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The map label for this gene is pcm [H]

Identifier: 77457357

GI number: 77457357

Start: 1306541

End: 1307218

Strand: Direct

Name: pcm [H]

Synonym: Pfl01_1130

Alternate gene names: 77457357

Gene position: 1306541-1307218 (Clockwise)

Preceding gene: 77457356

Following gene: 77457358

Centisome position: 20.29

GC content: 62.39

Gene sequence:

>678_bases
ATGGCTCGTGAACAAGACGACAGGCTGCGCAGCGGCATCGGGATGACGTCCCAGCGAACCCGTGAGCGCCTGATCCAGCG
TCTGTACGAAGAGGGCGTTTCCAACGCCAGCGTGCTGGAAGTGATCCGCCGCACCCCGCGACACCTGTTCGTCGATGAAG
CGCTGGCGCATCGTGCCTATGAAGACACCGCGCTGCCGATCGGCAACAACCAGACCATTTCCCAGCCTTATATGGTGGCG
CGCATGAGCGAGCTGCTGCTGGAAGCCGGGCCGCTGGATAAGGTGCTGGAGATCGGCACCGGATCCGGTTACCAGACGGC
AGTGTTGTCGCAGTTGGTCGAGCGGGTGTTCTCGGTAGAGCGCATCAAGGTGCTGCAGGATCGGGCCAAGGAACGCCTGA
TCGAGCTCAACCTGCGCAACGTGGTATTTCGCTGGGGCGATGGTTGGGAAGGGTGGCCGGCGCTAGCGCCGTACAACGGC
ATCATCGTCACGGCGGTCGCCACCGATGTGCCTCAGGCTTTGCTTGATCAACTGGCACCGGGTGGGCGGATGGTGATCCC
GGTCGGTTCGGGCGAAGTGCAGCAATTGATGCTGATCGTGCGCGAAGAAAACGGTTTCTCCCGACACGTTCTGGGGGCGG
TGCGTTTCGTGCCATTGCTCAACGGTCCGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGGTGATGCAGGGTTACGTATCCATCACTCCGTTGCAGCTCGATCGCACCTTCAATGATGCCTTCAGAAGTCTCGACGGC
TGGCTGGAGGGGCTCAACTG

Downstream 100 bases:

>100_bases
GCATTTGTTCGAGGCCGCTGAATTCCTGTCGAACGCGCCTGTCTTACAGCGGCGGCGAGTGTTTAAACAGAAAGGATCGT
CTACAAGCAAACGTGTGCGC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MAREQDDRLRSGIGMTSQRTRERLIQRLYEEGVSNASVLEVIRRTPRHLFVDEALAHRAYEDTALPIGNNQTISQPYMVA
RMSELLLEAGPLDKVLEIGTGSGYQTAVLSQLVERVFSVERIKVLQDRAKERLIELNLRNVVFRWGDGWEGWPALAPYNG
IIVTAVATDVPQALLDQLAPGGRMVIPVGSGEVQQLMLIVREENGFSRHVLGAVRFVPLLNGPLA

Sequences:

>Translated_225_residues
MAREQDDRLRSGIGMTSQRTRERLIQRLYEEGVSNASVLEVIRRTPRHLFVDEALAHRAYEDTALPIGNNQTISQPYMVA
RMSELLLEAGPLDKVLEIGTGSGYQTAVLSQLVERVFSVERIKVLQDRAKERLIELNLRNVVFRWGDGWEGWPALAPYNG
IIVTAVATDVPQALLDQLAPGGRMVIPVGSGEVQQLMLIVREENGFSRHVLGAVRFVPLLNGPLA
>Mature_224_residues
AREQDDRLRSGIGMTSQRTRERLIQRLYEEGVSNASVLEVIRRTPRHLFVDEALAHRAYEDTALPIGNNQTISQPYMVAR
MSELLLEAGPLDKVLEIGTGSGYQTAVLSQLVERVFSVERIKVLQDRAKERLIELNLRNVVFRWGDGWEGWPALAPYNGI
IVTAVATDVPQALLDQLAPGGRMVIPVGSGEVQQLMLIVREENGFSRHVLGAVRFVPLLNGPLA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=216, Percent_Identity=35.1851851851852, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=52.60663507109, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71983477, Length=172, Percent_Identity=37.7906976744186, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=169, Percent_Identity=34.9112426035503, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=32.8767123287671, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25064; Mature: 24933

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREQDDRLRSGIGMTSQRTRERLIQRLYEEGVSNASVLEVIRRTPRHLFVDEALAHRAY
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
EDTALPIGNNQTISQPYMVARMSELLLEAGPLDKVLEIGTGSGYQTAVLSQLVERVFSVE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RIKVLQDRAKERLIELNLRNVVFRWGDGWEGWPALAPYNGIIVTAVATDVPQALLDQLAP
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GGRMVIPVGSGEVQQLMLIVREENGFSRHVLGAVRFVPLLNGPLA
CCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AREQDDRLRSGIGMTSQRTRERLIQRLYEEGVSNASVLEVIRRTPRHLFVDEALAHRAY
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
EDTALPIGNNQTISQPYMVARMSELLLEAGPLDKVLEIGTGSGYQTAVLSQLVERVFSVE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RIKVLQDRAKERLIELNLRNVVFRWGDGWEGWPALAPYNGIIVTAVATDVPQALLDQLAP
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GGRMVIPVGSGEVQQLMLIVREENGFSRHVLGAVRFVPLLNGPLA
CCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]