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The map label for this gene is lepA [H]

Identifier: 77457219

GI number: 77457219

Start: 1153445

End: 1155241

Strand: Direct

Name: lepA [H]

Synonym: Pfl01_0992

Alternate gene names: 77457219

Gene position: 1153445-1155241 (Clockwise)

Preceding gene: 77457218

Following gene: 77457220

Centisome position: 17.92

GC content: 58.54

Gene sequence:

>1797_bases
GTGAGTGATTTGAGTCATATCCGCAATTTCTCCATCATCGCCCACATTGACCATGGCAAGTCGACGCTGGCTGACCGTTT
CATCCAGATGTGCGGCGGCCTGGCCGAGCGTGAAATGGAAGCCCAGGTACTGGATTCCATGGATCTGGAGCGCGAACGCG
GGATCACCATCAAGGCCCACAGCGTCACCCTCTATTACAAGGCCAAAGACGGCGTCACCTACCAGCTGAACTTCATTGAC
ACCCCGGGTCACGTCGACTTCACCTACGAAGTCAGCCGTTCCCTGGCGGCCTGTGAAGGTGCGTTGCTGGTGGTCGATGC
GGGGCAGGGCGTTGAAGCCCAGTCCGTGGCCAACTGCTACACCGCCATCGAGCAGGGCCTTGAGGTCATGCCGGTGCTGA
ACAAGATCGACCTGCCACAGGCCGAGCCCGATCGCGTCAAGGACGAGATCGAGAAGATCATCGGCATCGACGCCACCGAC
GCCGTCACTTGCAGCGCCAAGACCGGCCTGGGTGTCGATGAAGTACTCGAGCGTCTGGTCGCTACCATTCCTGCGCCGAT
CGGCAACATCGAAGATCCGCTGCAAGCGTTGATCATCGACTCCTGGTTCGACAACTACCTGGGCGTAGTCTCCCTGGTGC
GCGTGCGTCACGGCCGCGTGAAGAAGGGCGACAAGATCCTGGTGAAGTCCACCGGCAAAGTGCATCTGGTCGACAGCGTC
GGCGTGTTCAACCCGAAACACACCGCCACCGCTGACCTGAAGGCCGGCGAAGTGGGCTTCATCATCGCCAGCATCAAGGA
CATTCACGGTGCGCCGGTCGGTGACACCCTGACCCTGAGCTCCACTCCGGACGTTCCGGTGCTGCCAGGTTTCAAACGCA
TCCAGCCGCAGGTTTACGCCGGTCTGTTCCCGGTCAGTTCCGACGATTTCGAGGACTTCCGCGAAGCGTTGCAGAAACTG
ACCCTGAACGACTCGTCGCTGCAATACACCCCGGAAAGCTCCGACGCACTGGGCTTCGGCTTCCGTTGCGGCTTCCTCGG
CATGCTGCACATGGAGATCATCCAGGAGCGCCTGGAGCGCGAATACGACCTGGACCTGATCACCACGGCGCCAACCGTAA
TCTTCGAACTGGTGCTGAAAACCGGTGAAACGATTTACGTGGACAACCCGTCGAAGCTGCCGGACGTCTCGTCGATCGAA
GACATGCGCGAGCCGATCGTGCGCGCCAACATCCTGGTACCGCAGGAACACCTGGGCAACGTCATCACTCTGTGCATCGA
GAAACGCGGTGTGCAGGTCGACATGCTGTTCCTCGGCAATCAGGTGCAAGTGACCTACGACTTGCCGATGAACGAAGTGG
TCCTGGACTTCTTCGACCGTCTGAAATCCACCAGCCGCGGCTATGCTTCGCTGGACTACCATTTCGATCGTTACCAATCG
GCTAATCTGGTGAAACTGGACGTGCTGATCAACGGCGACAAGGTCGACGCTCTGGCGCTGATCGTGCACCGTGACAATTC
GCACTTCAAAGGTCGCCAGTTGACCGAGAAGATGAAAGAACTGATTCCTCGTCAGATGTTCGACGTGGCCATCCAGGCTG
CCATTGGCGGTCAGATCGTCGCCCGGACAACCGTCAAGGCACTCAGAAAGAACGTATTGGCCAAGTGCTACGGCGGCGAC
GTCAGCCGTAAGAAAAAACTGCTTGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTCGGCAACGTGGAAATTCC
ACAAGAAGCCTTCCTCGCCGTGCTCAGGTTGGAATAG

Upstream 100 bases:

>100_bases
TCCGGGTGACAGGCGAACGTGACGCTTGCGCCGTGTATCAGGTACAATTCCCGGCTATTTTTCGGCGGGCCAAGCCTGCA
GCCTTTTTGAGTGTTGATCC

Downstream 100 bases:

>100_bases
TCAGGTCCTATGTCACTAAATTTCCCGCTGTTGCTGGTCATTGCCGTGTTCGTCTGCGGTCTGTTGGCGTTGCTCGATCT
GTTGATCCTGGCACCGCGTC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGVTYQLNFID
TPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKIDLPQAEPDRVKDEIEKIIGIDATD
AVTCSAKTGLGVDEVLERLVATIPAPIGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKVHLVDSV
GVFNPKHTATADLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVPVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKL
TLNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVIFELVLKTGETIYVDNPSKLPDVSSIE
DMREPIVRANILVPQEHLGNVITLCIEKRGVQVDMLFLGNQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQS
ANLVKLDVLINGDKVDALALIVHRDNSHFKGRQLTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGD
VSRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE

Sequences:

>Translated_598_residues
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGVTYQLNFID
TPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKIDLPQAEPDRVKDEIEKIIGIDATD
AVTCSAKTGLGVDEVLERLVATIPAPIGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKVHLVDSV
GVFNPKHTATADLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVPVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKL
TLNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVIFELVLKTGETIYVDNPSKLPDVSSIE
DMREPIVRANILVPQEHLGNVITLCIEKRGVQVDMLFLGNQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQS
ANLVKLDVLINGDKVDALALIVHRDNSHFKGRQLTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGD
VSRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
>Mature_597_residues
SDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGVTYQLNFIDT
PGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKIDLPQAEPDRVKDEIEKIIGIDATDA
VTCSAKTGLGVDEVLERLVATIPAPIGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKVHLVDSVG
VFNPKHTATADLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVPVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKLT
LNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVIFELVLKTGETIYVDNPSKLPDVSSIED
MREPIVRANILVPQEHLGNVITLCIEKRGVQVDMLFLGNQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQSA
NLVKLDVLINGDKVDALALIVHRDNSHFKGRQLTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGDV
SRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.0066225165563, Blast_Score=623, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=37.3493975903614, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=39.5973154362416, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=37.9746835443038, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI217272892, Length=173, Percent_Identity=30.0578034682081, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI217272894, Length=173, Percent_Identity=30.0578034682081, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI53729339, Length=244, Percent_Identity=28.2786885245902, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI53729337, Length=244, Percent_Identity=28.2786885245902, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI4503471, Length=226, Percent_Identity=29.2035398230088, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=73.7373737373737, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=28.7128712871287, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=35.8490566037736, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=31.4410480349345, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=28.8343558282209, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=42.1311475409836, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=161, Percent_Identity=33.5403726708075, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=35.5072463768116, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=35.5072463768116, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=33.5403726708075, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=29.8755186721992, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=299, Percent_Identity=28.0936454849498, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.3488372093023, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=39.5973154362416, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=39.5973154362416, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=36.875, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=39.3162393162393, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=37.5838926174497, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=122, Percent_Identity=35.2459016393443, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=122, Percent_Identity=35.2459016393443, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.9081803005008, Blast_Score=534, Evalue=1e-152,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=42.3357664233577, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=35.9477124183007, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=35.9477124183007, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=35.9477124183007, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221458488, Length=154, Percent_Identity=39.6103896103896, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357743, Length=163, Percent_Identity=31.9018404907975, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28572034, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66161; Mature: 66030

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAH
CCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYKAKDGVTYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
EEEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TAIEQGLEVMPVLNKIDLPQAEPDRVKDEIEKIIGIDATDAVTCSAKTGLGVDEVLERLV
HHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHH
ATIPAPIGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKVHLVDSV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECC
GVFNPKHTATADLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVPVLPGFKRIQPQVYA
CCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHH
GLFPVSSDDFEDFREALQKLTLNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLER
EECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EYDLDLITTAPTVIFELVLKTGETIYVDNPSKLPDVSSIEDMREPIVRANILVPQEHLGN
HCCCEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHEEECCHHHHCC
VITLCIEKRGVQVDMLFLGNQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQS
EEEEEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCHHHHCCC
ANLVKLDVLINGDKVDALALIVHRDNSHFKGRQLTEKMKELIPRQMFDVAIQAAIGGQIV
CCEEEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARTTVKALRKNVLAKCYGGDVSRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHCCC
>Mature Secondary Structure 
SDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAH
CCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYKAKDGVTYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
EEEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TAIEQGLEVMPVLNKIDLPQAEPDRVKDEIEKIIGIDATDAVTCSAKTGLGVDEVLERLV
HHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHH
ATIPAPIGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKVHLVDSV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECC
GVFNPKHTATADLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVPVLPGFKRIQPQVYA
CCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHH
GLFPVSSDDFEDFREALQKLTLNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLER
EECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EYDLDLITTAPTVIFELVLKTGETIYVDNPSKLPDVSSIEDMREPIVRANILVPQEHLGN
HCCCEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHEEECCHHHHCC
VITLCIEKRGVQVDMLFLGNQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQS
EEEEEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCHHHHCCC
ANLVKLDVLINGDKVDALALIVHRDNSHFKGRQLTEKMKELIPRQMFDVAIQAAIGGQIV
CCEEEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARTTVKALRKNVLAKCYGGDVSRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA