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The map label for this gene is argT [H]

Identifier: 77457194

GI number: 77457194

Start: 1126985

End: 1127761

Strand: Direct

Name: argT [H]

Synonym: Pfl01_0967

Alternate gene names: 77457194

Gene position: 1126985-1127761 (Clockwise)

Preceding gene: 77457193

Following gene: 77457200

Centisome position: 17.5

GC content: 58.43

Gene sequence:

>777_bases
ATGAAGAAATTCCCCCTCATTACCGGTCTGGCGCTGAGCCTGTTGGCGTGCAGCAGCGCGTTCGCCGCCGAGAAAACCCT
GCGTATCGGTATCGAAGCGGCGTATCCGCCCTTCGCCTCGAAAACCGACAAGGGTGAAATCGTCGGTTTTGACTACGACA
TCGGCAATGCCCTGTGCGCGCAAATGCAGGTCAAGTGCGTGTGGGTCGAGGGTGAGTTCGACGGTCTGATTCCTTCCCTG
AAAGTGAAGAAAATCGACATGGCGCTGTCGTCCATGACCATCAATGAAGACCGCAAGAAGTCGGTGGACTTCACCCACAA
GTACTATTTCACCTCGTCGCGGCTGGTGATGAAGGAGGGCGCGAGCGTCGATGACCAGTACGCCAGCCTCAAGGGCAAAA
CCGTCGGCGTGCAGCGTGCGACCACCACCGACCGTTACGCCACCGAGGTGTTCGAGCCCAAGGGCATCAACGTCAAGCGT
TACGGCAACAACGAAGAAATCTACATGGACCTGGCGGCCGGGCGTCTCGATGCGATCTTCGCCGACACCATCCCGTTGAC
CGACTTCCTGTCGATGCCGCGCGGCAAGGGTTACGCGTTTGTCGGGCCTGAGCTGAAGGATCCGAAGTACGTGGGCGAGG
GCGCGGGGATTGCGGTGCGCAAGGGCAACACAGAACTGGTCAGCCAGTTGAACACTGCCATCGACGGTATTCGCGCCAGT
GGCGAGTACCAGAAGATTTCCGAGAAGTACTTCAAGTCCGATATCTACGGCGACTGA

Upstream 100 bases:

>100_bases
AAAAGTCCAGTATGGATTTACAAGTACAAAAAAGGCCTGTTCCCGTTTCAGCTTTTTCTTCTGTCTTGCCAAACCAACAA
CAACCCGCGAGAGAACGAAC

Downstream 100 bases:

>100_bases
ATCTGAAAACCGTGCAGCCTTCGCAGGCTGCACGGTTTCAGCCCTTCAATTCCTTCAAATGCTTGTACACCGTGGCCCGC
CCCATGTTCAGTACATTGGC

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKFPLITGLALSLLACSSAFAAEKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALCAQMQVKCVWVEGEFDGLIPSL
KVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKEGASVDDQYASLKGKTVGVQRATTTDRYATEVFEPKGINVKR
YGNNEEIYMDLAAGRLDAIFADTIPLTDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSQLNTAIDGIRAS
GEYQKISEKYFKSDIYGD

Sequences:

>Translated_258_residues
MKKFPLITGLALSLLACSSAFAAEKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALCAQMQVKCVWVEGEFDGLIPSL
KVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKEGASVDDQYASLKGKTVGVQRATTTDRYATEVFEPKGINVKR
YGNNEEIYMDLAAGRLDAIFADTIPLTDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSQLNTAIDGIRAS
GEYQKISEKYFKSDIYGD
>Mature_258_residues
MKKFPLITGLALSLLACSSAFAAEKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALCAQMQVKCVWVEGEFDGLIPSL
KVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKEGASVDDQYASLKGKTVGVQRATTTDRYATEVFEPKGINVKR
YGNNEEIYMDLAAGRLDAIFADTIPLTDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSQLNTAIDGIRAS
GEYQKISEKYFKSDIYGD

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=45, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=44.4444444444444, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=39.5918367346939, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=40.4, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=31.3807531380753, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=32.1428571428571, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786876, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28349; Mature: 28349

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFPLITGLALSLLACSSAFAAEKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALCA
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHEE
QMQVKCVWVEGEFDGLIPSLKVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKEG
EEEEEEEEEECCCCCCCCCCEEEHHHHHHHHEECCCCHHCCCCEEEEEEEECCEEEEECC
ASVDDQYASLKGKTVGVQRATTTDRYATEVFEPKGINVKRYGNNEEIYMDLAAGRLDAIF
CCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCEEEEECCCCEEEEEECCCCEEEEE
ADTIPLTDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSQLNTAIDGIRAS
ECCCCHHHHHCCCCCCCEEEECCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCC
GEYQKISEKYFKSDIYGD
CCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKFPLITGLALSLLACSSAFAAEKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALCA
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHEE
QMQVKCVWVEGEFDGLIPSLKVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKEG
EEEEEEEEEECCCCCCCCCCEEEHHHHHHHHEECCCCHHCCCCEEEEEEEECCEEEEECC
ASVDDQYASLKGKTVGVQRATTTDRYATEVFEPKGINVKRYGNNEEIYMDLAAGRLDAIF
CCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCEEEEECCCCEEEEEECCCCEEEEE
ADTIPLTDFLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSQLNTAIDGIRAS
ECCCCHHHHHCCCCCCCEEEECCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCC
GEYQKISEKYFKSDIYGD
CCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]