIDPAIVAQAASFS
VPEQKTVKARNVFAVIFSGTYSVGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQAAFIGALFQFGGVLSAVGVGW
AMDRFNPHKVIGIFYLLAGVFAYAVGQSLGNITLLATLVLVAGMCVNGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFG
AILGAWMGATLLGLGWNFEQVLTALVIPAGLATAAVVIKGLVSHADAT
>Mature_448_residues
MNQPQSAVGHCLDVQSFINAQPISRYQWRVVILCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVFG
ALGSGPLADRFGRKVVLVGAVFLFGAFSLASAYATNVDQLLVLRFLTGLGLGAGMPNATTLLSEYTPERKKSLLVTSMFC
GFNLGMAGGGFISAKLIPAFGWHSLLLIGGILPLILTVVLLLWLPESARYLVVRNRGTDKVRKTLAPIDPAIVAQAASFS
VPEQKTVKARNVFAVIFSGTYSVGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQAAFIGALFQFGGVLSAVGVGW
AMDRFNPHKVIGIFYLLAGVFAYAVGQSLGNITLLATLVLVAGMCVNGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFG
AILGAWMGATLLGLGWNFEQVLTALVIPAGLATAAVVIKGLVSHADAT

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=426, Percent_Identity=25.8215962441315, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI7662270, Length=334, Percent_Identity=25.1497005988024, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI262399383, Length=281, Percent_Identity=25.9786476868327, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI213021148, Length=293, Percent_Identity=25.2559726962457, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI73695465, Length=203, Percent_Identity=30.0492610837438, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI166064021, Length=173, Percent_Identity=29.4797687861272, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI87081723, Length=388, Percent_Identity=37.1134020618557, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082231, Length=419, Percent_Identity=24.8210023866348, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788074, Length=362, Percent_Identity=25.9668508287293, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082159, Length=401, Percent_Identity=24.6882793017456, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082404, Length=377, Percent_Identity=23.0769230769231, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789312, Length=221, Percent_Identity=28.5067873303167, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789152, Length=332, Percent_Identity=22.5903614457831, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786229, Length=215, Percent_Identity=22.3255813953488, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789207, Length=267, Percent_Identity=24.7191011235955, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=429, Percent_Identity=23.0769230769231, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922874, Length=416, Percent_Identity=25.4807692307692, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24640198, Length=459, Percent_Identity=23.9651416122004, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640196, Length=459, Percent_Identity=23.9651416122004, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640200, Length=459, Percent_Identity=23.9651416122004, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161077756, Length=447, Percent_Identity=22.5950782997763, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47352; Mature: 47352

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]