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The map label for this gene is yafC [C]

Identifier: 77457466

GI number: 77457466

Start: 1410960

End: 1411931

Strand: Direct

Name: yafC [C]

Synonym: Pfl01_1239

Alternate gene names: 77457466

Gene position: 1410960-1411931 (Clockwise)

Preceding gene: 77457462

Following gene: 77457476

Centisome position: 21.91

GC content: 60.91

Gene sequence:

>972_bases
GTGGTTTTTTCTGCGGATAAGCGCACTAATGGGCAACCTTTGGTTTTCAAATGGAAACAATCCATGAGCGAGATGGATGA
TCTGGCGGCGTTCGCGGTGTTGATCGAAGCCGGCAGTTTTACCTTGGCGGCGCAGCAATTGGGGTGCAGCAAGGGGCAGT
TGTCCAAGCGCATCAGTCAGTTGGAGGCGCGGTTTTCCGTGGTGTTGCTGCAACGCACCACGCGTCGGCTGAGCCTGACG
GCGGCCGGTGCGGCGTTGTTGCCACAGGCTCAGGCGCTTGTCGTCCAGGTGGAGCGAGCGCGTCAGGCATTGGCGCGATT
GAAGGACGACATGGCCGGCCCGGTGCGTATGACGGTTCCGGTGTCGCTGGGGGAAACCTTCTTCGACGGTCTGCTGCTGG
ATTTCTCGCAGAAATACCCCGAGGTACAGATCGAACTGGAGCTGAATAACAGCTACCGCGACCTGTCTCGCGACAGTTTC
GATCTGGCGATCCGCACCGAGGTGGCCAATGACGAGCGACTGGTGGCCAAGCCGCTGCTGGCCTGGCAGGAAATGACCTG
CGCCAGTCCTGAATATCTGGAGCGCTTTGGCGAACCGCTGACGCCGCAGGCGCTGGCCGAGCACCGTTGCCTGCTCAACA
GTCATTACAGCGGTCGCGAGGAATGGCTCTATCACCAGCAGCACGAACTGCTGCGGGTGCGGGTGTCGGGGCCGTTCGCC
AGCAATCACTATAACCTGTTGAAGAAAGCCGCATTGGCCGGCGCCGGCATCGCTCGCCTGCCGTCCTACCTGTTGCAGGC
GGAATTGGCTGACGGCCGATTGCGCTGGCTCCTTCGCGATTTTCAGACCCGACGCATGCCGATGTATCTGGTGCACCCGT
ATCAGGGCGGTCTACCGAAACGCACCCAGGTGCTGGCAGATTACCTGATCGGCTGGTTCAAGCTCAGCGGCGAGGCGCTG
GACCGGTTGTAG

Upstream 100 bases:

>100_bases
CAGCTCTTTGTCCACGGCCGAACCGATGGTGCCGCCCGCGCCGATCAACAGAATTTTCATCGTGATTGCCTCAAGTGATT
GAACGAGGTTTCAGTCTAGA

Downstream 100 bases:

>100_bases
TGCCTGGCGATCAGGTGATCGATCGACAGTTTGCCTGGCCCGGTCGCCATCAGGTACAGCAACACCGCCATCCAGGTGCC
GTGGGTCGGGTACGCATCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVFSADKRTNGQPLVFKWKQSMSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQLEARFSVVLLQRTTRRLSLT
AAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVPVSLGETFFDGLLLDFSQKYPEVQIELELNNSYRDLSRDSF
DLAIRTEVANDERLVAKPLLAWQEMTCASPEYLERFGEPLTPQALAEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFA
SNHYNLLKKAALAGAGIARLPSYLLQAELADGRLRWLLRDFQTRRMPMYLVHPYQGGLPKRTQVLADYLIGWFKLSGEAL
DRL

Sequences:

>Translated_323_residues
MVFSADKRTNGQPLVFKWKQSMSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQLEARFSVVLLQRTTRRLSLT
AAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVPVSLGETFFDGLLLDFSQKYPEVQIELELNNSYRDLSRDSF
DLAIRTEVANDERLVAKPLLAWQEMTCASPEYLERFGEPLTPQALAEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFA
SNHYNLLKKAALAGAGIARLPSYLLQAELADGRLRWLLRDFQTRRMPMYLVHPYQGGLPKRTQVLADYLIGWFKLSGEAL
DRL
>Mature_323_residues
MVFSADKRTNGQPLVFKWKQSMSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQLEARFSVVLLQRTTRRLSLT
AAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVPVSLGETFFDGLLLDFSQKYPEVQIELELNNSYRDLSRDSF
DLAIRTEVANDERLVAKPLLAWQEMTCASPEYLERFGEPLTPQALAEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFA
SNHYNLLKKAALAGAGIARLPSYLLQAELADGRLRWLLRDFQTRRMPMYLVHPYQGGLPKRTQVLADYLIGWFKLSGEAL
DRL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=30.4195804195804, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=28.956228956229, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=26.7796610169492, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=26.0416666666667, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789440, Length=256, Percent_Identity=28.125, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=28.0769230769231, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=28.6231884057971, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=26.5993265993266, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082132, Length=259, Percent_Identity=29.3436293436293, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=259, Percent_Identity=25.0965250965251, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=29.4776119402985, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786448, Length=300, Percent_Identity=25.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=30.718954248366, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790399, Length=261, Percent_Identity=23.7547892720307, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36494; Mature: 36494

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSADKRTNGQPLVFKWKQSMSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQ
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHH
LEARFSVVLLQRTTRRLSLTAAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVP
HHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VSLGETFFDGLLLDFSQKYPEVQIELELNNSYRDLSRDSFDLAIRTEVANDERLVAKPLL
HHHCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCCCHHHHHHHH
AWQEMTCASPEYLERFGEPLTPQALAEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFA
HHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCC
SNHYNLLKKAALAGAGIARLPSYLLQAELADGRLRWLLRDFQTRRMPMYLVHPYQGGLPK
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCH
RTQVLADYLIGWFKLSGEALDRL
HHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MVFSADKRTNGQPLVFKWKQSMSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQ
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHH
LEARFSVVLLQRTTRRLSLTAAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVP
HHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
VSLGETFFDGLLLDFSQKYPEVQIELELNNSYRDLSRDSFDLAIRTEVANDERLVAKPLL
HHHCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCCCHHHHHHHH
AWQEMTCASPEYLERFGEPLTPQALAEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFA
HHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCC
SNHYNLLKKAALAGAGIARLPSYLLQAELADGRLRWLLRDFQTRRMPMYLVHPYQGGLPK
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCH
RTQVLADYLIGWFKLSGEALDRL
HHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]